2024-03-28T22:36:49Z
https://edj.journals.ekb.eg/?_action=export&rf=summon&issue=11447
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
The role of Platelet Rich Fibrin in bone defect regeneration
Mazen
Abou ElKhier
Laila
Amin
Nagla
Salama
Background: Natural tissue regeneration relies on a cocktail of signaling molecules and growth factors. During natural wound healing, activated platelets concentrate in the wound area and secrete factors that play a role in wound healing. Platelet rich fibrin (PRF) represents a revolutionary step in the platelet gel therapeutic concept. It needs only centrifugation of the natural blood without additives. Objectives: This present study designed to evaluate the role of PRF on regeneration of bone defect. Design: In this study 24New Zealand white male rabbits were used with bilateral cortical bony defects in the submental mandibular area, divided into two groups. The right bone defects were filled with PRF (experimental) and the left bone defects kept empty (control). Each group was equally subdivided into three subgroups; rabbits were sacrificed at 2nd, 4th and 6th weeks. Immunohistochemical analysis for collagen I and vascular endothelial growth factor (VEGF) were done. Results: The histological findings illustrated that all experimental subgroups showed more deposition of osteoid tissue and well organized bone trabeculae. Immunohistochemical findings of collagen I revealed statistically to be the highest in PRF group. While VEGF illustrated a positive expression by bone marrow stromal cells and bone tissue cells in different value and the PRF group recorded the highest value. Conclusion: It was concluded that PRF can accelerate bone regeneration and had effect on collagen I and VEGF expression.
Bone defect
Platelet rich fibrin
Collagen I
VEGF
2018
04
01
1161
1170
https://edj.journals.ekb.eg/article_77001_d127a4d2be4076b4e2e75096e2365cf3.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Reading Hounsfield Units from Direct Panoramic Images. A Software Technique
Shereen
Abdel Moula
Hussein
Saudi
The present research was conducted as a trial to find out a simple quick low radiation and less expensive methodology to read and display the HU units expressed by the CBCT from the direct panoramic images during planning for implant placement before the decision of CBCT. A specially developed small Visual Basic program was designed to load the panoramic images to read and display the mean value of the HU of the areas clicked on the panorama and display the bone category according to Misch classification. A total of 100 edentulous sites were explored for the mean value of bone density expressed in HU from CBCT using Simplant program and by the Visual Basic program from the digitized panoramic radiographs for the same site in every patient. Results showed no statistically significant difference were found between the mean value of the HU gained from the Simplant program and those gained from the visual basic program in all categories of bone density including D1, D2, D3 and D4. The software may be a promising tool that can help in prediction of bone density during the planning for implant placement.
2018
04
01
1171
1176
https://edj.journals.ekb.eg/article_77003_3857bfa2297db880b24c4fe57f379283.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
THE INTERRELATIONSHIP OF METABOLIC SYNDROME AND BEHCET’S DISEASE IN A COHORT OF EGYPTIAN PATIENTS (A cross-sectional study)
Samah
El Meadawy
Hesham
El Sharkawy
Azza
Elbayomy
The prevalence of metabolic syndrome (Met S) increased in chronic inflammatory diseases such as systemic lupus erythematosus and rheumatoid arthritis. Behçet’s disease (BD) is a chronic, multisystem disease with immuno-inflammatory etioloy. The study was aimed to assess the prevalence of Met S among Egyptian Behcet patients. We also tried to detect the relationship of Met S and its various aspects with activity and severity of BD and oral ulcer activity. The study population consisted of 60 BD patients and 60 healthy, age and sex matched controls. BD patients were diagnosed according to the criteria of the International Study Group of Behçet’s disease. Clinical data were collected at the time of enrollment as body mass index (BMI), waist circumference (WC), and arterial blood pressure. Total cholesterol (TC), low-density lipoprotein (LDL-c), high density lipoprotein (HDL-c), triglycerides (TGs), and C-reactive protein (CRP) were measured. Fasting plasma glucose (FPG), fasting insulin, and the homeostasis model assessment of insulin resistance (HOMA-IR) were estimated. Met S diagnosis was established according to the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) criteria. The prevalence of Met S in sixty BD patients was 30%, while in the healthy, age and sex matched control group was 16.6 % (p>0.05). Among males, Met S prevalence was 28.5% in BD patients and 9.3% in controls, while in females, Met S prevalence was 33.3% in Behçet patients and 35.9% in controls. The BD severity and activity as well as oral ulcer activity were negatively correlated with the duration of the disease and patients’ age (p<0.5). The disease severity and activity were positively correlated with fasting insulin, HOMA-IR, TC, TGs, LDL-c, IL-6, and hsCRP, while oral ulcer activity was positively correlated with insulin and TGs (p<0.05). It was concluded within the limits of this study that BD patients should be followed up regularly for diabetes mellitus, hyperlipidemia, and hypertension to prevent Met S development.
