A possible correlation of Ezrin and Fascin-1 in adenoid cystic carcinoma of salivary gland: an immunohistochemical study
Nadia
Radi
Lecturer of Oral and Dental Pathology, Faculty of Dental Medicine for Girls. Al Azhar University.
author
Gihan
balbola
Lecturer of Oral and Dental Pathology, Faculty of Dental Medicine for Girls. Al Azhar University.
author
text
article
2018
eng
Adenoid cystic carcinoma (ACC) of salivary gland is one of the most common epithelial salivary gland carcinomas and is characterized by extensive local tissue infiltration and poor long-term survival. All forms of ACC are characterized by prolonged but persistent growth, extensive perineural invasion and hematogenous metastases. Distant sites of metastasis are primarily lung, although bone and liver are also common. The incremental motility of malignant cells is a critical step in their migration, invasion and metastasis, which is regulated by reorganization of actin cytoskeleton and regulation of focal adhesion. Fascin-1 and ezrin are essential components of these cellular structures. The aim of this study is to evaluate the expression of these cytoskeleton-associated proteins in ACC as a prognostic indicator of invasiveness. Five cases of normal salivary gland tissue and 43 cases of adenoid cystic carcinoma (10 cases cribriform form, 15 cases tubular form and 18 cases solid form). Immunohistochmical staining for ezrin and fascin-1 antibodies were done for all specimens. Statistical analysis was performed using a commercially available software program (SPSS 19; SPSS, Chicago, IL, USA). The level of significance was set at P < 0.05. For both markers, statistically, the greatest mean area percent was recorded in solid pattern ACC, whereas the lowest values were recorded in normal tissue. These results indicating a synergistic effect between ezrin and fascin-1 during ACC invasion.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2105
2112
https://edj.journals.ekb.eg/article_76764_9cf97a70030ba63c5d15c2d558f24825.pdf
dx.doi.org/10.21608/edj.2018.76764
Assessment of the Gingival Zenith Positions and Levels of the Maxillary Anterior Teeth among Saudi population: Comparison between males and females
Badr
Bamusa
Lecturer of Periodontology, Riyadh Elm University
author
Alqarni
Amal
Dental Intern, Riyadh Elm University
author
Manal
Al-Alsheikh
Dental Intern, Riyadh Elm University.
author
Albalwe
Alanood
Dental Intern, Riyadh Elm University.
author
Yara
Altasan
Dental Intern, Riyadh Elm University
author
Hind
Alkhaibari
Dental Intern, Riyadh Elm University
author
Hafez
Diab
Professor of periodontology, Riyadh Elm University
author
text
article
2018
eng
Introduction: Understanding of the dentogingival interface allows the clinicians to achieve a more satisfactory esthetic outcome during interdisciplinary diagnosis and treatment planning of beautiful smile. Aim: To evaluate the gingival variations in the maxillary anterior teeth by quantifying the following esthetic parameters; marginal gingival zenith positions (MGZP), gingival zenith level (GZL) and to assess the difference of these parameters among males and females.Materials & Methods: A total of 20 maxillary casts (10 males and 10 females) were analyzed. Digital caliper and protractor was used to measure; the marginal Gingival Zenith Position (MGZP) from the vertical bisected midline (VBM) of 120 sites in the maxillary anterior teeth, the Gingival Zenith Level (GZL) of 40 sites related to the lateral incisors, the data were analyzed using Mann Whitney u test.Results: According to the mean, all the maxillary anterior teeth displayed distal (MGZP) from (VBM), with mean average of 1 mm in the central incisors, 0.4 mm in the lateral incisors, and 0.2 mm in the canines among the study samples. Comparisons between males and females presented no statistically significant differences. The Gingival zenith level (GZL) showed statistically significant differences between right and left teeth in males and females and revealed positive values in all 40 sites (100%) when appeared coronal to the gingival aesthetic line. The mean of the males and females (GZL) in mm were 1.05 and 0.95 respectively with no statistically significant differences between both gender. Conclusions: The marginal gingival zenith position (MGZP) is located distally in all anterior teeth. The gingival zenith line (GZL) was found to be coronal to the gingival aesthetic line in all teeth with significant differences between right and left sides within each group (males, females). In all recorded parameters there were no differences between males and females. These clinical parameters applied to the gingival contours may serve as reference points during esthetic anterior oral rehabilitation.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2113
2119
https://edj.journals.ekb.eg/article_76769_0542c1eca0c05d27a17409f82a1412a8.pdf
dx.doi.org/10.21608/edj.2018.76769
The evaluation of the chemopreventive effect of β-carotene (A-Viton®) on oral premalignant lesions through the estimation of the expression of p53 levels
Shaymaa
Hassan
Lecturer in Department of Oral Medicine and Periodontology, Cairo University, Faculty of Dentistry
author
text
article
2018
eng
Background: Oral premalignant lesions (OPL) include a wide range of lesions including leukoplakia, dysplastic leukoplakia, erythroplakia, oral submucous fibrosis, dysplastic lichenoid lesions, and oral lichen planus. Proper diagnosis and management of oral premalignant lesions (OPL) are very important; as 17% of these lesions have been reported to be transformed into malignant lesions within first 7 years of their diagnosis. Vitamin A has always been known for its chemopreventive effect in several diseases through the modulation of cell proliferation and, it is also known for being a potent antioxidant and anti-inflammatory agent. Any alterations in p53 protein results in its accumulation in the cell nuclei; may play a critical role in carcinogenesis, including oral premalignant lesions and oral malignant lesions, i.e., p53 protein is one of the determinants in the progression of oral dysplasia to invasive malignancy.Methods: Fifteen patients having oral premalignant lesions of mild and moderate dysplasia; diagnosed both clinically and histologically, were given a chemopreventive therapy in the form of βeta- carotene (Vit. A) for four months. The levels of p53 protein immunostaining were measured pre- and post-treatment.Results: The mean value of optical staining density of P53 in pre-treatment specimen was (59.78 ± 10.22) compared to (39.74 ± 3.36) in post-treatment cases; this value was higher in pre-treatment cases than post-treatment cases. The p-value was 0.0003.Thus when statistically compared the difference is considered statistically significant.Conclusion: The expression of P53 protein in (OPL) is found to be inversely related to the clinical as well as histopathological response to beta-carotene supplementation.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2121
2129
https://edj.journals.ekb.eg/article_76770_42749df4ce04e200fde467e0ab8ade5a.pdf
dx.doi.org/10.21608/edj.2018.76770
The potential role of CD31 and Bcl-2 in the etiopathogenesis of oral pyogenic granuloma
Naglaa
Kamal
Lecturer, Oral Pathology Department, Faculty of Oral and Dental Medicine, Ahram Canadian University, 6th of
October City, Giza Egypt
author
text
article
2018
eng
Pyogenic granuloma (PG) is a vascular reactive lesion of the oral cavity which is caused by local trauma or irritation and has a distinct biological behavior. Up till now, the pathogenesis of PG is not adequately understood. Aim: Assessment of the expression of CD31 and Bcl-2 in PG to clarify the etiopathogenesis of this lesion and to determine whether vascular proliferation in this lesion has a relation to its apoptotic status. Materials and Methods: 4μm sections were prepared from paraffin-embedded blocks of PG (20 samples) and normal gingiva as a control (10 samples). Staining of each specimen with hematoxylin and eosin (H&E), CD31 and Bcl-2 was done. Results: all lesions of PG showed positive CD31 and Bcl-2 immunostaining in the endothelial cells of blood vessels and in the stromal ovoid cells. Control group showed positive CD31expression in the endothelial cells of blood vessels while Bcl-2 expression revealed positive expression mainly in the basal cells of the epithelium. A significant higher expression of CD31 and Bcl-2 was detected in PG than normal gingiva. A strong positive correlation was found between CD31 and Bcl-2 area percent of immune-expression. Conclusions: The data of the present study suggest a role of Bcl-2 overexpression in the potentiation of angiogenic response and support the role of CD31 and Bcl-2 in the etiopathogenesis of PG.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2131
2140
https://edj.journals.ekb.eg/article_76771_eb6aebd5bd54fb38adf9e7f00340a37b.pdf
dx.doi.org/10.21608/edj.2018.76771
Modulatory Effect of Mushroom Extract on 7, 12-Dimethyle Anthracene (DMBA)-Induced Lip Cancer in Albino Rats
Marwa
Abd El Hameed
Lecturer of Oral Biology, Faculty of Dentistry Ain Shams University.
