@article { author = {Soliman, Omar}, title = {TREATMENT OUTCOME OF DRY SOCKET FOLLOWING IMMEDIATE IMPLANT PLACEMENT USING PLATELET RICH FIBRIN: A PROSPECTIVE MULTI-CENTRE CLINICAL STUDY}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2101-2108}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.62600.1497}, abstract = {Abstract: Objectives: Dry socket is a common post-extraction complication. It is possible to occur following immediate implant placement. This study was designed to assess the efficacy of PRF for the management of established dry socket following immediate implant placement. Materials and Methods: Twenty patients with established dry sockets following immediate implant placement were included. Patients were divided randomly, patients of group I were treated by socket curettage and irrigation, while in group II PRF was inserted in the space around the implant and filled the socket. Pain and granulation tissue formation score has been used to assess both groups. Results: The pain score was 6 to 10 on the day zero, 3 to 6 on the day four and 1 to 3 on the day seven in group I, while in group II the pain score was 6 to 10 on the day zero, 0 to 3 on the day four and 0 to 1 on the day seven (p 0.001). On group II the formations of granulation tissue appeared earlier than group I. Four implants failed, three implants in group I and one implant in group II. Conclusion: This study suggests that the use of PRF for the management of dry socket following immediate implant placement relieves pain, accelerates the process of wound healing and increases implant success rate.}, keywords = {Dry socket,Immediate implant placement,PRF}, url = {https://edj.journals.ekb.eg/article_182112.html}, eprint = {https://edj.journals.ekb.eg/article_182112_2a22c5d26f619543c9bb801ab864fc36.pdf} } @article { author = {Deraz, Elsayed and Soliman, Omar and Saleh, Reda}, title = {Evaluation of Gingival Tissue Pathological Changes in Human Peri-implantitis: Histological, Immunohistochemical and Ultrastructural Study}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2109-2119}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.66411.1541}, abstract = {Aims: The current research was performed to study histopathological, immunohistochemical and ultrastructural characteristics of gingival tissue in peri-implantitis. Methods & Results: This study examined 25 patients between ages of 30–40 years. Twenty patients were selected with peri-implantitis (peri-implantitis group) in which inflamed gingival tissues were surgically removed. The gingival soft tissue collars from five healthy patients with partially impacted third molar were surgically removed (control group). All gingival tissue samples were examined histologically and ultrastructurally. Immunoreactivity to CD3 and CD20 antibodies was also evaluated. Cases of peri-implantitis stained with hematoxylin and eosin demonstrated hyperplastic nonkeratinized epithelium with downward growth of rete ridges. Connective tissue stroma revealed prominent vascularity, proliferation of fibrous tissue and focally aggregated inflammatory cell infiltration localized to the subepithelial area. Immunohistochemical results exhibited large numbers of T (CD3+) and B (CD20+) lymphocytes in peri-implantitis compared to normal tissue with a statistically significant difference. All cases of peri-implantitis demonstrated no significant difference between T and B lymphocytes. Transmission electron microscope showed wide intercellular spaces with fine bundles of keratin intermediate filaments around the nucleus. Fibrous connective tissue stroma with active fibroblasts and numerous mixed inflammatory cells (plasma cells, mast cells and numerous activated lymphocytes) were also revealed. Conclusions: Variable histopathological changes appeared in peri-implantitis soft tissue including hyperplastic nonkeratinized epithelium, mixed inflammatory cell infiltration with no statistical difference between T and B lymphocytes, excessive collagenization and vascular proliferation. Understanding these histopathological alterations may help clinicians in better management of peri- implantitis condition to ensure longevity of dental implants.