Behcet disease
Metabolic syndrome
Lipid profile
Insulin Resistance
2018
04
01
1177
1186
https://edj.journals.ekb.eg/article_77004_3dba339b18cb423885c45b782892018b.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Prognostic significance of fascin and Wilm’s tumor 1 (WT1) in oral squamous cell carcinomas – case control study
Samah
El-Meadawy
Naglaa
Salama
Lobna
Radwan
Mahmoud
El-sherbiny
Aims: To elucidate the role of fascin and WT1 expression in oral squamous cell carcinoma (OSCC) by correlation with clinicopathological parameters.Subjects and methods: Paraffin sections of 27 OSCC tissue were immunohistochemically stained with fascin and WT1 using the avidin-biotin-peroxidase staining method. Correlations between fascin and WT1 and various clinicopathological features, and prognosis were studied.Results: Immunohistochemical study revealed significant increase of fascin and WT1 in OSCC in relation to control group (p< 0.000). Receiver operating characteristic (ROC) curve for fascin and WT1 were conducted for detection of recurrence. Fascin showed fair area under curve (AUC) (AUC=0.66), with sensitivity of 63.6 % and specificity of 50.0 % at cutoff value of 42.5 %. WT1 also showed fair AUC (AUC=0.63), with sensitivity of 63.6 % and specificity 68.8 % at cutoff value of 40.0 %. ROC curve for WT1 and fascin were conducted for detection of lymph node infiltration. While fascin showed excellent AUC (AUC=0.865), cutoff value of 52.5 %, with sensitivity of 70.0 % and specificity of 94.1%. WT1 also showed excellent AUC (AUC=0.791), cutoff value of 42.2 %, with sensitivity of 70.0 % and specificity 70.6%. Significant associations were detected between median fascin cutoff value at 45% and well differentiated tumor (P=0.04), T3&T4 tumor size (P= 0.01), LN infiltration (P=0.05), and tumor-node metastasis 3 and 4 (TNM3 &TNM4) staging (P=0.02). Significant associations were found between the median cutoff value of WT1 and moderately differentiated tumor (P=0.02), and LN infiltration (P=0.01). The 2-year survival rate of patients with fascin of ≥ 45% and WT1 ≥ 40% were nonsignificantly higher than that of patients with fascin of < 45% and WT1 <40% (P=0.09, P=0.55). Univariate analysis demonstrated that Fascin, WT1 were significant risk factors of LN (p=0.005, 0.02) but not considered as risk factors of tumor recurrence. Conclusion: Fascin and WT1 have oncogenic effects playing an important role in progression of OSCC. Overexpression of them contributes to a more aggressive clinical course. Understanding their role on OSCC and other tumors will facilitate the development of new treatment strategies.