author
Dina
AbdelKhalik
Lecturer of Oral Biology, Faculty of Dentistry Ain Shams University.
author
Rehab
Fathi
Lecturer of Oral Pathology, Faculty of Dentistry Ain Shams University
author
text
article
2018
eng
Background: Oral cancer constitutes approximately 25% of all cancer types. 7, 12-dimethylbenz (a) anthracene (DMBA) has been widely used as an active chemical inducer for mammary carcinogenesis. COX-2 enzyme is frequently found in certain types of cancer, including colon, lung, breast, pancreas, head, and neck cancers and is usually associated with poor prognosis and short survival. Mushrooms contain polysaccharides that are thought to inhibit tumor growth and viral infection by stimulating immune cells. Objective: The present work aimed to investigate the effect of mushroom extract on DMBA-induced lip carcinogenesis in albino rats and correlate the results with COX-2 tissue expression.Materials and methods: Thirty-six adult female albino ratswere divided into three groups as follows (twelve rats each); Group 1: (–ve control group in which each rat was given 1 ml sesame oil via gastric gavage as a single weekly dose for 6 weeks). Group 2: DMBA (+ve control group in which each rat was given DMBA emulsion via gastric gavage as a single weekly dose for 6 weeks). Group 3: DMBA+M (Experimental group in which each rat was given DMBA emulsion for 3 weeks and was co-administered with mushroom extract (dissolved in distilled water) for the following 3 weeks via gastric gavage. Lip specimens were processed for hematoxylin and eosin staining and immunohistochemically prepared for COX-2 expression. The area fraction expressed by COX-2 was calculated histomorphometrically. The data was expressed statistically as mean ± standard deviation (S.D.).Results: Both mucous membrane and skin side of Group 2 specimens showed signs of basement membrane rupture as well as hyperchromatic, pleomorphic and dysplastic epithelial cells. Connective tissue showed invasionwith dysplastic epithelial cells, dilated and congested blood vessels with many inflammatory cells. However, the mucous membrane side Group 3 exhibited almost intact basement membrane and only few hyperchromatic epithelial cells. The connective tissue displayed less inflammatory cell infiltration with almost absent invasion while, skin side of Group 3 presented some epithelial areas with intracellular edema but apparently minimal invasion of epithelial cells in the dermal connective tissue.The immunoexpression pattern of COX-2 in both mucous membrane and skin side of Group 2 was clear and strongly positive in epithelial and connective tissue cells. However, Group 3 showed down regulated expression of COX-2.Statistical results presented a significantly reduced expression of COX-2 in Group 1 followed by Group 3, while the highest expression was shown in Group 2.Conclusion: Mushroom extract is a possible natural source that may limit the carcinogenic effect of a known carcinogenic agent by suppressingits inflammatory reaction and invasive power.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2141
2154
https://edj.journals.ekb.eg/article_76773_918caac5e77ff054ae8b32c2cf16ed59.pdf
dx.doi.org/10.21608/edj.2018.76773
Evaluation of leptin levels in the serum and gingival cevicular fluid of chronic periodontitis patients
Ali
Salem
Dental intern Practitioner, Faculty of Dentistry, Umm Al-Qura University, KSA
author
Naif
Al-Harthi
Dental intern Practitioner, Faculty of Dentistry, Umm Al-Qura University, KSA
author
Ahmed
Dardir
Assistant Professor of Periodontology, Department of Oral Medicine and Periodontology, Ahram Canadian
University, Egypt and Umm Al-Qura University, KSA.
author
Abdel-Rahman
Youssef
Assistant Professor of Microbiology and Immunology, Faculty of Medicine, Umm Al-Qura University, Saudi
Arabia and Suez Canal University Egypt.
author
text
article
2018
eng
Leptin is pro-inflammatory mediator associated with the pathogenesis of periodontitis. The objective of the current study was to assess the levels of leptin in serum and gingival crevicular fluid (GCF) in chronic periodontitis (CP) patients. This cross-sectional study was conducted in Dental Teaching Hospital, Umm Al-Qura University, Makkah, Saudi Arabia. Forty-five individuals participated in this study: 25 with chronic periodontitis patients and 20 periodontally healthy controls. The patients were selected based on the criteria of American Academy of Periodontology using probing depths, bleeding on probing, clinical attachment loss and radiographs. GCF and serum samples were collected to estimate the leptin concentrations using enzyme-linked immunosorbent assay kits. Serum leptin levels were markedly higher in chronic periodontitis patients than in the healthy group (P < 0.00001). Although GCF leptin levels of chronic periodontitis patients were higher than the healthy group but this difference was not statistically significant (P Value = 0.5198). In conclusion, these results indicate positive association between serum leptin concentrations and chronic periodontitis. However, there was no significant correlation between GCF leptin level and periodontitis. Further studies are required to confirm these finding.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2155
2159
https://edj.journals.ekb.eg/article_76779_2ad51690cb1db02d724fb47256ce2bf3.pdf
dx.doi.org/10.21608/edj.2018.76779
Biological effect of Egyptian Propolis on BHK cell line and on dental pulp tissue (An In vitro And In vivo study)
Marwa
Moussa
Lecturer of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University
author
Marwa
Temirek
Lecturer of Operative Dentistry, Faculty of Dentistry, Fayoum University
author
text
article
2018
eng
Background: Propolis is a natural material with different biological activities such as anti-inflammatory, antibacterial and antifungal action. The aim of the present work was to evaluate biocompatibility of Egyptian Propolis by using two methods of assessment: the invitro test using Baby hamster kidney cell (BHK) cell line and the invivio study on dental pulp of dogs. Materials & Methods: For the invitro study: ethanol extract of propolis (EEP) was prepared at a concentration of 1mg/ml. A suspension of the cultured BHK cells was placed in 96 multi-well dishes and divided into two groups : control group containing BHK cells without EEP and propolis group containing BHK cells with EEP at a concentration of 1mg/ml. Cell counting was performed at 24 hours, 72 hours and at the seventh day. Mean values were then calculated for each group. The results were then statistically analyzed using ANOVA test. Scanning Electron Microscopy (SEM) was done for both groups at the seventh day. For the invivo study, 2 young healthy adult dogs, of comparable age, 1-1.5 years old and complete set of permanent dentition were used in this study. The canine and first molar of each quadrant were used in this study and second upper molars of each dog was also prepared, forming a sum of 10 sound teeth. A standardized class V cavity was prepared on the facial surface of the selected teeth. All the cavities were restored with resin modified glass ionomer while in half of them Propolis was used as lining material under resin modified glass ionomer. At the end of experimental period after 6 months, dogs were sacrificed. Microsections with 5 μm width were prepared and stained with hematoxylin and eosin. Results: invitro study results showed a significant increase in number of BHK cells of propolis group at 72 hours and at the seventh day when compared to the control group. Scanning electron microscopic examination of BHK cells showed increase in density of BHK cells of propolis group as compared to cells of the control group. Both groups showed uniform spreading and attachment of cells to root surface. Examination of the decalcified stained sections of teeth of propolis group revealed no inflammation, normal tubular pattern of dentin and arrangement of odntoblasts in multiple layers . Presence of line of demarcation between primary and secondary dentine. Conclusion : Egyptian propolis stimulated growth and proliferation of BHK cell in vitro and showed no inflamatory effect on pulp tissue invivo.These results collectively proved the biocomptability and good biological properties of Egyptian propolis and support the application of Egyptian propolis in numerous fields of dentistry.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2161
2170
https://edj.journals.ekb.eg/article_76780_a4e3a46fad50c3af6060ee07a5886a83.pdf
dx.doi.org/10.21608/edj.2018.76780
Osteoconductivity of Two Novel Biodegradable Magnesium Alloys (ZK30A&ZK10A) for Repairing Bone Defect in Dogs
Heba
Shalaby
Associate Professor, Dental Biomaterial Department, Faculty of Dentistry, Alfyoum University, Egypt.