}, keywords = {Histological,human,Immunohistochemical,peri-implantitis,Ultrastructural}, url = {https://edj.journals.ekb.eg/article_182116.html}, eprint = {https://edj.journals.ekb.eg/article_182116_b9256f1b60a14fe46f194d230304b4b4.pdf} } @article { author = {Ahmed, Naglaa}, title = {Temporomandibular joint asymmetry in asymptomatic skeletal class I patients}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2121-2132}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.68553.1557}, abstract = {Objectives Analysis of TMJ asymmetry in a sample of asymptomatic skeletal class I patients. Patients and methods This study was performed on one hundred patients requesting CBCT scans. All patients were skeletal class I pattern. They were all free of TMD symptoms, midline deviation of teeth, open bite, cross bite, congenital craniofacial abnormalities or any systemic diseases which may affect joint morphology such as rheumatoid arthritis. TMJ was evaluated through linear and volumetric analysis. Qualitative condyle position was also evaluated. All measures were compared on the right and left sides. Results There was relative asymmetry between right and left sides regarding all measured parameters. Condyle height showed the highest asymmetry, while condyle AP dimension was the least. There was absolute asymmetry between right and left sides regarding all measured parameters. fossa roof thickness showed the highest asymmetry, while condyle ML width was the least. On Correlating the R and L measurements, all correlations were positive with medium strength and were statistically highly significant. The highest correlation was shown in condyle volume while fossa roof thickness showed the least. The distributions of cases according to condyle position were almost statistically significantly different between right and left sides with 49 % percentage of agreement. Conclusion TMJ shows relative and absolute asymmetry in skeletal class I asymptomatic patients.}, keywords = {Temporomandibular joint,Cone beam computed tomography,asymmetry,Class I jaw relationship,Condyle}, url = {https://edj.journals.ekb.eg/article_182118.html}, eprint = {https://edj.journals.ekb.eg/article_182118_586e5d3534a19ab224ddcb8671245086.pdf} } @article { author = {gamal, hanan and Hammuda, Abdullah and Mohammed, Saher}, title = {Detection of Stem Cells in Dental Follicle and Benign Odontogenic Lesions by Immunohistochemical Expression of CD44 and c Myc}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2133-2142}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.70335.1570}, abstract = {The pathogenesis of odontogenic lesions could be explained by the presence of stem cells as those odontogenic lesions contain a small number of stem cells. Many odontogenic tumors have been reported to originate from stem cells that present in remnants of the dental lamina. The maintenance of stem cells in early dental development is regulated by variety of transcription factors such as OCT-4, SOX-2, CD44, Nanog, Stat-3 and c-Myc. The aim of this study is to evaluate and compare the immunoexpression of two stem cell markers, which are CD44 and c Myc, in dental follicles and in benign epithelial odontogenic lesions. The study included 8 specimens of dental follicle, 6 specimens of inflammatory cysts, 8 specimens of odontogenic keratocyst and 8 specimens of dentigerous cysts. All cases of dental follicles showed positive immunoreaction for the two markers. Regarding c Myc all cases showed +ve immunoreaction except (OKC) were –ve. Regarding CD44 all cases showed +ve immunoreaction. Results of the present study characterize the presence of stem cells in dental follicle and in benign odontogenic lesions illustrating their role in the pathogenesis of these odontogenic lesions.}, keywords = {Odontogenic lesions,Dental follicle,stem cells,CD44,c Myc}, url = {https://edj.journals.ekb.eg/article_182119.html}, eprint = {https://edj.journals.ekb.eg/article_182119_da7937a70d98837b66cbdbec287b0260.pdf} } @article { author = {Awwad, Rania and Gomaa, Dina}, title = {Detrimental Effects of Diabetes Mellitus on Dental Pulp Elements of Albino Rats.