Oral squamous cell carcinoma
fascin
Wilm’s tumor 1 (WT1)
tumor markers
2018
04
01
1187
1202
https://edj.journals.ekb.eg/article_77007_ae091be14177fc8b1d47e42fce21174f.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Silymarin: Adjunctive Treatment in Hepatitis C Associated Oral Lichen Planus
Malak
Shoukheba
Shereen
Ali
Recently, lichen planus has been reported to occur with chronic hepatitis C (HCV). This study was conducted to evaluate the effect of silymarin liver support as an adjunctive to local triamcinolone acetonide orabase in the treatment of atrophic/erosive oral lichen planus in hepatitis C positive patients. Methods: Twenty HCV patients with erosive lichen planus were divided into two groups, 10 patients each: Group I was treated with topical triamicinolone acetonide in orabase, group II was treated with topical triamicinolone acetonide in orabase and systemic silymarin 140 mg capsule 3 times daily for 3 months. Each patient was examined at baseline, 2, weeks and 1, 2 and 4 months of therapy. Pain was scored using the visual analogue scale. Serum level of TNF-alpha (TNF-α), AST and ALT were estimated at baseline 1, 2 and 4 months’ evaluation periods. Results: Significant decrease in pain score was observed in both groups and there was a statistically significant difference between them at all treatment periods in favor to group II. Additionally, both groups showed a decrease in the size of lesions which was greater in group II compared to the control group. A significant reduction of the mean TNF-α continued up to 6 months in both groups with statistically insignificant difference between them at one month, while statistically significant difference in favor to group II at 2 and 4 months was observed. Conclusion: The combination of topical triamicinlone and systemic silymarin could be suggested as a promising therapeutic alternative to systemic corticosteroid for hepatitis C virus associated oral lichen planus, and immunocompromised patients.
Hepatitis C virus
Oral lichen planus
TNF-α
2018
04
01
1203
1213
https://edj.journals.ekb.eg/article_77374_3b4f59ed56215003e1356b8b18de7b8f.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
The Effect of Voxel Size on the Reliability and Reproducibility of Alveolar Bone Crest Identification on Cone Beam CT Scans
Nora
Saif
Amr
El-Beialy
Objective: the aim of this study is to compare the reliability and reproducibility of landmarks representing the labial alveolar bone crest of maxillary and mandibular teeth using two different CBCT machines with different image resolutions and voxel size and accordingly different radiation dose. Material & Methods: 340 landmarks representing the alveolar bone crest opposite each tooth from the central incisor up to the second molar on sixteen CBCT data sets were identified. The landmarks were located by the principle operator, and relocated by the same principle operator to evaluate the intra-observer reproducibility, then located by the second observer to evaluate the inter-observer reliability error. For testing the intra-observer reproducibility and inter-observer reliability of the landmark identification within each scanner data, the Mean Absolute error (MAE) and the Dahlberg error (DE) and Intra-class correlation coefficient tests with 95% confidence intervals were used. Independent samples t-test was used to compare the intra-observer and inter-observer error between the two scanners. Results: The Mean Absolute Error (MAE), the Dahlberg Error (DE) and the Intra-Class Coefficient (ICC) tests showed excellent intra-observer reproducibility values in the x, y and z-axis for the Galileos scanner, with the least reproducibility in the z-axis. The same concordance was found for the Planmeca CBCT scanner, with excellent reliability in the x, y and z-axis, and the least reproducibility in the z-axis. For the inter-observer reliability testing, the same pattern was found for each CBCT scanner. Very good inter-observer reliability was found in the x, and y dimensions, while the least was found in the vertical z-dimension. Conclusion: The Galileos and the Planmeca CBCT scanners produced comparable reliability and reproducibility for identification of alveolar bone crest landmarks. The vertical axes showed the least accurate coordinates for landmarks identification.