author
Nesrine
khairy
Lecturer of Oral Surgery, Faculty of Dentistry, Cairo University, Egypt.
author
Marwa
Moussa
Lecturer of Oral Biology, Faculty of Dentistry, Cairo University, Egypt.
author
text
article
2018
eng
Background: Magnesium alloys containing zinc and zirconia are promising alloys as biodegradable orthopedic replacement boneThe objective: of this research was to evaluate of the ability of ZK30A and ZK10A Mg-alloys as biodegradable alloplastic bone substitute for bone formation of adequate quality and quantity in repairing bony defects in femoral shaft of dogs. Materials and Methods: A total of eight skeletally mature mongrel male dogs with an average age 20 ± 2-month-old and weighing 15-18 kg were used in this study. The dogs divided into two groups, group I; contains 8 dogs with type I (ZK30A) alloy plates implanted in holes made in upper right femoral shaft and group II contains the same 8 dogs with type II (ZK10A) alloy plates implanted in holes made in lower right femoral shaft. The quantitative and qualitative assessments of newly formed bone tissue were carried out and assessed by In vivo radiography, scanning electron microscope and EDX for elemental analysis.Results: radiographic results of bone density of group I showed high bone resorption after three weeks implantation, followed by high rate of bone formation after 6 weeks, then regressed to bone resorption again, finally after 12 weeks bone deposition again insignificant with those at 6 weeks. Whereas group II showed normal pattern of bone resorption till six weeks, followed by bone formation till the end of experiment. Both types of alloys showed bone resorption first after implantation, but they differed in longevity and severity of initial bone resorption; group I was severe for three weeks whereas group II was less in severity for six weeks.. Scanning electron microscopic results of both magnesium alloys (group I and group II) 3 months post-implantation showed new bone formation. Type I(ZK30A) alloys induced new bone in approximate contact to implanted alloy surface. Whereas, type II (ZK10A) alloys induced new bone in close intimate contact and well integrated with implanted alloy surface EDX for elemental analysis of the newly formed bone showed non-significant difference between group I and group II in calcium, Ca/P ratio and zinc. Conclusion: Both Mg-Zn-Zr alloys are liable to form qualified mineral bone. The pattern of osteointegration is directly related to the biodegradation rate and stable corrosive products formation. Mg Zn alloy with higher Zr (ZK10A) showed lower biodegradation tendency and high susceptibility for new bone integrated with orthopedic implant. Mg Zn alloy with lower Zr (ZK30A) showed higher biodegradation tendency and encouraged new bone formation with wide gap distance. ZK10A is promising osteoconductive biodegradable implant.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2171
2182
https://edj.journals.ekb.eg/article_76782_954b79ca32446dced8eb49289a508ee3.pdf
dx.doi.org/10.21608/edj.2018.76782
Volumetric analysis of total pharyngeal airway space using cone beam computed tomography among adult egyptians
Ahmad
El Rawdy
Lecturer of Oral Radiology, Faculty of Dentistry, Suez Canal University
author
Walaa
Elgemeeay
Lecturer of Orthodontics, Faculty of Dentistry, Suez Canal University
author
text
article
2018
eng
A typical airway is needed for typical improvement of the craniofacial structures. The objective of the research was to; analyse and contrast the pharyngeal airway volume in the class I and class II facial skeletal patterns using cone beam computed tomography. Methods: The present retrospective study was led on 80 cone beam computed tomography (CBCT) records of adult egyptians ranging in age from 20-40 years. The records were equally divided into 2 groups; group I (G1) skeletal class I ANB =1°-4°), (group II, skeletal class II (G2) ANB >4°). Evaluation of the pharyngeal air way space volume was conducted and statistically analyzed to contrast between G1 and G2 Results: Statistically significant distinctions was found on total pharyngea1 airway volume between G1&G2 Conclusion: The entire volume of pharyngea1 airway space was much larger in G1 than G2 subjects.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2183
2189
https://edj.journals.ekb.eg/article_76783_e8ce63f8f6d19701b1b9dca4852d4eb5.pdf
dx.doi.org/10.21608/edj.2018.76783
Chemoprevention efficacy of indole-3-Carbinol in experimentally induced hamster buccal pouch carcinogenesis
Kamal
Kamal
Lecturer of Oral and Dental Pathology - Faculty of Dentistry - Al-Azhar University
author
Magdi
Alazazi
Lecturer of Oral Biology - Faculty of Dentistry - Al-Azhar University
author
text
article
2018
eng
Abstract: The aim of the present study was directed to investigate the effect of indole-3-carbinol (I3C) as a new chemopreventive modality in experimentally induced hamster buccal pouch (HBP) carcinogenesis. Material and methods: Twenty five golden Syrian male hamsters five weeks old, weighting 80-120 gs were used as experimental animals and divided into three group(s) (G(S)) (G1, G2 and G3): G1 (negative control): 5 animals were left untreated. G2: (dimethylbenz[a]anthracene (DMBA) painting-HBP group): 10 animals, their right buccal pouches were painted with 0.5% DMBA in paraffin oil 3 times a week and divided into two subgroups, G2A and G2B (G2A which included 5 animals and painted for 8 weeks, G2B which included 5 animals and painted for 14 weeks. G3 (I3C chemoprevention group): 10 hamsters were included in this group were received I3C given by the oral route using a specific vehicle one week before, as well as during the application of DMPA on alternative days for 8 weeks (G3A) and for 14 weeks (G3B). After termination of the experiment, all animals were sacrificed, and the buccal mucosa was excised and fixed in 10% neutral buffered formalin, routinely processed and embedded in paraffin blocks for preparation in order to be examined histologically and immunohistochemically and then statistical analysis based on these examinations was done Results: Gross observations revealed variable features in (G2 and G3) compared to that observed in group G1ranging from normal and smooth surface to fungating tumor masses of large sizes. Histopathological findings revealed variations among chemoprevention groups ranging from normal epithelial layers to epithelial dysplasia to squamous cell carcinoma with invading nests and pearls. Immunohistochemical (IHC) results, regarding Bax expression, revealed variability in the area percentage throughout the groups used. At 8 weeks, area percentages of G1, G2A, and G3A were (39.83%, 22.00 %, and 30.47 %) respectively, and at 14 weeks were (39.83%, 10.45 % and 26.19%) respectively. Bcl2 expressions also had variability in the area percentage throughout the same groups at 8 weeks were (7.05 %, 43.09 % and 18.15 %) respectively, while at 14 weeks they were (7.05 %, 75.21 %and 43.76 %) respectively. Conclusion: I3C is considered as a promising chemotherapeutic agent in prevention of induced HBP carcinogenesis (epithelial dysplasia & invasive carcinoma) and proved beneficial role in improving the outcome by modulating apoptosis and proliferation throughout the process of carcinogenesis.Keywords: HBP carcinoma, indole-3-carbinol, Bax, Bcl2.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2191
2204
https://edj.journals.ekb.eg/article_76784_274d7f7be9e0d48bb4c0e5766970fad6.pdf
dx.doi.org/10.21608/edj.2018.76784
Intraobserver and Interobserver Reliability Assessment of the Proximity of Maxillary Molars to the Maxillary Sinus Using Cone-beam CT
Sara
El Khateeb
Assistant Professor, Department of Oral Medicine, Periodontology, Diagnosis and Oral Radiology, Faculty of
Dentistry, Ain Shams University, Cairo, Egypt
author
Ebtihal
Zain‑Alabdeen
Lecturer, Department of Oral and Basic Clinical Sciences, College of Dentistry, Taibah University, Al-Madinah
Al-Munawarrah, Kingdom of Saudi Arabia.
