ORIGINAL_ARTICLE
THE POSSIBLE EFFECT OF OMEGA-3 FATTY ACIDS VERSUS INSULIN ON EXPRESSION OF ACTIVATED CASPASE-3 AND RUSSELL BODIES DEVELOPMENT IN BUCCAL
Background: Damage of tissue repair and dysfunction of the oral mucosa are usually associated with the diabetic condition. Apoptosis plays a functional role in limiting diabetic repair. Russell bodies, an uncommon finding that may be associated with chronic inflammatory conditions. Although insulin is the mainstay of diabetes treatment, it has prominent side effects. Episodes of severe hypoglycemia and increased mortality rate are associated with insulin therapy. Cod liver oil (CLO) is an important source of long-chain omega-3 (ω-3) fatty acids that have antioxidant and anti-inflammatory properties.Objective: The present study was designed to determine the adaptive apoptotic alterations accompanying diabetes in the buccal mucosa with the possibility of occurrence of the so-called Russell bodies and the possible role of long-chain ω-3 fatty acids versus insulin supplementation in enhancement of the buccal mucosa in streptozotocin (STZ)-induced diabetic rats.Design: Sixty adult male Swiss albino rats (200-250 gm) were selected for this study. The animals were randomly divided into four groups (fifteen rats each): Group I (Control group), Group II (Diabetic untreated group), Group III (Insulin treated group) and Group IV (Cod liver oil treated group). At the end of the experimental period (four weeks), the rats were sacrificed and the specimens were obtained from the mucosa of the cheek of both sides. The sections were examined histologically, immunohistochemically and histomorphometrically. Statistical analysis: Data obtained from histomorphometric analysis were statistically described in terms of mean ± standard deviation (± SD). Results: Histopathologic examination of Group I revealed the normal histological features of the buccal mucosa. In Group II several histological changes in the epithelial layer of the buccal mucosa were noticed. These changes include; atrophy in the epithelium, evidences of cells undergo degeneration, nuclear changes, ill-defined cell membrane, cytoplasmic vacuolations, thickening of stratum granulosum (hypergranulosis) which was accompanied by hyperkeratosis. Moreover, the lamina propria showed differential orientation and arrangement of the collagen fiber bundles, areas of degeneration and hyalinization in addition to fibroblasts that showed signs of degeneration. Furthermore, the lamina propria was strongly infiltrated by inflammatory cells and invaded by multiple Russell bodies scattered throughout the connective tissue (C.T) and dilated blood vessels (BVs); lined by swollen endothelial cells and engorged with red blood cells (RBCs) were observed. Some of these changes were still observed in Group III that received insulin treatment. Treatment with CLO in Group IV resulted in histological features resembling nearly those of the control group (Group I). The least immuno-expressions for caspase-3 and CD-138 were detected in Group I, followed by Group IV, then Group III and subsequently Group II. The histomorphometric analysis supported the previous results as Group I showed the highest mean epithelium thickness, followed by Group IV, then Group III and the least value was for Group II. On the other hand, Group I showed the least mean keratin thickness and the least mean area percentage of both caspase-3 and CD-138 immunoreactivities, followed by Group IV, then Group III and the highest values were for Group II. There was statistically highly significant difference between the studied groups.Conclusions: Diabetes has deleterious effect on the structure of the buccal mucosa. The raised levels of capase-3 in diabetic buccal mucosa are related to increased Russell bodies (Mott cells) formation. Insulin can’t completely inhibit the complications of diabetes. However, ω-3 fatty acids present in CLO can inhibit to a great extent the abnormalities caused by diabetes.
https://edj.journals.ekb.eg/article_74772_772d5f10475426abf4166bdaa1667e68.pdf
2017-01-01
415
438
10.21608/edj.2017.74772
diabetes
Buccal mucosa
apoptosis
Russell bodies
Mott cells
Insulin
cod liver oil
omega-3 fatty acids
Amany
Rabea
amanyrabea1@gmail.com
1
Lecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Future University.
AUTHOR
Marwa
Abdel-Hameed
2
Lecturer, Oral Biology Department, Faculty of Dentistry, Ain Shams University
AUTHOR
ORIGINAL_ARTICLE
COMPARATIVE STUDY ON THE POSSIBLE EFFECT OF COD LIVER OIL VERSUS INSULIN ON PAROTID SALIVARY GLANDS OF STREPTOZOTOCIN-INDUCED DIABETIC ALBINO RATS
Background: The diabetic condition is frequently associated with impaired functions of salivary glands, testified by both morphological deterioration of the gland and by altered salivary composition. Abnormal apoptosis has been implicated in salivary glands of diabetic rat models. Amyloidosis constitutes a group of diseases in which proteins deposit in tissues as insoluble fibrils, causing progressive organ dysfunction. Although insulin and oral hypoglycemic agents are the mainstays of diabetes treatment, they have prominent side effects and fail to alter the course of diabetic complications. Cod liver oil (CLO) is an important source of long-chain omega-3 (ω-3) fatty acids as well as vitamins A, E and D. CLO has antioxidant effect especially on parotid salivary glands.Objective: The present study was undertaken to investigate the possible role of CLO versus insulin supplementation in enhancement of parotid salivary glands in streptozotocin (STZ)-induced diabetic rats.Design: Sixty adult male Swiss albino rats (200-250 gm) were selected for this study. The animals were randomly divided into four groups (fifteen rats each): Group I (Control group), Group II (Diabetic untreated group), Group III (Insulin treated group) and Group IV (Cod liver oil treated group). At the end of the experimental period (four weeks), the rats were sacrificed and the parotid salivary glands were dissected out. The sections were examined histologically, immunohistochemically, histomorphometrically and by fluorescence staining technique. Statistical analysis: Data obtained from histomorphometric analysis were statistically described in terms of mean ± standard deviation (± SD). Results: Histopathologic examination of Group I showed the normal histological features of parotid gland. Group II revealed apparent reduction in acinar size, ill-defined acinar and ductal cells outlines, nuclear changes, acinar and ductal cells degeneration, lipid droplets, dilatation of the duct system lumina and stagnated salivary secretion in the lumina of striated and excretory ducts. Moreover apparent decrease, hyalinization and degeneration in the fibrous connective tissue (C.T) fibroblasts showing signs of degeneration and dilated blood vessels (BVs) with swollen endothelial cells were noticed. Group III showed better histological features than those of Group II, while Group IV showed histological features resembling nearly those of the control group (Group I). The least immuno-expression for caspase-3 and the minimum fluorescence with thioflavin-T stain were demonstrated in Group I, followed by Group IV, then Group III and subsequently Group II. The histomorphometric analysis supported the previous results as Group I showed the highest mean acinar area fraction, followed by Group IV, then Group III and the least value was for Group II. On the other hand, Group I showed the least mean area percentage of both caspase-3 immunoreactivity and thioflavin-T fluorescence, followed by Group IV, then Group III and the highest values were for Group II. There was statistically highly significant difference between the studied groups.Conclusions: Diabetes has deleterious effect on the structure and function of parotid salivary glands. Moreover, it has a major role in tissue damage through development of amyloidosis. Insulin can’t completely inhibit the complications of diabetes. However, CLO has great potential to inhibit the abnormalities caused by diabetes.
https://edj.journals.ekb.eg/article_74778_3c84f6001decd78e2ae35fb041ca18b8.pdf
2017-01-01
439
467
10.21608/edj.2017.74778
diabetes
Parotid salivary gland
apoptosis
amyloidosis
Insulin
cod liver oil
Amany
Rabea
amanyrabea1@gmail.com
1
Lecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Future University.