2018
04
01
1215
1226
https://edj.journals.ekb.eg/article_77375_528434a07e9bef4e28ad46ca49062348.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Evaluation of Fosfomycin and Laser in the Treatment of Methicillin-Resistant Staphylococcus Aureus (MRSA) Osteomyelitis in Rabbits
Walaa
Abdel-Fatah
Raghda
Hager
Aim: to evaluate the effectiveness of fosfomycin and Diode laser treatment in experimental osteomyelitis induced by MRSA in rabbits.Methods: Osteomylitis Infection was induced in the tibiae of thirty rabbits by the injection of a bacterial inoculum (7 x 106 CFU/ml) of a clinical MRSA isolate two weeks after infection, each animal was assigned to a treatment group: Group ( 1) include 10 animals were left untreated Group (2); Fosfomycin monotherapy at 75 mg/kg of body weight once daily (n_10), Group 3: (n = 10) rabbits were treated for 2 weeks with Diode laser 940 nm .After a 2-week treatment period, the animals were euthanized, and the infected tibiae were processed for quantitative bacterial cultures and bone density measurement . RESULTS: bone culture from bone was positive for MRSA in all groups with no significant difference (p value <0.302). Based on bone density measurement; bone density was higher level at laser group followed by antibiotic FOF group then control group but there is no with significant difference p value Conclusion: The difference between laser group and fosfomycin group not significantly different between the two methods for modeling rabbit. However, histopathological method is recommended because it has certain increase confirmation which line of treatment was viald.
Rabbit
MRSA
Osteomylitis
bone density
Diode laser
Digora and Fosfomycin
2018
04
01
1227
1233
https://edj.journals.ekb.eg/article_77376_17f8412eae9bc324cd7436b8bdc70d25.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Relation between salivary cortisol and alpha amylase levels and anxiety in Egyptian patients with minor recurrent aphthous stomatitis
Mai
Zakaria
Ragaa
Hosny
Background/Aim: Recurrent aphthous stomatitis (RAS) is the most prevalent ulcerative disorder of the oral mucous membrane. The aim of the present contemplate is to assess salivary cortisol (SC) and salivary alpha amylase enzyme (SAA) levels in relation to anxiety in Egyptian patients with minor recurrent aphthous stomatitis. Subjects and Methods: patients with minor RAS as a study group (RAS group, n = 35) and healthy subjects as a control (control group, n = 35) who were matching regarding age, sex, and socioeconomic state were included in this study. SC and SAA were assessed in all participants using enzyme-linked immunosorbent assay (ELISA) and kinetic enzyme assay respectively. Hamilton’s anxiety scale (HAS) has been used for evaluation of anxiety level in both study groups.Results: A significantly high mean SC (1.7 ± 0.34 μg/dl), SAA (137.87 ± 47.16 U/ml) levels and anxiety score (26.7 ± 2.21) were recorded in RAS group (p = 0.00, 0.02, 0.00) respectively in comparison to control group.Conclusion: Anxiety may have a pivotal role in etiopathogensis of RAS hence; Psychological management should be considered in RAS treatment.
Recurrent aphthous stomatitis
saliva
Cortisol
alpha amylase
anxiety
2018
04
01
1235
1243
https://edj.journals.ekb.eg/article_77378_19f6e9ae80e51d15ba47ac71d6a8dd73.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Isolation, Characterization and Osteogenic differentiation potential of periodontal ligament stem cells (in vitro study)
Marwa
Moussa
Marwa
Abd El Hameed
Dina
Sabry
Background: Stem cells are unspecialized and immature cells that have the potential to develop into many different cell lineages via differentiation in a suitable culture media. Periodontal ligament stem cells (PDLSCs), which reside within the perivascular space periodontium, possess characteristics of mesenchymal stem cells and are a promising tool for periodontium regeneration.Purpose: This study aimed to isolate and characterize periodontal ligament stem cells (PDLSCs) from albino rat, Identification of surface markers expression utilizing flow cytometric analysis (FACS) and assessment of the osteogenic differentiation capability of these cells.Material and methods: PDLSCs were collected from the root surfaces of mandibular 1st molars from 6 adult male albino rats. Collected samples were enzymatically treated and centrifuged to allow cells release. Cells were cultured for fourteen days. After reaching confluence, the isolated cells were characterized by flow cytometry using CD73, CD133 and C-Kit. The cells were induced for osteogenic differentiation by placing them in osteogenic culture media. Osteogenic differentiation was evaluated by Alizarin Red stain and by Molecular assessment for Bone sialoprotein (BSP) and alkaline phosphatase (ALP) genes expression by real time PCR (q RT-PCR). Results: The results showed successful isolation of stem cells from periodontal ligament. PLSCs were identified as mesenchymal stem cells by expression of mesenchymal stem cell markers through FACS. PDLSCs showed positive expression to CD73 marker and negative expression to hematopoietic markers CD133 and C-kit. Our results showed successful differentiation of PDLSCs into osteogenic lineage. The formed mineralized matrix stained by alizarin red and was more advanced at 21 days after osteoblastic differentiation. Real Time PCR (q RT-PCR) for quantitative expression of BSP and ALP showed significant increase in the expression of BSP and ALP from day 14 to day 21 in differentiated PDLSCs.Conclusion: According to the obtained results, we conclude that PDLSCs could be successively isolated from periodontal ligament tissues. Isolated PDLSCs possess an osteogenic potential and show differentiation into osteogenic lineage when cultured in suitable osteogenic medium.