author
text
article
2018
eng
Purpose of the study: The aim of the current study was to evaluate the vertical and horizontal relationships between the root apices of maxillary molars and the maxillary sinus floor (MSF). And to examine the Intra- and Interobserver reliability of this assessments.Materials and methods: This retrospective study was conducted at Taibah University Dental College & Hospital. We evaluated the vertical and horizontal relationships between the root apices of maxillary molars and the maxillary sinus floor according to modified classification from Kang et al. 2015 study. Kappa agreement coefficient was calculated to examine the intraobserver and interobserver reliability. Frequency and proportions were tabulated, and Generalized Estimating Equation (GEE) model analysis was performed for the test of significance. Results: The sample contained 216 maxillary molars in total, with 56 missing molars, the analysis was performed on 160 maxillary molars. Kappa agreement coefficient was 0.9857 for intraobserver reliability and were 0.9799 and 0.9803, respectively for interobserver reliability. Concerning vertical measurements, the highest frequency was found in group two (58.8%) where there was close contact between maxillary molar root apices and MSF. For the horizontal relationship, most teeth were in the second group where the MSF is centrally located (37.5%).Conclusion: Our study showed that most of the maxillary molars in our sample had close contact with floor of the maxillary sinus which was centrally located in most of our cases. Also, our study stated that the assessment classification was highly reliable that greatly will assist the clinician to relay on for the assessment and preceding knowledge of relationship between maxillary molars and maxillary sinus floor before any surgical dental procedures which is valuable for preoperative treatment planning and the inhibition of complications.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2205
2215
https://edj.journals.ekb.eg/article_76788_9fcd92370a0df2f6ab068f3ca820ca87.pdf
dx.doi.org/10.21608/edj.2018.76788
Surgical scraping technique for gingival depigmentation: A 6 month clinical study evaluating patient satisfaction and recurrence rate
Ola
Ezzatt
Lecturer of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Ain Shams University
author
text
article
2018
eng
Background: Elimination of gingival pigmentation is a common demand in dental cosmetic. Various methods have been reported in clinical practice. However, simplicity and safety of the technique and maintenance of the results is often a challenge. The aim of this study is to evaluate the clinical effect, patient satisfaction and recurrence rate of pigmentation following surgical scraping technique for gingival depigmentation.Subjects and Methods: This time series prospective clinical study included 15 healthy non-smoker patients seeking gingival depigmentation for esthetic reason. The patients’ sex and age were recorded. Then pigmented gingiva of the whole arch was scraped using Kirkland periodontal knife until the entire visible pigmentation was removed. Dummett-Gupta Oral Pigmentation Index (DOI) was recorded at baseline, 1 month, and 6 months. Patients’ satisfaction and frequency of re-pigmentation were also evaluated.Results: The mean DOI decreased significantly from 2.53 (0.52) at baseline to 0.60 (0.51) after 1 month and the 6 month score was 0.93 (0.59). By the end of 1 month the percentage of cases that showed any sign of pigmentation recurrence was 13.3% and 33.3% by the end of 6 months, 10 patients (66.6%) had persistence results and no cases returned to baseline score. (86.7%) experienced no pain during the surgical procedure and only (33.3%) had severe pain at the day of surgery and all patients were pain free in the first week. (80%) reported marked cosmetic changes by the end of 1st week and 6 months.Conclusion: The surgical scarping gingival depigmentation technique produced desired and persistence clinical results up to 6 months and all patients were satisfied with the procedure and cosmetic outcomes.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2217
2224
https://edj.journals.ekb.eg/article_76789_eb46c988fd09c88729fef3ac65885576.pdf
dx.doi.org/10.21608/edj.2018.76789
Radiographic Evaluation of Periodontal Tissue Detected in Cone Beam CT Images for Teeth with and Without Apical Periodontitis A Retrospective Study
Abdulkarim
AlShehri
Periodontist Specialist, Ministry of Health, Kingdom of Saudi Arabia
author
Sami
Shafik
Professors of Periodontics Riyadh Elm University
author
Hafez
Diab
Professor of Periodontology, Tanta University
author
text
article
2018
eng
Aim of the study: The aim of the present work was to evaluate the alveolar bone level and periodontal ligament space thickness of teeth that have apical periodontitis and compare it with teeth without apical periodontitis using cone beam CT images.Materials & Methods: The study design was a retrospective cross sectional epidemiological study for evaluation of periodontal tissue of 100 teeth using cone beam CT images. The sample consisted of CBCT images of the maxillofacial region from database of 50 patients which were selected from Riyadh Colleges of Dentistry and Pharmacy. The 100 CBCT images were analyzed for the following variables 1-Periodontal ligament space of buccal surfaces at 3 sites cervical, middle and apical third. 2- Periodontal ligament space of lingual surfaces at 3 sites cervical, middle and apical third. 3-Alveolar bone level: the distance from CEJ to the crest of alveolar bone mesially and distally. These variables were detected on teeth with periapical periodontitis and compared with same teeth in the contralateral side of the same arch.Results: The buccal and lingual periodontal ligament space thickness in cervical, middle, and apical third of teeth with periapical lesion was statistically significant higher than the contra lateral teeth without periapical lesion of the same arch (p<0.05). Furthermore, the alveolar bone measures from CEJ to alveolar crest in both mesial and distal sides of the teeth with periapical lesion was statistically significant higher than the contralateral teeth without periapical lesion of the same arch (p<0.05).Conclusions: The present study showed that, periapical infection has negative effects on alveolar bone level and periodontal ligament space thickness. CBCT is an effective diagnostic method to visualize and measure the periodontal ligament space and alveolar bone level.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2225
2230
https://edj.journals.ekb.eg/article_76790_3050ae8ae660beee9320bdd85914a950.pdf
dx.doi.org/10.21608/edj.2018.76790
Impact of cod liver oil supplementation on the histological changes of the tongue dorsal surface in insulin treated streptozotocin induced diabetic rats
Heba
El-Imam
Lecturer of Oral Biology, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt
author
Nesreen
Abdel-Salam
Lecturer of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt
author
text
article
2018
eng
Background: Diabetes mellitus (DM) is one of the most common chronic metabolic disease that affecting all age. However, it has many complications such as stomatitis, delayed wound healing, mucosal neuro-sensory disorders. Cod liver oil (CLO) is an important source of ω-3 fatty acids that have anti-inflammatory effect. Objective: The present study designed to investigate the protective effect of cod liver oil (CLO) supplementation in ameliorating the histological changes of the tongue dorsal surface in streptozotocin (STZ) induced diabetic rats. Design: Forty adult male Swiss albino rats were randomly divided into four groups (n=10). Group (I) rats were received single intraperitoneal injection of 1 ml/kg citrate buffer. Group (II) rats were received single intraperitoneal injection of 60mg/kg STZ freshly dissolved in 1 ml/kg citrate buffer, blood samples were obtained and fasting glycaemia was measured to confirm the development of diabetes. Group (III) after confirmation of diabetes rats were received subcutaneous injection of human insulin with a dose (5 IU/kg/d). Group (IV) rats were treated as in group (III) and received pure cod liver oil with a dose (60mg/Kg /d) by intra-gastric intubation. After four weeks, animals were scarified and tongues were dissected and the prepared sections were examined histologically by H&E, histochemically by Masson Trichrome stain (MTC) and morphomtrical analysis. Data obtained from morphomtrical analysis were statistically described in terms of mean ± standard deviation (± SD). Results: Histological examination of Group I revealed the normal features of the dorsal surface of tongue and shape of filiform papillae. In Group II has evident hyperkeratosis and loss of conical shape of filiform papillae. Group (III) has better epithelium and keratin layer and shape of filiform papillae. Group IV has histological features resembling nearly those of group I. The morphometric analysis confirmed the previous results as group I showed the highest mean epithelium thickness, followed by Group IV, then Group III and the least value was for Group II. Conclusions: diabetes has a deleterious effect on dorsal surface of the tongue. Insulin can’t completely inhibit the complications of diabetes. However, ω-3 fatty acids present in CLO has protective effect on these abnormalities caused by diabetes.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2231
2240
https://edj.journals.ekb.eg/article_76791_e437f36deeffa2efcfeb89913fff5e6c.pdf
dx.doi.org/10.21608/edj.2018.76791