AUTHOR
ORIGINAL_ARTICLE
THE ACCURACY OF CONE BEAM COMPUTED TOMOGRAPHY IN ASSESSMENT OF TOOTH AND ROOT LENGTH MEASUREMENTS: IN-VITRO STUDY
Aim: Assessment of the accuracy of cone beam computed tomography in tooth and root length measurements. Methods: 50 human extracted teeth were used in the current study. The 50 premolars were divided into two groups: Group (1) consisted from 25 double rooted maxillary first premolars while Group (2) comprised 25 single rooted mandibular first premolars. Teeth and roots lengths were measured for each group with a manual caliper and from CBCT images. Physical and CBCT measurements were taken twice by two observers and the average values were used to avoid interobserver errors to compare between the manual caliper and the CBCT measurements and then the accuracy of CBCT was assessed. Results: Intraclass correlation between CBCT vs. Physical Measurements ranged from (-0.04 to -0.759) which indicates a complete disagreement between the physical measurements and CBCT measurements for all tested groups. Conclusion: Dental measurements taken on digital models are not as accurate as those taken manually. There is always underestimation of CBCT measurements compared to physical measurements.
https://edj.journals.ekb.eg/article_74782_efac0f90199506e3fcabd6923a558da9.pdf
2017-01-01
469
476
10.21608/edj.2017.74782
Omniya
Abu El-Dahab
1
Lecturer of Oral and Maxillofacial Radiology, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
AUTHOR
Iman
Dakhli
2
Lecturer of Oral and Maxillofacial Radiology, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
HISTOLOGICAL STUDY OF THE EFFECT OF PROPOLIS ON GINGIVAL TISSUE OF ALBINO RAT
Aim The aim of the present work was to study the proliferative and maturative influence of propolis on the gingival tissues in rats using routine H&E stain and immunohistochemical detection of any possible changes in proliferating cell nuclear antigen (PCNA) and E- cadherin in the surface epithelium of the gingiva so that ,we get an answer for the question; Is it beneficial if the dentist use propolis as a constituent of surgical dressing as well as mouth wash?Materials and methods Thirty adult male albino rats with 120-150 gram body weight were used in this investigation. The animals were divided into 2 groups 15 animals each; Group I animals served as controls, and supplied with a daily oral dose of 1ml distilled water. Group II animals were treated with propolis in a daily oral dose of 50 mg/kg body weight using curved metallic oro-pharyngeal tube. Propolis tablets were dissolved in distilled water to a concentration of 5mg/1ml. The animals were fed natural diet and given drinking water adlibitum throughout the whole experimental period, which lasted for 3 months. At the end of the experiment, the animals of both groups were sacrificed by cervical dislocation. gingival Specimens of both sides were taken from the region of the first molars, The specimens were fixed in 10% neutral buffered formalin, processed to be embedded in paraffin. Six microns thick sections were cut and stained Hematoxylin and eosin stain for histological detection any structural changes in the epithelium, lamina propria. Immunohistochemical staining procedure using a- The universal dakocytomationlabeled streptavidin- biotin system horseradish peroxidase enzyme for detection of any possible changes in (PCNA) of the surface epithelium. b- The ultravesion mouse tissue detection system using antimouse monoclonal antibody for demonstration of E- cadherin to detect any possible changes.Results The present work revealed that supplementation of propolis to the rats may activate the proliferation, regeneration and maturation of the epithelial cells the gingival tissue and improve vascularity and organization of collagen fibers of gingival subepithelial lamina propria Conclusion Propolis solution enhances the integrity and barrier function of the gingival epithelial cells and collagen fiber, thus it increases the viability and physiological health of gingival cells.Recommendation Propolis is recommended to be used as a beneficial constituent of mouth wash and surgical dressing after fulfilling all the required investigations on human.
https://edj.journals.ekb.eg/article_74785_17662027f22513e0d24e94623fd1322b.pdf
2017-01-01
477
490
10.21608/edj.2017.74785
Propolis
gingiva dressing
Wound healing
proliferation
Gingiva
Mervat
Yossef
dr.mervathawas@gmail.com
1
Lecturer in Oral Biology Department, Faculty of Dentistry, Suez Canal University.
AUTHOR
Dalia
Bayoumi
2
Lecturer in Dental Material Department, Faculty of Dentistry, Suez Canal University.
AUTHOR
ORIGINAL_ARTICLE
COMBINED EFFECT OF FLUORIDE GEL AND DIODE LASER 980 NM ON ROOT CARIES INHIBITION
Aim: To study the evaluation of the combined effect of Diode laser and fluoride gel on root dentin demineralization and compare between the use of laser only & laser with fluoride in root caries. Methods: Tooth preparation & grouping, fluoride treatment, laser treatment, fluoride & laser treatment, tooth-sectioning and artificial caries application & environmental scanning electron microscope analysis. Results: The effect of artificial caries on the fluoride and diode laser specimens showed an augmentation of the caries prevention mechanism. Conclusion: Our results strongly dictate a synergistic rather than an additive effect of the combined diode laser and fluoride gel treatments in root caries inhibition.
https://edj.journals.ekb.eg/article_74789_52dc60cf964c0df49144672bc7226c2d.pdf
2017-01-01
491
498
10.21608/edj.2017.74789
Mohamed
Mohamed
m.abdelrahman@tudent.edu.sa
1
Assistant Professor of Oral Pathology - Faculty of Dentistry, Al-Azhar University
AUTHOR
Haytham
Moharrum
2
Lecturer In Dentistry-National Institute of Laser Enhanced Science, Cairo University
AUTHOR
ORIGINAL_ARTICLE
HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY ON THE EFFECT OF LONG TERM TREATMENT WITH AMADOL (TRAMADOL HYDROCHLORIDE) ON THE LINGUAL
Objectives: This study aimed to evaluate the effect of Amadol (Tramadol hydrochloride) on the lingual mucosa of male albino rats through histological examination of their lingual mucosa to detect any structural changes, scanning electron microscopic examination of their lingual mucosa to detect any ultrastructural changes and immunohistochemical localization of proliferating cell nuclear antigen (PCNA).Materials and methods: Thirty adult male albino rats divided into 2 group. Group I (10 animals) served as control. Group II (20 animals) received Amadol (tramadol hydrochloride) in daily oral dose of 60 mg/kg body weight using metallic curved oropharengeal tube for 4months. Results: The light microscopic examination of the tongue of the rats treated with Amadol drug showed degenerative changes that involved the surface epithelium and lamina propria of their lingual mucosa. The immunohistochemical results of the tongue of Amadol group showed weakly positive PCNA staining reactivity of the nuclei of the basal and parabasal cells of the surface epithelium. Scanning electron microscope results indicate the histological and imunohistochemical results, as showed marked degenerative changes of dorsal surfaces of the tongue.Conclusion: Tramadol prescription, after weighing its risks and benefits, should be used with limits and under supervision.
https://edj.journals.ekb.eg/article_74793_24259fabffc86c7f2295742e61e0af1b.pdf
2017-01-01
499
508
10.21608/edj.2017.74793
tramadol
lingual mucosa
PCNA
Rasha
Taha
1
Lecturer of oral Biology Department, Faculty of Dentistry , Suez Canal University.