Periodontal ligament stem cells (PLSCs)
CD133
C-Kit
CD73
Osteogenic differentiation
BSP
ALP
2018
04
01
1245
1254
https://edj.journals.ekb.eg/article_77379_7e2edfeee3d7390536df7eb82745b2a2.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Detection of Mmp-8 expression in nanosilver-treated and tetracycline-treated periodontitis induced in albino rats
Ghada
Abdel-Latif
Wafaa
El-Hossary
Objective: This study aimed to evaluate the histopathological expression of Mmp-8 in the experimentally-induced periodontitis treated with tetracycline, and nanosilver in albino rats.Material and Methods: fifty five male albino rats were included in this study. On the first day, 5 rats were euthanized as healthy group for histological characteristics of interdental alveolar bone between the maxillary incisors. Fifty rats were anesthetized; elastic rings were placed around the cervix of the right maxillary incisors for 7 days to induce periodontitis. On the eighth day, the ligatures were removed, five rats were euthanized before any treatment (day zero) and served as positive control group. The remaining 45 rats were randomly assigned to three groups as following: Group I; 15 rats did not receive any treatment. Group II; 15 rats treated locally with tetracycline hydrochloride (50 mg /mL) daily. Group III; 15 rats treated locally with nanosilver (12.5μg /mL) daily. Five animals from each group (I, II and III) were euthanized at day 7, 15 and 30 of day zero. The area of the maxillary incisors was decalcified and processed for hematoxylin and eosin stain and immunohistochemical stain for Mmp-8. Results: Local application of nanosilver in experimentally-induced periodontitis in rats exhibited tendency for bone formation from day 7 with externalization of the inflammation compared to local application of tetracycline. The tetracycline treatment demonstrated tendency for localization of inflammation detected as abscess formation. The tendency for new bone formation was detected after 15 days of treatment. Mmp-8 expression was strongly expressed after the induction of periodontitis. In nanosilver treated group, Mmp-8 started to fade within the connective tissue from day 15 and was detected only on the surface epithelium. In tetracycline group, Mmp-8 was detected at day 15 surrounding the abscesses then it completely disappeared at day 30.Conclusion: Mmp-8 is intensely expressed in inflamed periodontal tissue. This expression fades earlier with the nanosilver treatment compared to the tetracycline treatment. The non-inflamed periodontal tissues did not express Mmp-8.