Evaluation of the effect of Tartrazine versus curcumin as food coloring agent on tongue papillae of albino rats (histological and scanning electron microscopic study)
Heba
El Imam
Lecturer of Oral Biology, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt
author
Nesreen
abd El Salam
Lecturer of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
author
text
article
2018
eng
Introduction: Color additives are used in food for improving the aesthetic appearance. They have been caused many health problems. Tartrazine is widely used food color additives and considered as food carcinogen. Curcumine has been found to be used as a promising natural food additive. Aim: investigate the effect of synthetic coloring additive tartrazine and natural one curcumin (has the same yellow color) on two main types of lingual tongue papillae, the filiform and fungiform papillae of albino rat. Materials and Methods: Fifteen adult male albino rats (180 -200 gm) divided randomly into three groups. (n=5). Group I (Control): rats were given 1 ml distilled water. Group II (Tartrazine-treated): rats were given Tartrzine dissolved in distilled water with dose of 7.5 mg/kg. Group III (Curcumine- treated): rats were given 200mg\kg of curcumin suspended in 0.5% carboxymethylcellulose (CMC). All of them gave the dose once daily by oral intubation. After 30 days of treatment, animals were scarified and tongues were dissected and cut longitudinally into two half one processed for light and the other for scanning electron microscopic examination SEM. Results: Histological examination of group I revealed the normal features of the dorsal surface of tongue and shape of filiform papillae. In group II has distortion of filiform and fungiform papillae and evidence of acanthosis, hyperkeratosis, and the basal cell layer has basilar hyperplasia, nuclear hyperchromatism. Disfigured fungiform papillae with swollen taste buds and its cells appeared separated. However, group III filiform and fungiform papillae appeared close to normal with minimal changes. Conclusion: From the present study we concluded that tartrazine has degenerative changes in both the filiform and fungiform papillae with absence of these degenerative effects in comparison with curcumin. Replacement of synthetic additives by natural food ones help to avoid the adverse effects of synthetic one on lingual tongue papillae.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2241
2250
https://edj.journals.ekb.eg/article_76792_d42842cf0a6af480ff5fc3a7ceb1bb2e.pdf
dx.doi.org/10.21608/edj.2018.76792
ABO Blood Grouping from Dentin and Pulp of Fresh and Aged Teeth by Modified Absorption–Elution Technique
Shimaa
Motawei
Lecturer of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Mansoura University, Egypt.
author
Rehab
El-Zehary
Assistant Professor of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt.
author
Ahmed
Shteiwi
Dentist in the Ministry of Health and Population, Egypt
author
text
article
2018
eng
Background : Teeth are one of the hardest, most stable and most durable structures in the body. They are also easily accessible for examination. Therefore, teeth are a first-rate material for forensic investigations.Objectives: This study was carried out to test the possibility of detection of ABO blood groups through examination of the pulp of teeth as a soft tissue and the dentin as a hard tissue, and also to evaluate the reliability of teeth stored for a relatively long period of time as a source for blood group identification by absorption–elution technique with some modifications. Materials & Methods: Fifty-two sound human teeth were examined for the ABO blood groups in both dentin and pulp tissues for both fresh and aged teeth, and results were compared with the blood groups obtained by testing a blood sample of the same individual using the simple slide test. Teeth were split using a new Hand-held pulp Isolator instrument. Results: The teeth pulp showed a very highly positive correlation in both fresh and aged teeth though it decreased slightly in the latter. Dentin showed a positive correlation in both fresh and aged teeth groups indicating that the dentin as the hard tissue of the teeth is quite reliable to detect blood groups. Effects of the age, sex, and jaw distribution on the blood grouping from the teeth were also carried out. Conclusion: The hard and soft tissues of teeth are reliable sources for blood group determination and help in human identification. Sensitivity of the ABO antigens detection in dental material is higher in the dental pulp than the dental hard tissue, and it decreases with the lapse of time. Modified absorption elution method is accurate in that respect. Introduction of the new Hand-held pulp Isolator instrument provides more teeth material to be tested, hence more accurate.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2251
2261
https://edj.journals.ekb.eg/article_76793_2a818ab550c5c5b5771eb1362e1437b1.pdf
dx.doi.org/10.21608/edj.2018.76793
Piezoelectric-Alveolar Ridge Splitting: A Successful Modality Reducing Crestal Bone Loss around Dental Implants and Enhancing Implant Secondary Stability
Ahmed
Amr
Lecturer of Oral Medicine and Periodontology, Faculty of Dentistry, Ain Shams University
author
Amr
Ghanem
Lecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ain Shams University
author
text
article
2018
eng
Aim: This study was performed to compare two different modalities of alveolar ridge splitting performed simultaneously with dental implant placement regarding the alveolar crestal bone changes and implant stability Subjects and methods: 12 patients were included in this study. Group 1 (6 patients, 12 implant) while Group 2 (6 patients, 10 implants). Single surgery was performed to each patient involving alveolar ridge splitting, expansion and simultaneous implant (or implants) placement. Ridge splitting in group 1 was performed using the piezoelectric surgery and in group 2 using the traditional surgical disc, while the alveolar ridge expansion and implant placement were performed using the same procedures for both groups. Marginal bone loss on the buccal and lingual aspects of the implant was evaluated 4 months after implant insertion using the CBCT. The implant stability ISQ values were measured immediately after implant insertion and 4 months after the surgery during the prosthetic steps by the Ostell. Results: Post treatment, a higher mean percent resorption in marginal bone was recorded in traditional surgical disc group with an extremely significant difference between groups. Also, a higher mean percent increase in ISQ2 was recorded in piezo- electric surgery group with an extremely significant difference between groups. Conclusion: Piezoelectric ridge splitting is accompanied with decreased postoperative marginal bone loss and enhanced secondary ISQ compared to traditional surgical disc splitting.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2263
2272
https://edj.journals.ekb.eg/article_76797_d69d677a0b7178a005587e8ac349af9a.pdf
dx.doi.org/10.21608/edj.2018.76797
Osseointegration of Implant Placed in Extraction Socket and Augmented with Alloplastic Bone Substitutes and Subepithelial Connective Tissue Graft in Comparison to Those Augmented with Alloplastic Bone Substitutes Only (A Randomized Controlled Clinical Trial)
Yasser
El-Makaky
Assistant Professor of Oral Medicine, Periodontology, Oral Diagnosis & Radiology Department, Faculty of Dentistry, Tanta University, Egypt
author
text
article
2018
eng
Background: The objective of this study was to assess the osseointegration around endosseous dental implants inserted in fresh extraction sockets using β-Tricalcium phosphate bone graft with and without subepithelial connective tissue graft.Methods: Twenty patients were enrolled in this study and allocated randomly to control group (Ten fresh extraction sockets were implanted by immediate dental implants and grafted by ß-tricalcium phosphate) or test group (Ten fresh extraction sockets were implanted by immediate dental implants and grafted by ß- tricalcium phosphate with a subepithelial connective tissue graft). The clinical parameters were monitored at 6,9 and 12 months post-implantation while the radiographic evaluation was conducted immediately after implant insertion(baseline),6 and 12 months postoperative. The implant stability was monitored using periotest at baseline,6 and 12 months after implantation. Results: All implants in both groups were well osseointegrated resulting in a 100% cumulative success rate after one year follow up. There were no significant differences between the studied groups regarding clinical and radiographically parameters throughout the study period. However, the patients in test groups displayed better assessments regarding pocket depth, periotest value, keratinized mucosa and marginal bone level than patients within the control group.Conclusion: The using of subepithelial connective tissue graft as a membrane with β-Tricalcium phosphate bone graft could improve osseointegration around dental implants in fresh extraction sockets.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2273
2286
https://edj.journals.ekb.eg/article_76799_11e5da569440e7b58fba29392cacf345.pdf
dx.doi.org/10.21608/edj.2018.76799
Effect of Atorvastatin and Remifemin on Glucocorticoid Induced Osteoporosis in Rats with Experimental Periodontitis. A Comparative Study
Heba
Shawky
Associate Professor of Oral Medicine and Periodontology, Oral Medicine and Periodontics Department, Faculty
of Dentistry, Pharos University in Alexandria, Egypt.