AUTHOR
ORIGINAL_ARTICLE
EVALUATION OF THE BONE REGENERATION EFFICACY OF MAGNESIUM BIOACTIVE GLASS VERSUS ZINC BIOACTIVE GLASS BOTH LOADED WITH BONE MORPHOGENETIC PROTEIN-2 IN EXTRACTION SOCKETS OF DIABETIC ALBINO RATS
Background: Delayed wound healing is one of the major complications of diabetes. The aim of the present study was to enhance bone regeneration in extraction sockets of diabetic rats through controlled release of bone morphogenetic protein-2 (BMP-2) from 2 types of bioactive glass scaffoldsMethods: Ninety male Albino rats were utilized in this study. Diabetes was induced by 4 daily intraperitoneal injections of streptozotocin. The first right mandibular molar was extracted for all rats. The rats were randomly assigned into three groups (GI, GII and GIII) (30 rats each) according to the date of sacrifice 3,7 and 9 days after extraction. The three main groups were subdivided into: Control subgroup (Con), Mg (Mg) and Zn (Zn) Bioactive glass subgroups. The bone regenerative efficacy of the scaffold materials were evaluated histologically, immunohistochemically and histomorphometrically. Results: A significant increase in the newly formed bone area percentage has been recorded in the Zn subgroup more than the Mg subgroup within GII and GIII where (P<0.001). The immunohistochemical results revealed a significant increase in vascular endothelial growth factor (VEGF) expression within GI and GIII subgroups where (P=0.002 and P=0.001) respectively. On the other hand there was a non significant increase in VEGF expression between GII different subgroups (P=0.267).Conclusions: Mg and Zn enriched bioactive glass mixed with BMP-2 were capable to enhance wound healing and bone formation in extraction sockets of diabetic rats. Zn bioactive glass was more effective as it enhanced angiogenesis in the healing sockets.
https://edj.journals.ekb.eg/article_74796_359d4888209a52a93b543837d5160e05.pdf
2017-01-01
509
522
10.21608/edj.2017.74796
Mg bioactive glasses
Zn bioactive glasses
Scaffolds
Extraction sockets
Streptozotocin induced diabetic rats
Marwa
Abbass
1
Assistant Professor, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University.
AUTHOR
Zeinab
Salem
2
Lecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University
AUTHOR
ORIGINAL_ARTICLE
CLINICAL AND HISTOLOGICAL EVALUATION OF GINGIVAL HEALING AFTER GINGIVECTOMY AND LOW-LEVEL LASER THERAPY
Background: Low-level laser therapy (LLLT) may induce morphological and cellular processes, which are involved in wound healing. Thus, increasing interest in the matter of LLLT is based on its ability to provide minimally invasive and painless treatments for the patient as well as increasing his comfort following surgery. Objective: The purpose of this study was to assess the clinical and histological effect of LLLT on healing of gingiva after gingivectomy. Methods: Twenty patients with inflammatory gingival enlargement on their maxillary or mandibular anterior region were included in this study. Patients were divided randomly into two groups (Test group=ten patients) after gingivectomy a diode laser (660 nm) was applied to the wound area immediately post-surgery (day 1) and at days 3, 5 post-surgery. The control group (ten patients) did not receive laser irradiation. Healing was evaluated clinically using Healing score and Visual analogue scale VAS. For histological evaluation, mucosal defect measuring 2 mm in diameter and 0.2 mm in wall thickness was created into the hard palate of eighteen male Dawley rats. For the test group (9 rats) LLLT was applied with the same settings and intervals used in the clinical part of the study, while for the control group (9 rats) no laser irradiation was applied. Results: The laser-treated group had significant improvement regarding the healing score at 5, 7 and 14 days p.s., and significant improvement in VAS score was observed at day 3 and 5 p.s., than the control group. The histological sections revealed a thin layer of epithelium covering the wound after 4 days in the test group in contrast to the control group. Also, complete epithelization of the wound area with keratin formation and revascularization after 7 days was observed in the test group. Conclusion: LLLT can be used as an effective adjunctive treatment following gingivectomy procedure to promote healing of oral mucosa.
https://edj.journals.ekb.eg/article_75002_618231a0e3bf6ca37b7eb085113dc782.pdf
2017-01-01
523
532
10.21608/edj.2017.75002
Gingivectomy
Low level Laser
Healing
Clinical
Histological evaluation
Azza
Koura
1
Assistant Professor of Oral Biology Department, Faculty of Dentistry, Alexandria University Egypt.
AUTHOR
Marwa
Madi
2
Lecturer of Oral Diagnosis and Radiology Department, Faculty of Dentistry, Alexandria University Egypt.
AUTHOR
Nancy
Mamdouh
3
Lecturer of Oral Biology Department, Faculty of Dentistry, Alexandria University Egypt.
AUTHOR
ORIGINAL_ARTICLE
CLINICAL APPLICATION OF RADIESSE AND HYALURONIC ACID GEL FOR TREATMENT OF PAPILLAE DEFICIENCIES IN THE ESTHETIC ZONE
Background: A black triangle or an open gingival embrasure is one of the main problems that compromise esthetics. Different surgical techniques have been utilized to resolve this problem. Consequently, minimally invasive techniques using filler injections have been recommended. Hyaluronic acid (HA), a naturally occurring polysaccharide; was widely used in treatment of deficient interdental papillae. On the other hand, Radiesse is calcium hydroxide dermal filler used in facial rejuvenation. This study was performed to compare the effect of Radiesse and hyaluronic acid gel in treatment of deficient interdental papillae. Materials and Methods: A total of 30 female patients with 40 deficient interdental papillae were assigned into two groups A and B. Group A received hyaluronic acid gel injection while group B received Radiesse gel injection. Clinical data included papillary marginal gingival index, probing depth, clinical attachment loss, papilla presence index and height of interdental papilla. Data were taken at baseline, after three weeks, three and six months. Results: Significant improvement occurred in both groups but this improvement was more prevalent in Radiesse group compared with HA group. There was a decline in the papillary height in the HA group after three and six months while in Radiesse group more long-lasting effect was denoted. Conclusion: It was concluded that both fillers were biocompatible and safe for treatment of interdental papilla deficiency. Though, Radiesse gel was more effective and long-lasting compared with hyaluronic acid.
https://edj.journals.ekb.eg/article_75003_18292bed6a4c85c912aae862c4af02d0.pdf
2017-01-01
533
545
10.21608/edj.2017.75003
Hyaluronic acid
image J
interdental papilla
papilla presence index
Radiesse
Heba
Shawky
1
Lecturer of Oral Medicine and Periodontology, Oral Medicine and Periodontics Department, Faculty of Dentistry, Pharos University in Alexandria, Egypt.