nanosilver
tetracycline
Rats
matrix metalloproteinase-8
experimentally-induced periodontitis
Immunohistochemistry
2018
04
01
1255
1264
https://edj.journals.ekb.eg/article_77380_6ce217bbdaf8c815d380b690fd763350.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Chemopreventive potential of Spirulina platensis in tongue carcinogenesis induced by 4-nitroquinoline-1-oxide in rats (histological and immunohistochemical study)
Elham
Mahmoud
Tahany
Haggag
Objective: The utility of natural, synthetic, or biological chemical factors that hide or inhibit carcinogenic incidence is the aim of cancer chemoprevention. One of the food items that play an important role in cancer chemoprevention is Spirulina platensis (blue green alga). Until now, there is relatively little information on the cancer chemopreventive role of Spirulina platensis (Sp) in animal researches. So, the squamous cell carcinoma can be induced by using 4-nitroquinoline-1 oxide (4-NQO) in tongue mucosa of rats and the chemopreventive efficiency of Spirulina platensis regarding the oral carcinogenesis was examined. Materials and methods: 30 healthy albino rats were equally divided into 3 groups. Group I (rats received normal saline) served as control, Rats of both group II and III received 50ppm 4NQO daily in drinking water for 20 weeks. Spirulina platensis was orally administered to rats of group III at a dose of 300 mg/kg body weight, once daily via gastric tube. After 20 weeks of drugs administrations, the histopathological changes were evaluated microscopically. Furthermore, by using the image analyser computer system, the anticarcinogenic potential of Spirulina platensis was examined through the immunohistochemical expressions of cytokeratin 5 in tongue specimens.Results: oral administration of Spirulina platensis considerably reduced the frequency of 4-NQO produced tongue carcinogenesis. A lower percentage of cytokeratin5 immunoexpression was registered in the Spirulina platensis treated group when compared to the carcinogenic group.Conclusion: our results demonstrated that Spirulina platensis can exert chemopreventive effect against tongue carcinogenesis through suppression of cell propagation and promotion of cell adherence.
Tongue carcinogenesis
4-Nitroquinoline-1-oxide
Chemoprevention
Spirulina platensis
Rats
2018
04
01
1265
1274
https://edj.journals.ekb.eg/article_77381_447773acbb00aa07e5c3888b8f3b6cf5.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Histological changes of tongue papillae induced by the artificial sweetener Aspartame and the protective effect of Platelet-rich plasma
Dalia
Zahran
Elham
Mahmoud
Mahmoud
Mahmoud
Aspartame (ASP) is one of the most controversial food additives found in various food products all over the world. Once ingested, ASP is metabolized to phenylalanine, aspartic acid, and methanol. The aims of the study are to evaluate the effects of ASP administration on the dorsal surface of rats’ tongues and to investigate the possible protective effect of Platelet-rich plasma (PRP). Thirty adult male albino rats were used and divided into three equal groups. The control group received the vehicle only. ASP group received a dose of 250 mg/kg b wt for four weeks while the PRP group were treated as ASP group, in addition they received a single local injection with PRP in the right lateral border of the tongue. The most obvious changes were loss of normal conical appearance with hyperkeratosis of filiform papillae. The epithelial cells showed hyperplasia (acanthosis), cellular pleomorphism and nuclear hyperchromatism. Distorted and atrophied fungiform papilla with degeneration of epithelial cells and pyknotic nuclei were visible. In the lamina propria numerous dilated and congested blood vessels were obvious. SEM examination revealed disfigurement of tongue papillae. The fungiform papillae showed irregular wrinkled surface and ill-defined taste pore. PRP treatment almost restored the normal architecture of tongue papillae. Chronic aspartame ingestion could result in marked morphological alteration of dorsal surface of the tongue and PRP exerted a protective role against this effect.