author
Marwa
Essawy
Fellow Lecturer of Oral Pathology, Oral Pathology Department, Faculty of Dentistry, Alexandria University,
author
text
article
2018
eng
Periodontitis is a progressive destructive disease affecting the tooth supporting structures. Osteoporosis can enhance the rate and severity of periodontal destruction. In osteoporosis, lowering of the estrogen level leads to imbalance between bone formation and resorption. Different drugs were utilized to manage osteoporosis. Statins are cholesterol lowering drugs. Different studies have shown that statins have an anti-resorptive effect. Furthermore, Remifemin is a herbal drug that demonstrated certain favorable effects on bone integrity. The present study was performed to compare the impact of Atorvastatin and Remifemin in osteoporotic rats with induced periodontitis. Materials and Methods: Fifty male albino rats weighing 300 ± 50 gm were enrolled in the current study. The rat population was divided into five groups, ten rats each: control group (Group I), Group II with ligature induced periodontitis, Group III with induced periodontitis and osteoporosis, the Atorvastatin-treated group (Group IV) and finally the Remifemin-treated group (Group V). Rats were sacrificed after five weeks. Histological and histomorphometric examinations were performed. Results: Alveolar bone in Group V exhibited more bone formation with regular bone surface compared to Group IV. Histomorphometric results revealed that both treated groups showed substantial improvement in bone volume and in osteoblast count compared to Group II and III. However better results were noted in Group V. Conclusion: Superior results were demonstrated with Remifemin concerning bone volume. The authors suggested utilization of Remifemin as a beneficial therapeutic drug.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2287
2296
https://edj.journals.ekb.eg/article_76800_d317077f146e8bfd7151736f12581a72.pdf
dx.doi.org/10.21608/edj.2018.76800
Immunohistochemical Evaluation of Bcl-2 and Ki-67 in Buccal Mucosa of Induced- Immunosuppressed Albino Rats
Enas
Hegazy
Lecturer of Oral Biology,Oral Biology Department, Faculty of Dentistry, Suez Canal University.
author
Merhan
Elmansy
Lecturer of Oral Pathology ,Oral Bpathology Department, Faculty of Dentistry, Suez Canal University.
author
Elham
Mahmoud
Associate Professor, Oral Biology Department, Faculty of Dentistry, Suez Canal University, Egypt.
author
text
article
2018
eng
Introduction: Organ transplantation provides life-saving therapy for patients with end-organ disease. Tacrolimus is an immunosuppressive drug used to lower the risk of organ rejection. This study was performed to investigate the immunohistochemical expressions of Ki-67 (a proliferation associated antigen) and Bcl-2 (antiapoptotic protein) and their correlation with the increased risk of development of neoplasms in the buccal mucosa of tacrolimus-induced immunosuppressed albino rats. Material & methods: Twenty adult male albino rats were divided into two equal groups. Group one treated with tacrolimus in a daily oral dose 0.5 mg/kg for 3 months. Group two served as negative control, received distilled water in comparable volume. Tissue samples were fixed in 10% formalin, embedded in paraffin, and stained with hematoxylin and eosin to evaluate histopathological finding. Other sections were used to reveal the immunohistochemical expression of Ki-67 and Bcl-2 antibodies through computerized image j analysis software. The expression of both markers was analyzed statistically through using Mann-Whitney test. Results: Histopathological finding showed moderate dysplasia in tacrolimus-treated group. There was marked increase in normal mitotic figures, basal cell hyperplasia, loss of basal cell polarity, swirling of the spinous layer, prominent nucleoli, hyperchromatism, and altered N/C ratio. Immunohistochemical finding revealed marked increase in the nuclear expression of Ki-67 in basal cells and cytoplasmic expression of Bcl-2 in all layers of keratinocytes in tacrolimus-treated group. Statistical finding in tacrolimus – treated group showed significant difference in the expression of Ki-67 and highly significant difference in the expression of Bcl-2. Conclusion: There is an obvious relation between prolonged systemic use of tacrolimus and development of premalignant lesions. The marked increase in Ki-67 and Bcl-2 expression may play a role in tumorgenesis.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2297
2304
https://edj.journals.ekb.eg/article_76801_af1d7c9ab7418e543c206ea7fac098fe.pdf
dx.doi.org/10.21608/edj.2018.76801
Effect of fish oil supplementation on alveolar bone structure in rats with glucocorticoid induced osteoporosis (Histological, immunohistochemical & ultrastructural study)
Dina
Nagui
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.
author
Nesma
Khalil
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.
author
text
article
2018
eng
Glucocorticoids are widely used in treatment of many medical disorders. However, many side effects are associated with the chronic use of glucocorticoids administration especially osteoporosis. Fish oil contains omega 3 polyunsaturated fatty acids which can inhibit the production of inflammatory mediators such as IL-6 and TNF-α which are important for osteoclastogenesis. So the aim of the present work was to test the efficiency of fish oil administration on alveolar bone osteoporosis induced by glucocorticoid administration. Materials & methods: 8 Wistar albino rats were divided into 3 groups. Group Ι: control group. Group ΙΙ (osteoporotic group): rats were given 7 mg/kg of dexamethasone, once a week intramuscularly for 5 weeks. Group ΙΙΙ (fish oil treated group): rats were given the same dose of dexamethasone like group ΙΙ and at the same time were given daily an oral dose of fish oil 1gm/kg/day. All rats were sacrificed after 5 weeks from the start of the experiment and molar segments of the jaw were dissected out. They were examined histologically, immunohistochemically, histomorphometrically and ultrastructurally. Results: Histologically in osteoporotic group ΙΙ the bone showed marked resorption with the presence of many osteoclasts which showed significant increase in number and intense TRAP immunostaining. By scanning electron microscope the buccal cortical plate showed obvious erosion. On the other hand, fish oil treated group showed marked improvement where the bone appeared denser with significant decrease in osteoclast number which exhibited mild TRAP staining. Histomorphometriclly, the bone surface area showed marked increase. Ultrastructurally, the bone surface appeared relatively smooth. Conclusions: fish oil supplementation could to a large extent overcome the detrimental effect of glucocorticoid administration on the structure of the alveolar bone.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2305
2317
https://edj.journals.ekb.eg/article_76803_0ecee89de0dd3d94b6945837322bf844.pdf
dx.doi.org/10.21608/edj.2018.76803
PROBIOTICS EFFECT ON THE GINGIVA OF UNCONTROLLED DIABETIC ALBINO RATS
Samah
Kamel
Lecturer Oral Biology, Faculty of Dentistry, October University of Modern Sciences and Art
author
Mohamed
ElKady
Lecturer Oral Biology, Faculty of Dentistry, October University of Modern Sciences and Art
author
text
article
2018
eng
Objective: This study was designed to evaluate the effect of using L.Reuteri containing tablets, on the gingiva of uncontrolled diabetic rats both histologically and ultrastructurally.