AUTHOR
Mahinour
Darwish
2
Assistant Lecturer of Oral Medicine and Periodontology, Oral Medicine and Periodontics Department, Faculty of Dentistry, Pharos University in Alexandria, Egypt.
AUTHOR
ORIGINAL_ARTICLE
MICROBIAL BIODIVERSITY AND ANTIBIOTICS IN PERIODONTAL DISEASES A LITERATURE REVIEW
The Oral cavity is a reservoir of bacteria with high biodiversity, however, not all species can cause invasive diseases. Identification of pathogenic bacteria in the oral lesions is challenging because cultivation of specimens from this non sterile site invariably yields a diversity of bacteria, making associations between cultivated bacteria and particular oral lesions difficult. Periodontitis is a multifactorial disease which might have a systemic or hereditary component in addition to its bacterial aetiology. It is highly prevalent worldwide, affects 30-50% of the population in developed countries, but only ~10% present with severe forms. The microbial aetiology of periodontal disease has been proved since long time ago, approximately 500 species or more have been detected in the gingival sulcus, some of them are widely regarded as major pathogens in periodontitis. Periodontal microbiota are more heterogeneous than earlier believed, in dentistry, gram-negative organisms were considered to be the predominant bacteria in periodontitis; however, gram-positive organisms found in deep, diseased sites are proposed to be the most important pathogens in periodontitis recently. Using antibiotics in controlling periodontal diseases is essential, which either suppress the growth of microorganism or destroy them. Drugs used to treat periodontal disease can be used systemically or locally, however, some periodontopathic bacteria can develop resistance to certain antimicrobials specially in refractory periodontitis and aggressive periodontitis cases.
https://edj.journals.ekb.eg/article_75004_843888f732d70d9eddac0b8a6e1cc132.pdf
2017-01-01
546
554
10.21608/edj.2017.75004
Periodontal pathogens
Periodontal disease
Microbial biodiversity
Antibiotic resistance
Hossam
Eid
1
PhD, Oral Medicine and Periodontology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
AUTHOR
Aly
Shoun
2
MSc, Assistant lecturer Microbiology & Immunology Department, Faculty of Pharmacy, Badr University, Egypt.
AUTHOR
Rana
Alkady
3
Master in Pharmacology, Alexandria University hospital, Ministry of Health, Egypt.
AUTHOR
ORIGINAL_ARTICLE
ESTIMATION OF PENTRAXIN-3 AND TUMOR NECROSIS ALPHA LEVELS IN GINGIVAL CREVICULAR FLUID OF CHRONIC PERIODONTITIS PATIENTS
Chronic Periodontitis (CP) is a disease characterized by interactions between microbial pathogens and host immune response that have a crucial role in its initiation and progression via the release of inflammatory mediators such as tumor necrosis factor- α (TNF-α). Pentraxin-3 (PTX3), the first long pentraxin described, is produced in response to pro-inflammatory cytokines by a variety of cells including those abundant in periodontal tissues. The aim of the present study was to investigate the role of PTX3 compared to TNF -α as a diagnostic and prognostic marker for CP. Gingival Crevicular Fluid (GCF) samples were taken from 53 CP patients before and after scaling and root planing (SRP) as well as from 50 periodontally healthy subjects as a control group. The GCF samples were tested for PTX3 and TNF-α levels by Enzyme-Linked Immunosorbent Assay (ELISA). Differences between CP patients and healthy subjects regarding clinical parameters and tested biomarkers in GCF were assessed. Moreover, the correlations between them were calculated. Out of 465 female patients examined, 53 patients were diagnosed as CP with a prevalence rate of 11.4%. The mean values of periodontal parameters were significantly reduced after treatment. Mean TNF-α and PTX3 levels were higher in CP patients (935.27 ± 264.21) (2.49 ± 0.537) than in the healthy group (772.32 ±0.148) (1.077 ± 0.084) respectively, with a significant difference between both groups (p < 0.05). Furthermore, these levels were reduced after treatment with a highly statistically significant difference. Positive correlations between the mean values of TNF-α, PTX3 levels, and clinical parameters were found whereas, PTX3 was more positively correlated. In conclusion, both PTX3 and TNF-α in GCF, previously recommended to be used as diagnostic markers for CP, can be also used as prognostic markers for follow-up of CP patients in addition to clinical and radiological parameters. However, further large-scale studies on both genders are recommended to confirm their role as prognostic markers.
https://edj.journals.ekb.eg/article_75005_7a10e19d4391ac3165f201102f2943b0.pdf
2017-01-01
555
563
10.21608/edj.2017.75005
Ebtehal
Hamdy
1
Department of Oral Medicine, Periodontology and Diagnosis, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
AUTHOR
Ibtesam
Afifi
2
Department of Medical Microbiology & Immunology, Faculty of Medicine, Tanta University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
STUDY THE INFLUENCE OF ANTIOXIDANT THERAPY ON AGE- RELATED CHANGES IN THE BUCCAL MUCOSA OF RATS (HISTOLOGICAL AND IMMUNOHISTOCHEMICAL INVESTIGATION)
Aim The aim of the present work was to study the histological and immunohistochemical impact of antioxidant supplementation on age- related changes in the buccal mucosa of rats using routine H&E stain and immunohistochemical detection of any possible changes in NF-κB (nuclear factor kappa) in the surface epithelium, lamina propria as well as buccal salivary glands. Materials and methods Fifty young adults albino male rats with average body weight 100± 10 grams and 2 months of age were used in this investigation. The animals were divided into 5 equal groups 10 aminals each. They were caged, 5 animals per cage and fed diet consisting of coarse corn barley and powdered milk. Diet and drinking water were supplied adlibitum throughout the whole experimental period which lasted for 9 months. Group I: animals served as control. 5 animals were sacrificed by cervical dislocation after one month to serve as young controls, while the rest remained till the end of the experiment to serve as old controls. Group II: animals received vitamin A. Group III: animals received vitamin C. Group IV: animals received Selenium. Group V: animals received combined vitamin A, C and Selenium in the same doses given to group II, III, IV respectively. Preparation of the diet mixed with antioxidants were repeated several times until the experiment was over. At the end of the experiment, The animals of different groups were scarified by cervical dislocation. Their buccal mucosa were dissected out, fixed in 10% neutral buffered formalin, washed, dehydrated in ascending grades of ethyl alcohol, cleared in zylene and embedded in paraffin. Six micron thick sections were cut and stained with Hematoxylin and eosin for histological examination. Masson’s trichrome stains for collagen evaluation. Immunohistochemical localization of NF-κB (nuclear factor kappa) for detection of any possible changes. Results The histological and immunohistochemical results revealed aging caused atrophic and degenerative changes in the oral mucosa associated with increased expression of nuclear factor kappa. Consuming antioxidant vitamins as vitamin A, vitamin C or selenium separately partially modulates the action of aging process and increase the regenerative capacity of the oral mucosa and oral salivary glands. Consuming combined antioxidant vitamin A, vitamin C and selenium had a powerful and beneficial synergizing antiaging effect on the aforementioned tissues.Conclusion The balance between oxidation and antioxidation (redox balance) is critical in maintaining a healthy biological system. Our histological and immunohistochemical investigation proved the important role of antioxidant for keeping the integrity and health of the mucous membrane and salivary glands of the buccal mucosa of aging rats under investigation.Recommendation Improvement of dietary intake by consuming neutral foods rich in antioxidant vitamin A, C and selenium is of utmost importance to gain the antiaging benefits and to prevent age –related degenerative diseases.