}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2143-2156}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.70164.1568}, abstract = {Diabetes Mellitus (DM) is a group of metabolic disorders with relative or absolute insufficiency of insulin secretion and/or concomitant resistance to the metabolic action of insulin on target tissues. Aim: to evaluate the effect of STZ-induced-DM on the dental pulp of albino rats histologically and ultra-structurally, and its effect on pulpal T&B lymphocytes immunohistochemically. Methods: Thirty male albino rats, were used in this study, divided into 2 groups: Group A (Control Group):10 rats received 2ml of citrate buffer (IP), was equally sub-divided into 2 sub-groups A1 & A2, according to the date of specimen collection. Group B (Experimental Group): 20 rats received an IP injection of STZ, equally sub-divided into 2 sub-groups; Sub-group B1 and B2 where the animals were sacrificed 2 and 4 weeks after establishment of DM respectively. permanent molars of each rat were split for pulp detachment, processed for histological, immuno-histochemical and ultra-structural examination. Results: H&E-stained sections of the pulp tissue of 2-weeks-diabetic rats showed degenerative changes. Ultra -structural examination showed degenerative changes affecting pulp elements. Immuno-histochemical examination of the pulp showed less T&B lymphocytes. Conclusion: DM led to degenerative changes of the pulp elements namely, cells, fibers, nerves, and vessels. DM also affected T&B lymphocytes.}, keywords = {Diabetes mellitus,streptozotocin,dental pulp}, url = {https://edj.journals.ekb.eg/article_182121.html}, eprint = {https://edj.journals.ekb.eg/article_182121_46323738acd9922fae8285f5697b0c95.pdf} } @article { author = {kabel, nihal and Zayed, Shaimaa and shamloula, hoda and mahmoud, ghada}, title = {Correlation between degree of pulp inflammation and success rate of MTA pulpotomy in primary teeth: Prospective Cohort Study.}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2157-2170}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.73417.1602}, abstract = {Objective: Many studies reported that the subjective clinical and radiographic examination lack the accuracy about the biological status of the pulp tissue. cytokines produced by dental pulp cells may reflect the degree of pulpal inflammation. The purpose of this study is to Correlate the cytokine levels of (IL-1α, IL-6, and IL-8) present in coronal dental pulp of primary molars with the success rate of MTA based vital pulpotomy over 18 months follow up period. Methods: 40 primary molars were enrolled. The cases are divided into group A (caries level code 5), group B (caries level code 6). Blood and pulp tissue were collected from the pulp cavity by sterile cotton pellet. The pellet was placed in 0.5ml (500 µl) phosphate buffered saline solution (PBS) to 1/10 final volume in a heparin-coated tube. Levels of cytokines IL-1 α, IL-6 and IL-8 were measured by using ELISA. Results: We found that IL-1 α, IL-6 and IL-8 were significant higher in group (B) than in (A). IL-6 showed the most significant value of expression in comparison to IL-1 α and IL-8 (p-value: 0.011, 0.014 & 0.027 respectively) which denote the prominent role of IL-6 during the inflammatory process in the pulp tissue of primary molars. Conclusion: IL-1 α, IL-6 and IL-8 cytokine expression in primary pulp molars increase with caries progression. Although the significant difference in the cytokine level expression between group A and B, a high success rate of MTA vital pulpotomy was found during the follow up period in both groups.}, keywords = {Cytokines,Vital pulp therapy,caries level,pulp inflammation. ELISA}, url = {https://edj.journals.ekb.eg/article_182123.html}, eprint = {https://edj.journals.ekb.eg/article_182123_3618dce2123bbf355aefab5c6aef7569.pdf} } @article { author = {Saleh, Nahed and al nawawy, marwa and darhous, mona and Shoeib, Mona}, title = {CLINICAL AND RADIOGRAPHIC EVALUATION OF ADVANCED PLATELET RICH FIBRIN IN THE PRESERVATION OF ALVEOLAR RIDGE FOLLOWING ATRAUMATIC TOOTH EXTRACTION: A RANDOMIZED CLINICAL TRIAL}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2171-2180}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.73934.1607}, abstract = {Aim: The study was aimed at determining the evaluation of hard and soft tissues at the extraction site when using advanced platelet rich fibrin compared to natural healing.Methodology: The present study was conducted on 30 subjects. The patients were allocated to receive either advanced platelet rich fibrin (APRF) (test group) or left for natural healing (control group). Alveolar ridge width was measured at 2 months and 4 months follow up and then it was subjected to a statistical analysis.Results: The buccolingual width result in the test group was decreased to 6.37±1.16mm while for the control groups, the values decreased to 5.95±0.53mm after 4 months with no statistical significance between the 2 groups. Both treatment protocols could not prevent bone resorption after 4 months. Conclusion: Despite the fact that ARP using APRF in our study did not totally avert bone loss, the values observed after 4 months were much lower than unassisted extraction.}, keywords = {Advanced Platelet rich fibrin,Socket preservation,hopeless tooth}, url = {https://edj.journals.ekb.eg/article_182126.html}, eprint = {https://edj.journals.ekb.eg/article_182126_9565e9ec846c62eb03f59b7e4b669aec.pdf} } @article { author = {Zayed, Shaimaa and hemed, reham}, title = {Comparing Effect of Annonaceae & Emetine Nanoparticles on NFĸB p65 Pathway in Tongue Squamous Cell Carcinoma (scc-25)}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2181-2191}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.74323.1610}, abstract = {Objective: The transformed cells in oral cancer secrete inflammatory mediators which generate an inflammatory microenvironment result in malignant transformation. Nuclear factor-κB (NF-κB) regulates a large number of genes which play a critical roles of the immune, inflammatory responses, cell proliferation, differentiation, and survival. Nanoparticles are 100 to 10,000 times smaller than human cells, could be designed to be highly selective for tumors and allow a slow release of active anticancer agents. We performed this study to investigate the effect of annonaceae nanoparticles and Emetine Emetine/Chitosan nanoparticles against scc-25 cell lines human tongue squamous cell carcinoma by studying their effect on NF-κB p65 expression. Methods: Cell Line cells were obtained from American Type Culture Collection, cells were cultured using DMEM (Invitrogen/Life Technologies). The effect of Annonaceae nanoparticles and EMETIN drug were investigated by Enzyme-Linked Immunosorbent Assay. The Signal is generated proportionally to the amount of bound NFĸB p65 and the intensity is measured by ROBONIK P2000 ELISA READER at 450 nm. Results: treatment of tongue scc-25 cells with both annonacea NP and Emetine/Chitosan NP revealed a significant increase in expression of NFĸB p65 in cancer cells by (227 & 261 pg/ml) respectively at incubation period 48 hours . While the control untreated cancer cells scc-25 revealed lower expression of NFĸB p65 at (147 pg/ml at incubation period 48 hour. Conclusion: Our findings demonstrate that annonaceae and Emetine nanoparticle might have regulatory effect on canonical NF-kb pathway by upregulation of NFĸB p65 expression signals.}, keywords = {Annonaceae,Emetine drug,Chitosan nanoparticles,NFkB65}, url = {https://edj.journals.ekb.eg/article_182127.html}, eprint = {https://edj.journals.ekb.eg/article_182127_c4b594d2d92ed5312a8c621ea3c6c91f.pdf} } @article { author = {Alabdulwahid, Ameera}, title = {Knowledge and Attitude of Female Undergraduate Dental Students and Interns Towards Radiation Safety and Protection}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2193-2198}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.74408.1613}, abstract = {Objective: The aim and objective of this study are to evaluate the level of knowledge and attitude about radiation hazards and safety practices among undergraduate female dental students and interns. Materials and Methods: An electronic questionnaire was distributed among female dental students and interns at the College of Dentistry, King Saud University, using the online service SurveyMonkey. Statistical Analysis: The collected data were analyzed using the IBM SPSS Statistical program version 26 (IBM Inc.NY, USA). Chi-square was used to test the association of knowledge and attitude with the academic level. Results: The surveys were completed by 156 dental students and interns, including 45 in the second year, 33 in the third year, 25 in the fourth year, 30 in the fifth year, and 23 dental interns. Conclusion: The results obtained from this study indicate that radiological safety and protection measures shout be emphasized more throughout the undergraduate level as well as in continuous teaching courses for dental interns.