Material and Methods: Thirty adult male albino rats were equally divided into three groups, Group I served as control, Group II: rats were subjected to induction of diabetes mellitus using streptozocin, Group III: rats treated as group II animals and on the next day they were fed daily a probiotic tablet Prodentis containing L. reuteri for 14 days. At the end of the experiment, histological and ultrastructural examination was performed on the gingiva.
Results: Rats of group II revealed inflammatory gingiva with loss of normal structure, while that of group III revealed a somewhat normal gingiva in comparison to group II.
Conclusion: L.reuteri containg tablets can inhibit gingival inflammation associated with diabetics through its antibacterial and autoinflammatory properties.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2319
2325
https://edj.journals.ekb.eg/article_78462_d1c666cc486ebfb62e4850d3a806db90.pdf
dx.doi.org/10.21608/edj.2018.78462
Accuracy of QuickScan Imaging Protocols of iCAT FLX CBCT in Assessment of Peri-implant Bone Defects
Wael
Aboelmaaty
Lecturer of Oral Radiology and Diagnosis, Faculty of Dentistry, Mansoura University
author
Nermin
Morgan
Assistant Lecturer of Oral Radiology and Diagnosis, Faculty of Dentistry, Mansoura University
author
Ehab
Abdelfadil
Lecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry, Mansoura University
author
Mostafa
Ashmawy
Lecturer of Oral Radiology, Oral Radiology Department, Faculty of Dentistry, Ain-Shams University
author
text
article
2018
eng
Aim of the study: The current study was conducted to determine the accuracy of QuickScan imaging protocols of iCAT Flx CBCT machine in detection and measuring crestal bone defects around dental implants.Materials and Methods: 20 simulated peri-implant crestal bone defects were created in bovine ribs bone models then were scanned by iCAT FLX CBCT machine with HD Scan 0.25 mm voxel, QuickScan 0.25mm voxel, and QuickScan + 0.3mm voxel sizes. All scans were imported in on-demand 3D App software and produced linear measurements were compared to true measurements done directly with digital caliper on bone models.Results: All imaging protocols of CBCT showed no statistical significance in detection of shallow peri-implant bone defects while the QuickScan + imaging protocol showed less accuracy in measuring the depth of defects.Conclusion: QuickScan + can be used as a low dose periodic follow up imaging utility for postoperative implant surgery for detection of any small bone changes but if more details are needed QuickScan technique is required.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2327
2336
https://edj.journals.ekb.eg/article_76810_9b2e368fcae78d9f65ce5f4b58cd60f8.pdf
dx.doi.org/10.21608/edj.2018.76810
Evaluation of the therapeutic potential of Tamarind seeds (Aqueous extract) versus antidiabetic drugs on the histological structure of lingual papillae in diabetic rats
Rehab
Abdel Moneim
Associate Professor, Oral Biology Department, Faculty of Dentistry, Cairo University and Future University in Egypt.
author
Mona
El Deeb
Associate Professor, Oral Biology Department, Faculty of dentistry, Future University in Egypt.
author
Fatma
Adel
Lecturer, Oral Biology Department, Faculty of dentistry, Future University in Egypt
author
text
article
2018
eng
Objective: Diabetic patients suffer from several oral complications; thus there has been unending efforts in searching for treatment agents either in synthetic forms or from plant sources. The aim of the present study is to prove the efficacy of aqueous tamarind seeds extract in treatment of the complications that might occur in tongue papillae of high fat diet/streptozotocin (HF/STZ) type 2 diabetic rats relative to the conventional anti-diabetic drugs. Design: Thirty five adult male albino rats (200-220 gm) were selected for this study. The animals were randomly divided into five groups (seven rats each). Group I (Control –ve), Group II (Diabetic control): Type 2 diabetes was induced by 58% calories HF diet for 4 weeks followed by intraperitoneal administration of STZ (35 mg/ kg). Two weeks after STZ injection, diabetic rats were treated with oral doses of antidiabetic drugs as follows: Group III: Metformin (500mg/1 ml distilled water/ kg/twice daily), Group IV: Forxiga (0.1 mg/1 ml distilled water/ kg/day) and Group V: Aqueous tamarind seeds extract (80 mg/0.5 ml distilled water/100 g/day) for 4 weeks. Blood glucose level was measured every week. At the end of the experiment, tongue specimens were dissected and processed for light and environmental scanning electron microscopic examination. Results: Histological and ultrastructural examination of Group II (Diabetic group) revealed signs of deterioration and degeneration among the filiform and fungiform papillae. After 4 weeks treatment minor to moderate improvement in the architecture of the papillae has been reported in Groups III and IV respectively. In comparison to Groups III and IV, oral administration of tamarind seeds extract (Group V) caused significant improvement in the histological structure of the lingual papillae, covering epithelium, their taste buds and the associated gustatory pore. Conclusion: The findings of the present study indicate that aqueous extract of tamarind seeds possessed significant anti-diabetic and anti-inflammatory activity for improvement of diabetes associated oral complications that may prove its beneficial potentiality in treatment of type 2 diabetes.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2337
2354
https://edj.journals.ekb.eg/article_76811_2f088425e6f546a8c2ccfac401b352d9.pdf
dx.doi.org/10.21608/edj.2018.76811
Cone Beam Computed Tomography role in surgical treatment planning of Obstructive Sleep Apnea patients
Wael
Aboelmaaty
Lecturer of Oral and Maxillofacial Radiology, Faculty of Dentistry, Mansoura University, Egypt.
author
Marco
Isaac
Faculty of Oral & Dental Medicine, Delta University for Science & Technology, Egypt.
author
A.
Elsobky
Lecturer of E.N.T, Faculty of Medicine, Mansoura University, Egypt.
author
M.
Elewa
Professor of Oral Medicine and Periodontology, Faculty of Dentistry, Mansoura University, Egypt.
author
text
article
2018
eng
Purpose: To evaluate the accuracy of cone beam computed tomography (CBCT) as a diagnostic tool that can be used (alone or in conjunction with another diagnostic tool) in surgical treatment planning of obstructive sleep apnea (OSA) patients and to determine level of collapse in the upper airway.Methods: Using a case-control study, 40 patients subjected to upper airway surgery were selected in this study. All patients were subjected to physical, clinical evaluation and Drug Induced Sleep Endoscopy (DISE), for 20 patients surgery was done without CBCT scanning, while 20 patients surgery was done after CBCT scanning and assessing radiographic findings including virtual endoscopy with clinical findings. Both groups were clinically evaluated after six months of the surgery to determine surgical treatment planning success. For all tests P≤0.05 was considered statistically significant.Results: Group II was more superior over group I in postoperative clinical parameters such as patient satisfaction and AHI reduction. Conclusion: CBCT is a low dose highly efficient diagnostic tool that is very important in proper evaluation of OSA. Virtual endoscopy is a noninvasive impressive tool for 3D soft tissue simulation.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2355
2360
https://edj.journals.ekb.eg/article_76812_998f8062e7bc220010bf55ad67db700d.pdf
dx.doi.org/10.21608/edj.2018.76812
Clinical evaluation of Ribes nigrum and Vaccinium myrtillus extract on the healing of gingivectomy wounds
Gehan
Kotry
Associate professor, Department of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of
dentistry, Alexandria University, Egypt
author
Maha
Taalab
Lecturer, Department of Oral Medicine, Periodontology, Oral Diagnosis and Radiology, Faculty of dentistry,
Alexandria University, Egypt
author
text
article
2018
eng
Background : Wound healing following gingivectomy is a complex process that is affected by a variety of factors. Whenever possible, rapid healing and a pain –free postoperative period are desirable after any surgical procedure. A topical gel containing Ribes nigrum and Vaccinium myrtillus extracts (Vitrocure®) was found to possess antiseptic, anti-oxidant and anti-inflammatory effects. Aim: The current study attempted to evaluate the efficacy of this gel on postoperative wound healing and pain control after gingivectomy. Material and methods: The study included 20 gingivectomy cases divided into : Group-1 (Test group): comprised 10 cases where the gingivectomy sites were covered by the gel and cyanoacrylate dressing, post operatively. Group-2 (Control group) : comprised 10 cases that were covered by cyanoacrylate only. Healing was assessed using the healing index of Laundry. Pain was assessed using a numerical pain index . Results: The test group demonstrated superior healing when compared to control at one and two weeks, postoperatively. Moreover, patients reported less postoperative pain in the first five days after surgery in comparison to control group.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2361
2369
https://edj.journals.ekb.eg/article_76813_7130a79fc228141ec8d5c232d729b497.pdf
dx.doi.org/10.21608/edj.2018.76813
COMPARISON BETWEEN SOLUTION AND GEL FORMS OF THEOBROMINE AND SODIUM FLUORIDE IN REMINERALIZATION OF THE DEMINERALIZED HUMAN ENAMEL (SEM and EDXA study)
Safaa
Hussein
Lecturer, Department of Oral Biology, Faculty of Oral and Dental Medicine, Ain Shams University.