https://edj.journals.ekb.eg/article_75006_8950f6388b92d835737d2461d7c4bf7b.pdf
2017-01-01
565
578
10.21608/edj.2017.75006
aging
vitamin A
vitamin c
selenium
antioxidant
NF-κB
Buccal mucosa
Mervat
Youssef
1
Lecturer of Oral Biology, Oral Biology Department, Faculty of Dental Medicine, Suez Canal University.
AUTHOR
ORIGINAL_ARTICLE
SALIVARY INSULIN LIKE GROWTH FACTOR BINDING PROTEIN-3 AND TRANSFERRIN LEVELS IN PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA VERSUS ORAL LICHEN PLANUS
Objective: To gather preliminary data concerning local salivary levels of insulin like growth factor binding protein-3 (IGFBP-3) and its binding protein transferrin (Tf) in patients suffering from oral squamous cell carcinoma (OSCC) and oral lichen planus (OLP) compared to normal controls and to correlate it with clinical picture in OLp and histologic grading in OSCC. Materials and methods: Salivary samples from 40 patients suffering from OSCC (20 poorly and 20 moderately differentiated), 40 patients suffering from OLP (clinically evaluated using REU scoring system) and 40 controls were collected and TF and IGFbP-3 levels were estimated using enzyme linked immunosorbent assay (ELISA), for statistical analysis, ANOVA followed by Tukey’s post hoc and Pearson correlation tests were used. Results: Significantly higher salivary levels of IGFBP-3 and Tf were recorded in different histological grades of SCC in comparison to Olp. Control group recorded the lowest significant values compared to OLP and OSCC (p<0.0001). A week positive correlation was recorded between levels of Tf and IGFBP-3 in all groups. As well, a week positive correlation between IGFBP-3 and Tf levels with REU in OLP and histological grades in OSCC was shown, except for the correlation between TF and OSCC histologic grading that was strong positive. Conclusions: IGFBP-3 and Tf seems to play a role in pathogenesis of both OSCC and Olp, and could be considered as a reliable marker in diagnosis of OSCC and OLP, However, further studies are required .
https://edj.journals.ekb.eg/article_75007_0845e657a9d8bdb1b843f95eb77e67eb.pdf
2017-01-01
579
589
10.21608/edj.2017.75007
Naglaa
Elwakeel
1
Oral Medicine, Diagnosis, Periodontology and Radiology, Faculty of Dental Medicine, Al Azhar University (Girls Branch), Cairo, Egypt; Oral Medicine, Diagnosis, Periodontology, Faculty of Dentistry, MSA University, Giza, Egypt.
AUTHOR
Olfat
Shaker
2
Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
POSSIBLE PROTECTIVE EFFECT OF GINGER ON THE BUCCAL MUCOSA OF METHOTREXATE TREATED MALE ALBINO RATS (MOLECULAR AND HISTOLOGICAL EVALUATION)
Introduction: Methotrexate (MTX) is widely used as a chemotherapeutic agent. However, severe side effects and toxic conditions limit its efficacy. Recently, it has been focused on the protective effect of plant derived natural compounds, such as ginger, against chemicals inducing toxicity. Aim: The aim of the present study was to elucidate the possible protective effect of ginger administration on the buccal mucosa of rats under MTX treatment. Material & methods: Eighteen adult male albino rats were divided into three groups (six animals each). Group I (Control group): rats were sacrificed at the end of the experimental design. Group II (MTX group): received 15mg/Kg/day of MTX for 3 successive days. Group III (MTX and ginger group): received ginger (200mg/kg/day) for 15 successive days then, MTX as in group II concomitantly with ginger administration daily. Rats of groups II and III were sacrificed immediately after the last dose of MTX. Specimens were prepared for histological examination and molecular study.Results: Histopathological examination of group II revealed atrophy and complete loss of normal architecture with cellular deformities within the buccal mucosal cells. These changes were markedly alleviated in group III specimens. Molecular results supported the histological results which presented marked decrease in DNA relative concentration in group II when compared to the control one, while less obvious decrease in DNA relative concentration was detected in group III. Conclusion: Pre and co administration of ginger protected and ameliorated most of the damage caused by MTX in the buccal mucosa structure and DNA.
https://edj.journals.ekb.eg/article_75008_5f5a83292d7cb1224be6ac750ccdc05c.pdf
2017-01-01
591
604
10.21608/edj.2017.75008
Buccal mucosa
Methotrexate
Ginger
DNA
Mohamed
Shredah
1
Ass. Professor, Oral Biology Department, Faculty of Dentistry, Damanhour University
AUTHOR
Mona
El Deeb
2
Ass. Professor, Oral Biology Department, Faculty of Oral and Dental Medicine, Future University.