}, keywords = {Knowledge,Attitude,dental radiographs,Radiation Safety,Radiation protection}, url = {https://edj.journals.ekb.eg/article_182129.html}, eprint = {https://edj.journals.ekb.eg/article_182129_cc690fd4be72bf601b6227c323757ce3.pdf} } @article { author = {Kenawy, Sarah and Ellabban, Mohamed and Fadel, Ali}, title = {A Novel Frontal Sinus Index Protocol for Gender Determination: A Retrospective Lateral Cephalometric Study for Egyptians}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2199-2204}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.76583.1639}, abstract = {Abstract Aim: To develop and assess a new protocol proposed for sex determination using frontal sinus index measured from two dimensional lateral cephalometric radiographs Materials & Methods: The frontal sinus index was measured by two experienced radiologists and one experienced orthodontist on two hundred and one digital lateral cephalometric scans (Ninety-three males & One hundred and eight females). The maximum height and width of the sinus were measured, after that the height to width ratio was calculated. The mean height to width ratio of the sinus were tabulated and subjected to statistical analysis to determine the correlation to gender. Results: There was a high statistically significant difference between the frontal sinus index measured between males and females' groups. Conclusion: Frontal sinus index measured on lateral cephalometric radiographs by the developed novel protocol can be an indicator for gender identification in adult Egyptians and a larger sample multi-centric study is recommended.}, keywords = {Frontal Sinus Index,Lateral Cephalometric Radiograph,Gender,Forensic medicine}, url = {https://edj.journals.ekb.eg/article_182133.html}, eprint = {https://edj.journals.ekb.eg/article_182133_65a35bcfda206ee9b5d51787d6d88cce.pdf} } @article { author = {Elboraey, Mohamed}, title = {Effect of Change of Kilovoltage and Voxel Size on 3D Volume Measurement Using Cone Beam Computed Tomography}, journal = {Egyptian Dental Journal}, volume = {67}, number = {3}, pages = {2205-2212}, year = {2021}, publisher = {The Egyptian Dental Association (EDA)}, issn = {0070-9484}, eissn = {2090-2360}, doi = {10.21608/edj.2021.79717.1669}, abstract = {Purpose: The aim of this study is to study the impact of change in kilovoltage (KV) or voxel size on extracted 3D radiographic volume measurement of different sizes of endodontic files and to determine the optimal exposure parameters for volume measurement in CBCT. Methods: Eight different endodontic k files were radiographically exposed at three different kilovoltages using CBCT and divided into three groups, group 1 (80 KV ), group 2 (60 KV ), and group 3 (50 KV ), then exposed to two different voxel sizes, and divided into two groups, group a (300 μm) and group b (180 μm). The 3D radiographic volume and the actual files volumes were measured using ITK-SNAP software and water displacement method respectively. Finding: Regarding changes in the kilovoltages, no significant difference ( p ˃0.05 ) was reported between the mean of the 3D radiographic volume of groups 3 and 2 with the mean of actual volume with a mean difference of 1.1 ±7.7 mm3 and 2.6 ±9.6 mm3 respectively. The results for voxel size change, showed no significant difference between the mean of the 3D radiographic volume of group (b) and actual volume group with a mean difference of 1.9 ±7.8 mm3. In the other hand, the difference between the mean of volume measurement at group (a) and actual group became significant with a mean difference of 10.5 ±5.7 mm3 was found. Conclusion: it concluded that both changes in kilovoltage and voxel size parameters affect significantly the 3D volume measurement and reconstruction.}, keywords = {voxel size,3D Volume,CBCT,ITK-SNAP}, url = {https://edj.journals.ekb.eg/article_182134.html}, eprint = {https://edj.journals.ekb.eg/article_182134_b3e36a8ce48cd8c8ec60c92b19f4509e.pdf} }