author
Khaled
El-Haddad
Lecturer, Department of Oral Biology, Faculty of Oral and Dental Medicine, Ain Shams University.
author
text
article
2018
eng
Introduction: Enamel demineralization occurs by acid attack during dental caries. The initial caries could be arrested by enhancement of remineralization via topical fluoride application. Although fluoride is effective in enamel protection, the safety of its use is a matter of controversy which necessitated the search for new alternatives. Theobromine is one of the promising materials in caries prevention which is safer than fluoride. The effect of the remineralizing material consistency “gel or solution” is essential to be evaluated to reach the optimum protocol.Purpose: To compare between the gel and solution forms of both theobromine and fluoride in the remineralization potential of the demineralized human enamel. Materials & Methods: Thirty longitudinal halves of human mandibular premolars were equally divided into 6 groups: control (C), demineralization (D) subjected to demineralizing solution then 5 days PH cycle. The remaining 4 groups were similar to demineralization group with addition of treatment material “theobromine solution (T1), theobromine gel (T2), fluoride solution (F1) and fluoride gel (F2)”. The samples were investigated by scanning electron microscope (SEM) and energy dispersive x ray analysis (EDXA). Results: The enamel of demineralization group was porous with erosive changes exposing the subsurface enamel rods with severe rod core defects. Theobromine solution and fluoride gel groups showed improvement of the enamel surface. Fluoride solution group showed more observable enamel defects while the least favorable enamel topography was observed in the theobromine gel group. EDXA revealed that the calcium phosphorus ratio displayed a descending order: (C > T1 > F2 > F1 > T2 > D). Conclusion: Theobromine solution and fluoride gel are more effective remineralizing agents. Fluoride solution and theobromine gel have less remineralizing potential.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2371
2380
https://edj.journals.ekb.eg/article_76814_1159ced28be60aad6c769cb689726959.pdf
dx.doi.org/10.21608/edj.2018.76814
Enamel Surface Alterations in Molars of Young Rats of Diabetic Mothers
Tahany
Haggag
Associate Professor, Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Egypt
author
Elham
Mahmoud
Associate Professor, Department of Oral Biology, Faculty of Oral and Dental Medicine, Suez Canal University, Egypt
author
text
article
2018
eng
Objective: Enamel defects are remarkable problems frequently observed in offspring of diabetic women. So, the goal of the current work was determination of enamel surface alterations in molars of young rats born to diabetic mothers.Material and Methods: 16 heathy adult female albino rats were equally divided into two groups. Induction of diabetes was done in one group by alloxan and the other group act as control group. Four adult male rats were used for fertilization of both groups. All off springs were sacrificed with an overdose of ether at 6 weeks after birth. SEM examination of the occlusal surfaces of the mandibular first molar teeth was done using environmental scanning electron microscope. Teeth were examined for chemical characterization using Electron Dispersive Analytical X-ray (EDAX).Results: Enamel revealed multiple ultrastructural alterations in diabetic group. By EDAX examination, there was a decrease in the content of calcium and phosphorous in diabetic group as compared with control, altering the calcium to phosphorus ratio.Conclusion: Maternal diabetes had a detrimental influence on the function of ameloblasts in laying down enamel inducing defective enamel.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2381
2389
https://edj.journals.ekb.eg/article_76815_328fe33c2e4a379edff963b42e30a349.pdf
dx.doi.org/10.21608/edj.2018.76815
Salivary Levels of α-Amylase & Cortisol In Patients With Recurrent Aphthous Ulceration
Gihane
Madkour
Associate Professor of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, Cairo University, Egypt.
author
Ibrahim
El Refaie
Lecturer of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, Cairo University, Egypt.
author
text
article
2018
eng
Objective: Psychological stress is one of the prime triggering factors for recurrent aphthous ulceration. The goal of this investigation is to measure salivary levels of α-Amylase and Cortisol, as stress biomarkers, in patients with recurrent aphthous ulceration. Subjects and Methods: Whole unstimulated salivary samples were collected from 25 patients with recurrent aphthous ulceration & 25 healthy controls. Salivary levels of α-Amylase and Cortisol was quantified using enzyme-linked immunosorbent assay (ELISA). Results: A statistically significant increase in salivary levels of both α-Amylase and Cortisol was detected in recurrent aphthous ulceration patients compared to healthy controls.Conclusion: Salivary α-Amylase and Cortisol can be used as reliable stress biomarkers in patients with recurrent aphthous ulceration.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2391
2397
https://edj.journals.ekb.eg/article_76816_e5783b8f16bce25a731d0fdf10560edd.pdf
dx.doi.org/10.21608/edj.2018.76816
LEVELS OF VITAMIN D AND CALCIUM AS RISK FACTORS FOR CHRONIC PERIODONTITIS
Sandy
Hassan
Lecturer in Department of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Dentistry, Fayoum University
author
Nayroz
Tarrad
Lecturer in Department of Oral Medicine, Periodontology and Oral Diagnosis, Faculty of Dentistry, Fayoum University
author
Olfat
Shaker
Professor in Department of Medical Biochemistry, Faculty of Medicine, Cairo University
author
text
article
2018
eng
Objectives: This study was conducted to estimate, compare and correlate the serum and salivary vitamin D3 (VitD3) in form of [25 (OH) D] and calcium (Ca) levels in healthy subjects and chronic periodontitis (C.P) patients.
Subjects and methods: The study included 50 subjects divided into 2 groups: 30 C.P patients and 20 periodontally healthy subjects. Clinical examination was performed for all subjects, salivary and blood samples were collected from all 50 subjects. Salivary and serum VitD3 levels were assessed by ELISA assay, while Ca was measured colouremetrically.
Results : Salivary and serum VitD3 levels were significantly higher in control subjects compared to C.P patients. Salivary Ca level was significantly higher in C.P group than control group but serum Ca level showed insignificance higher level in control group than C.P patients. No significance correlation was observed except for saliva and serum VitD3 levels there was a statistical significance correlation in the control group.
Conclusion: Low salivary and serum VitD3 and high salivary Ca levels are associated with periodontitis, they might be considered as risk factors for periodontal diseases but this association need to be confirmed in more future studies.
Egyptian Dental Journal
The Egyptian Dental Association (EDA)
0070-9484
64
v.
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
no.
2018
2399
2407
https://edj.journals.ekb.eg/article_78467_b47810f1089adc601d5073045315e125.pdf
dx.doi.org/10.21608/edj.2018.78467