AUTHOR
ORIGINAL_ARTICLE
THE EFFECT OF ORALLY INGESTED ATORVASTATIN ON THE MASSETER MUSCLE OF WHITE ALBINO RATS (HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDIES)
Statins are important class of cholesterol lowering drugs that provide cardio-protective effect. They act by inhibiting 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG Co reductase), the rate limiting enzyme involved in cholesterol biosynthesis pathway. A lot of researches are in progress to reveal the other effects of statins including anti-inflammatory activity and antioxidant activity properties. However, statin-induced myopathy, which is considered a side effect of statins’ use, limits their utilize. Statins induced myopathy may result from reduced muscular coenzyme Q10 levels. This study was carried to evaluate the effects of ingestion of two different doses of atorvastatin for six weeks on the masseter muscle of rats. Eighteen males Sprague Dawley albino rats weighted 200-250 grams at the age of 6-8 weeks were used in this study. They were divided into 3 groups, group I (control group), group II (20 mg/kg b.w atorvastatin received rats) and group III (40 mg/kg atorvastatin received rats). Masseter muscle specimens were fixed in formal saline solution, dehydrated using ascending grades of alcohol, cleared using xylene then infiltrated with paraffin and finally embedded in it. Histological results revealed fragmented and disorganized muscle fibers, centrally located nuclei and clear external rims around some fibers. Immunohistochemical results using Bax showed increased in the cytoplasmic immunopositivity in the muscle fibers of both experimental groups. These results suggested that atorvastatin exhibits significant myotoxicity on rats’ masseter muscle. Atorvastatin side effects were found to be dose dependent.
https://edj.journals.ekb.eg/article_75009_0a08734fd5816c8d7f0319b00c8cfd7c.pdf
2017-01-01
605
614
10.21608/edj.2017.75009
Atorvastatin
masseter muscle
Bax
myotoxicity
Mohammed
Shredah
1
Assistant Professor, Oral Biology Department, Faculty of Dentistry, Damanhour University.
AUTHOR
Saher
Sayed
2
Lecturer of Oral Biology, Oral Biology Department, Faculty of Dentistry, Minia University.
AUTHOR
ORIGINAL_ARTICLE
THE EFFECT OF ENERGY DRINKS ON TEETH HYPERSENSITIVITY
Background: Considering the current high consumption of energy drinks among university students, the aim of the present study was to evaluate the correlation between increased energy drinks consumption and teeth hypersensitivity by studying the influence of energy drinks in removing the smear layer and exposing dentinal tubules on root surfaces.Materials and Methods: Self-administered questionnaire were distributed to investigate the two most commonly consumed types of energy drinks in the community of Saudi Arabia then to study the effect of these two drinks on teeth structures. PH value were determined by pH –meter, titratable acidity was measured as described in A.O.A.C., total sugars and identity sugars were also detected by HPLC for the selected energy drinks. 72 teeth specimen were randomly distributed into 3 main groups. Code Red® and Bison® were evaluated, while distilled water was used as a control. Specimens were immersed for 5 minutes, 3 and 12 hours before micrographs were taken by the scanning electron microscope (SEM). Results: The most commonly consumed types of energy drinks among Saudi young adults were Code Red® and Bison® with a percentage of 68.6% and 37.8%, respectively. Both energy drinks tested were low-acid products, as indicated by comparatively low pH-values; (3.28 ± 0.036) and (2.72± 0.073) for Code Red® and Bison®, respectively. Also, both energy drinks have very low percent of total acidity. They recorded (0.057 ± 0.008) for Code Red® and (0.040 ± 0.006) for Bison®. However, the non-reducing sugars in Code Red® were markedly higher than in Bison® (6.95% in Code Red® vs. 0.97% in Bison®). Cod Red® hence showed stronger erosive potential on teeth surfaces and complete removal of smear layer at CEJ level when compared to Bison® as confirmed by the SEM results. No direct significant correlation between energy drinks consumption and teeth hypersensitivity had been proved, yet; significant results of associated teeth hypersensitivity was found in younger age group students who smoke during drinking energy drinks with a p-value of 0.006.
https://edj.journals.ekb.eg/article_75010_e80e36611d1b5f704597801c60953f72.pdf
2017-01-01
615
624
10.21608/edj.2017.75010
energy drinks
Erosion
Teeth Hypersensitivity
Scanning Electron Microscopy
Wahebah
Al Anazi
1
Department of Nutrition and Food Science, Faculty of Home Economics, University of Tabuk, Kingdom of Saudi Arabia.
AUTHOR
Ginat
Elsherif
2
Department of Food Science, Faculty of Home Economics , King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia and Agricultural Research Institute, Giza, Egypt.
AUTHOR
Eman
El Firt
3
Associate Professor of Periodontology, Faculty of Dentistry, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia and Faculty of Oral and Dental Medicine, Cairo University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
GENE EXPRESSION OF MIRNA-138 AND CYCLIN D1 IN ORAL LICHEN PLANUS
Objectives: To evaluate microRNA-138 (miR-138) gene expression and its target cyclin D1 (CCND1) gene and protein expression in oral lichen planus (OLP) mucosa in an attempt to investigate their possible roles in OLP immunopathogenesis. Methods: Sixty oral biopsy specimens were harvested from 30 healthy subjects and 30 OLP patients; subdivided into reticular, atrophic and erosive groups (n=10 each). Samples were subjected to quantitative real-time polymerase chain reaction analysis for quantification of miR-138 and CCND1 relative gene expression and immuohistochemical analysis to determine CCND1 protein expression. Results: Samples from OLP patients had a significant underexpression of miR-138 gene and overexpression of CCND1 at both gene and protein levels compared to normal mucosa samples. The lowest levels of miR-138 expression were observed in atrophic and erosive OLP compared to reticular OLP and the highest levels of CCND1 gene and protein expression was in atrophic OLP. An inverse correlation was demonstrated between the miR-138 expression and both CCND1 gene and protein expression in OLP patients. A significant positive correlation between CCND1 gene and protein expression was also observed. Conclusion: Down regulation of miR-138 increases the gene and protein expression of its potential target CCND1 in OLP mucosa which might have a pivotal role in the disease pathogenesis. Clinical relevance: This research implied that miR-138 may have a role in identification of symptomatic OLP lesions. MiR-138 might be considered as a potential tool in future OLP molecular therapy.
https://edj.journals.ekb.eg/article_75011_1f5672b5562796353b70bbfc176c6ca5.pdf
2017-01-01
625
638
10.21608/edj.2017.75011
Noha
Ghallab
noha26@yahoo.com
1
Associate Professor of Oral Medicine and Periodontology Faculty of Oral and Dental Medicine Cairo University
AUTHOR
Rehab
Kasem
2
Associate Professor of Oral Pathology Faculty of Oral & Dental Medicine Cairo University
AUTHOR
Safa
Abd El-Ghani
3
Associate Professor of Oral Pathology Faculty of Oral & Dental Medicine Cairo University
AUTHOR
Olfat
Shaker
4
Professor of Medical Biochemistry Faculty of Medicine, Cairo university
AUTHOR
ORIGINAL_ARTICLE
COMPARATIVE STUDY OF CALRETININ EXPRESSION IN ODONTOGENIC CYSTS AND TUMORS
Calretinin is a calcium-binding protein with molecular weight of 29 kDa (KiloDalton). It is expressed in a variety of normal and tumorigenic tissues. Its expression in odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. This study was undertaken to evaluate the expression of Calretinin in a group of odontogenic cysts and tumors. The expression of Calretinin was evaluated immunohistochemically in 42 samples including 8 cases of periapical cyst, 7 cases of dentigerous cyst, 7 cases of keratocystic odontogenic tumor, 8 cases of solid (multicystic) ameloblastoma, 6 cases of unicystic ameloblastoma and 6 cases of adenomatoid odontogenic tumor. Positive immunohistochemical reaction was found in all cases of solid ameloblastoma (100%), in 4 cases of unicystic ameloblastoma (67%) and 4 cases of keratocystic odontogenic tumors (57%) whereas none of the other odontogenic cysts and adenomatoid odontogenic tumors included in this study showed reactivity. Intensity was higher in the ameloblastomas compared with the keratocystic odontogenic tumors. On the basis of these results, it is suggested that calretinin might be used as a specific immunohistochemical marker for the ameloblastomas and could play an important role in the differentiation of aggressiveness of different odontogenic tumors.