Aspartame
platelet-rich plasma
tongue papillae
rat
2018
04
01
1275
1282
https://edj.journals.ekb.eg/article_77382_962d6918a034b857dfe11e5b053a2bd8.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Evaluation of Root Canal Configuration of Permanent Mandibular Anterior Teeth in Egyptian Subpopulation: A Cone Beam Computed Tomography Study
Soha
Basha
Cone beam computed tomography (CBCT) affords high-quality image with a relatively low radiation doses and low costs. It is considered as a valuable tool in endodontic field as it determines root morphology, number of roots, canals and accessory canals. The aim of the present study was to evaluate the permanent mandibular anterior teeth canal morphology in Egyptian subpopulation. Materials and Methods: A total of 100 patients were enrolled in this study and were assigned in two groups (50 females and 50 males). In each group centrals, laterals and canines of both sides were examined (100 centrals, 100 laterals and 100 canines). CBCT Images were obtained using J Morita CBCT unit and canals were categorized into the five types of Vertucci’s classification. The observer evaluated the images twice with one-week interval between assessments. Results: Type I Vertucci configuration was the most prevalent configuration in our sample and the incidence of this type was slightly higher in female than male samples. Furthermore, type III and V Vertucci configuration were slightly more in males than females. Conclusion: In the present study it was concluded that the root and root canal morphology of Egyptian subpopulation was, in general, comparable to that of other populations and that the incidence of canal variations might occur as a result of ethnicity more than gender.
Apical foramen
CBCT
Root canal configuration
Vertucci
2018
04
01
1283
1291
https://edj.journals.ekb.eg/article_77383_55611bc5de987fef8e231e4ea694a49a.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Effects of Moringa Oleifera Aqueous Leaf Extract on Submandibular Salivary Glands of Diabetic Albino Rats
Radwa
El-sharkawy
Hala
El-kammar
Raneem
Obeid
Abdelfattah
Bdelkhalek
Moringa Oleifera (MO), also known as the ‘drumstick tree,’ is recognized as a nutritious and cheap source of phytochemicals, that a have a prominent anti-oxidative effect. Salivary gland dysfunction has been frequently reported in diabetic patients. Diabetes is a chronic metabolic disease that has complications mainly resulting from persistent hyperglycemia. Aim of the study: To Assess the effect of MO aqueous leaf extract on blood glucose levels in diabetic albino rats and its effect on submandibular salivary glands of diabetic albino rats. Materials and methods: the study comprised three groups; control, diabetic and MO treated groups. The experiment was terminated after fourteen days. The evaluation was performed by measuring the blood glucose levels and weight. Histological evaluation was done by H&E, PAS and IHC for COX-2. Results: the blood glucose levels and histological signs of diabetic complications were significantly lowered in the MO treated group. Conclusion: MO is a promising anti-diabetic treatment and may even reverse some of the diabetic complications.
COX-2
diabetes
Moringa oleifera
Reactive oxygen species
Submandibular salivary gland
2018
04
01
1293
1303
https://edj.journals.ekb.eg/article_77385_b297894139550204e0423263130fc4f9.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Correlation between Gingival Biotype, Marginal Bone Loss, Periapical Infection and Schneiderian Membrane Thickness in Chronic Periodontitis Patients (A Cross-Sectional
Ola
Ezzatt
Fatma
El Demerdash
Mostafa
Ashmawy
Background: Identification of factors affecting maxillary sinus membrane thickness would be of practical importance in surgical planning for both implant placement and otolaryngeal surgery. This study aimed to analyze a possible correlation between Schneiderian membrane thickness (SMT) and gingival biotype (GB), marginal bone loss (MBL) and presence of periapical infection (PAI) of related teeth using Cone beam computed tomography (CBCT)Subjects and Methods: A total of fifty consecutive moderate to severe chronic periodontitis patients were included in this cross-sectional study. The patients’ sex and age were recorded. Clinical measurement of (GB) was performed in the premolar and first molar area, and then (CBCT) images of patients were examined for (SMT), (MBL) and presence of (PAI) in the same area. All measured parameters were analyzed and graded. Then correlation between these parameters and Schneiderian membrane thickness was evaluated.Results: Higher percentage of severe SMT was observed in patient with thick gingival biotype (37.3%) than those with thin biotype (33.3%) and a direct positive correlation between SMT and MBL (r= 0.595, p<0.001) was detected. Cases with PAI showed higher mean sinus membrane thickness (5.3±1.97) than normal cases (2.26±1.90) (p<0.001).Conclusion: The presence of periapical infection marginal bone loss or clinically detected thick gingival biotype in the maxilla could be associated with thicker Schneiderian membrane, so clinicians should be aware of these clinical and radiographic findings that could predict the sinus membrane thickness.