https://edj.journals.ekb.eg/article_75012_95b6ea054c31d47c9ddc047cd061e11a.pdf
2017-01-01
639
645
10.21608/edj.2017.75012
Odontogenic cyst and tumors
Calretinin
Immunohistochemistry
Ashraf
Gaafar
1
Associate Professor of Oral and Dental Pathology, Faculty of Dental Medicine, Al-Azhar University (Assiut Branch).
AUTHOR
Khalid
Elrahawy
2
Associate Professor of Oral and Dental Pathology, Faculty of Dental Medicine, Al-Azhar University (Assiut Branch).
AUTHOR
Mohamed
Khalil
3
Lecturer of Oral and Dental Pathology, Faculty of Dental Medicine Al-Azhar University (Assiut Branch)
AUTHOR
ORIGINAL_ARTICLE
EVALUATION OF MULTIPLE ETIOLOGIC FACTORS RELATED TO BURNING MOUTH SYNDROME
This study was designed to evaluate the etiologic factors of Burning mouth syndrome and explore its characteristics with the specific outcome of increasing awareness of the condition as well as the pathogenesis. The study was performed on (60) patients of both sexes and different ages, from those (30) patients as a Group (A)were included which had a chief complaint of burning sensation and the remaining (30) patients were chosen free from any kind of pain or disease were considered as a Control Normal Group (B). All of these patients were evaluated by means of questionnaire, thorough Clinical Intra and Extra oral examinations and Salivary flow measurements were taken by schirmer tear strip test. The correlation between statistical analysis of both Groups (A&B) assured the findings of this study that Age-adjusted incidence was higher in women than Men as well as the Postmenopausal women was the highest disease incidence. Also; other factors as (tobacco, oral lesion, stress, problems with dentition, some food allergy) can be accelerated to severe conditions.
https://edj.journals.ekb.eg/article_75013_d41c6b51053427326bab3290a46aebfd.pdf
2017-01-01
647
655
10.21608/edj.2017.75013
Burning mouth syndrome
Diagnosis
Treatment
Etiology salivary flow measurements
Orofacial pain
Hala
Mahmoud
1
Associate Professor, Oral Radiology, Diagnosis & Oral Medicine Department, RAK College of Dental Sciences ; RAK Medical & Health Sciences University.
AUTHOR
Nadheera
awlaqi
2
Research Student in RAK College of Dental Sciences; RAK Medical & Health Sciences University.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF AN ANTI-HEPATITIS C VIRAL DRUG ON RAT SUBMANDIBULAR SALIVARY GLAND
Background: In the last 3 years, hepatitis C virus (HCV) treatment has undergone significant changes. Sofosbuvir is a new drug; it is a nucleotide analogue that is a highly potent inhibitor of the nonstructural protein 5B (NS5B) polymerase in HCV.Methodology: 30 adult male albino rats weighing about ±200 gm were used. The rats were divided into three groups 10 rats each. Group I(control group) received distilled water. Group II received sofosbuvir 40 mg/kg/day dissolved in distilled water for 45 days. Group III received sofosbuvir 40 mg/kg/day dissolved in distilled water for 90 days. All animals were sacrificed by ketamine overdose. The submandibular salivary glands were dissected. Specimens were examined histologically, immunohistochemically using anti- nuclear factor κ B antibody, histomorphomotry and statistically.Results: Group I showed normal histological structure for the ducts and acini however, group II showed acinar and ductal vacuolization, discontinuity of the epithelial lining with retained secretion in the excretory duct, and chronic inflammatory cell infiltration. These changes were accentuated in group III accompanied by acinar and ductal shrinkage. Immunohistochemical results of group І showed negative immunoreactivity for NF-κB in nuclei of the acini and ducts with very weak cytoplasmic reaction. However group II showed moderate nuclear and cytoplasmic immunoreactivity for NF-κB in the acinar and ductal cells. Regarding group III, the ductal and acinar cells exhibited strong immunoreaction in their nuclei and cytoplasm. Statistical results proved that the difference in the mean values were statistically highly significant between groups II & I, groups III & I and groups II & III.Conclusion: We concluded that sofosbuvir may affect the function of submandibular salivary gland through increased ROS levels in acinar and ductal cells leading to degenerative effect.
https://edj.journals.ekb.eg/article_75014_244b9ebc82ab2b38d862fa271a635b9f.pdf
2017-01-01
657
665
10.21608/edj.2017.75014
Sofosbuvir
Submandibular salivary gland
histological study
NF-κB
Zeinab
Salem
1
Lecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University
AUTHOR
Dalia
Elbaz
2
Assostant Professor, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University
AUTHOR
Dina
Farag
3
Lecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University
AUTHOR
ORIGINAL_ARTICLE
EXPRESSION OF MMP-1 AND MMP-9 IN LOCALIZED AGGRESSIVE PERIODONTITIS PATIENTS BEFORE AND AFTER TREATMENT: A CLINICAL AND IMMUNOHISTOCHEMICAL STUDY
Aim: This study aimed to clinically investigate the efficacy of β-glucan supplementation to non-surgical periodontal therapy in localized aggressive periodontitis (LAP) patients. In parallel, immunohistochemical detection of matrix metalloproteinase-1 (MMP-1) and MMP-9 expression levels has undergone to determine the subcellular changes behind the clinical status and reliability of each antibody in predicting the periodontal condition.Method: 40 subjects suffering from LAP were randomly and equally assigned to receive scaling and root planning (SRP); either with placebo pills (Group I) or β-glucan (100 mg/ once a day) (Group II), for 40 days. Subjects were clinically monitored on day 0 and day 91. Gingival samples were harvested from hopeless teeth sites to be investigated histologically and immunohistochemically using matrix metalloproteinase (MMP)-1 and 9 antibodies form each participating patient; at the same time intervals.Results: The experimental intervention showed a greater mean of probing pocket depth reduction (p=0.1128), with significant gain in clinical attachment (p=0.0180) and reduction of gingival inflammation (p=0.0207) compared to the control group at the end of the study. Furthermore, the experimental protocol was able to achieve better modulating effects on the levels of MMP-1 and MMP-9 compared to control therapy, along with enhancing protective healing patterns. MMP-9 was more sensitive indicator of the periodontal condition compared to MMP-1.Conclusions: The curre nt study demonstrated that the non-surgical treatment of LAP is markedly improved clinically by the adjunctive use of β-glucan, accompanied by a trend for modulation of the cytokine profile in gingival tissue samples with better healing events. The results also suggest that the expression of MMP-9 may be a precise indicator of periodontal disease activity.
https://edj.journals.ekb.eg/article_75015_484a610aadf794b8ff0ce89efa42b1e9.pdf
2017-01-01
667
684
10.21608/edj.2017.75015
β-glucan
localized aggressive periodontitis
matrix metalloproteinases
randomized double blind clinical trials
Hala
Hazzaa
1
Assistant Professor of Oral Medicine, Periodontology, Diagnosis and Radiology. . Faculty of Dental Medicine for Girls. Al Azhar University.
AUTHOR
Eman
Abo Hager
2
Assistant Professor of Oral and Dental Pathology. Faculty of Dental Medicine for Girls. Al Azhar University.
AUTHOR
ORIGINAL_ARTICLE
CORRELATION BETWEEN BONE DENSITY OBTAINED BY CBCT AND PRIMARY STABILITY OF SINGLE MIDLINE IMPLANT USED TO RETAIN MANDIBULAR OVER DENTURE IN ELDERLY EDENTULOUS PATIENTS
Introduction: Implant retained over-dentures are widely used for the rehabilitation of edentulous mandible to increase over-denture retention and stability. Implant stability can be measured by various non-invasive clinical test methods as resonance frequency analysis using Osstell. CBCT is widely used in dentistry specially in implant planning but without accurate information about bone density. However, with this increased usage of CBCT in dental field especially in implantology, the predictability of primary implant stability from CBCT image is of great value for oral implantologists.Methodology: Twenty two completely edentulous patients were included in our study. After performing the conventional steps of complete denture construction, the patients were imaged by CBCT using Planmeca ProMax® 3D Mid (Planmeca, Helsinki, Finland). A virtual implant was placed in the potential implant site in a position simulating the planned position of implant in the midline of mandible and the bone density in the potential implant site (inside) and 1mm around the implant (outside) was assessed. After implant installation, the primary stability was assessed using radiofrequency by the osstell device. Results: There was non-statistically significant direct correlation between bone density (for both inside and 1mm surrounding the potential implant site) and primary implant stability. Conclusion: Within the limitation of our study, no correlation was found between bone density measured from cone beam CT and primary stability measured by Osstell in elderly edentulous patients receiving single midline implant
https://edj.journals.ekb.eg/article_75016_b91f1db997ce6b9d38b69f6e7013b49c.pdf
2017-01-01
685
690
10.21608/edj.2017.75016
CBCT – primary implant stability
bone density
Ahmed
Abd Alsamad
1
Lecturer of Oral And Maxillofacial Radiology, Faculty of Oral and Dental Medicine, Cairo University.
AUTHOR
Ahmed
Fayyad
2
Lecturer of Prosthodontics, Faculty of Oral and Dental Medicine, Cairo University.
AUTHOR
ORIGINAL_ARTICLE
RADIOGRAPHIC EVALUATION OF THE EFFICACY OF WIDE DIAMETER IMPLANTS IN THE IMMEDIATE REPLACEMENT OF MANDIBULAR MOLARS
Background: Immediate implant placement to simultaneously replace mono-radicular teeth at the time of extraction has gained wide acceptance as a reliable and predictable treatment option. Application of this option in replacement of molars has presented a difficult challenge both surgically and prosthetically.Aim of the study: The current study aimed to investigate the efficacy of wide diameter implants in the immediate replacement of mandibular molars over a period of 24 months.Methods: Thirty implants were utilized in the immediate replacement of mandibular molars in a one stage surgical procedure following the atraumatic extraction of the teeth. The implants were restored after three months and followed-up at 12 and 24 months to evaluate the crestal bone loss around them.Results: A survival rate of 96.6% was evident at the end of the study period (24 months) with a mean crestal bone loss of 0.33-mm at 12 months and 0.19-mm at 24 months with a total mean crestal bone loss of 0.52-mm during the study period.Conclusion: Wide diameter implants are a reliable and predictable means to immediately replace mandibular molars with stable long-term prognosis with the consideration of proper extraction procedures and maintenance of the socket architecture.
https://edj.journals.ekb.eg/article_75017_719b6e538c9279d4bba1938786208b90.pdf
2017-01-01
691
697
10.21608/edj.2017.75017
Wide diameter implants
Immediate molar replacement
Immediate implant placement
Rania
Abdulmaguid
1
Lecturer at Oral Medicine and Periodontology Department, Faculty of Dentistry, MSA University.
AUTHOR
Ziad
Rabie
2
MSc Prosthodontics, Fellow ICOI, Private Practice, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
HISTOLOGICAL AND ULTRASTRUCTURAL EFFECT OF ZINC SULPHATE SUPPLEMENTATION ON THE ALVEOLAR BONE OF CALCIUM DEFICIENT RATS
Objective: The aim of the present study was to investigate the efficiency of zinc sulphate supplementation in treatment of the alveolar bone osteoporosis in rats induced by calcium deficient diet.Design: Thirty adult male Wistar rats with an average weight of 200-250 grams were randomized into 3 groups. Group Ι (control group): received standard rat chow, Group ΙΙ (osteoporotic group): received calcium deficient diet and Group ΙΙΙ (zinc treated group): received calcium deficient diet plus oral supplementation of zinc sulphate. After 3 months, the rats were sacrificed. The mandibles were examined histologically, histomorphometrically, ultrastructurally and by energy dispersive X ray microanalysis (EDX). Results: Histologically the impaired bone quality induced by calcium deficiency were significantly improved by zinc supplementation. The alveolar bone restored its structural organization in form of thick bone trabeculae, decrease number of osteoclasts and regularly distributed osteocytes. Histomorphometric analysis showed significant reduction in area percentage of alveolar bone trabeculae in osteoporotic group (p<0.001), while zinc treated group showed significant increase in the area percentage of bone compared to the osteoporotic group (p<0.001). Ultrastructurally, surface roughness and irregularity of the buccal cortical plate were observed in osteoporotic group. However, zinc treated group showed smooth and regular bone surface with minor irregularities. EDX microanalysis indicated a significant decrease in calcium content in osteoporotic group (p<0.001), while zinc treated group showed significant increase in the calcium percentage of bone compared to the osteoporotic group (p<0.001). Conclusions: In a rat model zinc supplementation prevents the bone loss induced by calcium deficient diet.
https://edj.journals.ekb.eg/article_75018_4031f00dd01fbb1f5161f6fa73ab3e73.pdf
2017-01-01
699
710
10.21608/edj.2017.75018
zinc
alveolar bone
Osteoporosis
Calcium deficiency
Dina
Nagui
1
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.
AUTHOR
Nesma
Khalil
2
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Egypt.
AUTHOR