CBCT
schneiderian membrane
gingival biotype
marginal bone loss
periapical infection
2018
04
01
1305
1313
https://edj.journals.ekb.eg/article_77386_c8fabf3c2907410252e944410e51d6f9.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
EVALUATION OF DOSE DEPENDENT EFFECTS OF GAMMA RADIATION ON RAT TONGUE INTERNAL STRUCTURES: HISTOMORPHOMETRIC AND IMMUNOHISTOCHEMICAL STUDY
Khaled
El-Haddad
Hamdy
Metwaly
Introduction: Radiotherapy is one of the common modalities in management of head and neck cancers. However, many injurious effects were reported on unavoidable oral tissues in the radiation field including the tongue and its heterogeneous internal structure. It is important to define the optimum dose of radiation with minimal hazards on the healthy tissues along with classification of the tissues according to their radio-sensitivity. Purpose: To evaluate histomorphometrically and immunohistochemically the dose dependent response of lingual internal structures of rats exposed to gamma radiation. Materials & Methods: Twenty-two adult male albino rats were divided into 3 groups: control group (6 rats), R1 group (8 rats) irradiated by 2.0 Gy whole-body gamma dose, and R2 group (8 rats) irradiated by 6.0 Gy. The effects on lingual internal structures were investigated after 3 days by histomorphometric and immunohistochemical staining for Caspase-3 using light microscopy. Results: There were a dose related changes in the minor salivary glands with more changes in serous glands. Mild changes were observed in muscles only at 6 Gy dose. The expression percentage of caspase-3 protein was significantly increased in a dose dependent manner in both of the irradiated groups when compared with control group P0.05. Conclusion: Gamma radiation especially 6 Gy dose adversely affects the internal structures of the tongue with the most affected are serous acini and the least are the muscle cells.
gamma radiation
Caspase-3
lingual glands
intrinsic muscles
2018
04
01
1315
1325
https://edj.journals.ekb.eg/article_77388_09a4eff9378e3a9299e22d8366cef75b.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Potential Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Zymogen Granules and Rab3D in Parotid Salivary Glands of Senile Albino Rats
Rabab
Hassan
Amany
Rabea
Background: Age-related changes of salivary glands affect their functions and structure. Rab3D is localized to zymogen granules (ZGs) and plays a critical role in secretory granules maturation and exocytosis. Nowadays, application of life sciences is used in tissue regeneration. Objective: The current study was designed to explore the possible effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on the function and morphology of ZGs in parotid glands of senile rats.Design: Fourteen clinically healthy male albino rats were used. Seven adult rats aged 6-8months were named as Control adult (Group I). The remaining seven rats aged 18-20 months (old age). For each old age rat the parotid gland of the left side represented the Old age untreated (Group II) and injected by 0.2 ml phosphate buffered saline (PBS). While, the parotid gland of the right side represented the Old age treated by stem cells (Group III) and injected by 1-1.5 x 106 BM-MSCs in 0.2 ml PBS. At the end of the experimental period (after four weeks), all the rats were euthanized and the parotid salivary glands were dissected out. The sections were prepared for histological, immunohistochemical and ultra-structural examinations in addition to real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. Statistical analysis: Data obtained from histomorphometric analysis and qRT-PCR assay were statistically described in terms of mean ± standard deviation (± SD).Results: Histological examination of Group I showed normal histological features of the terminal secretory units. Group II revealed that most of the acini were distorted and showed fatty degeneration. The oncocytes were noticeable. Group III illustrated more or less normal histological features. Immuno-expression of Rab3D was strong in Group I and Group III but mild in Group II. The ultra-structural examination of Group I showed apparently large and spherical ZGs. Group II revealed apparently small and ellipsoid ZGs. Lamellated lipofuscin granules were observed. Group III demonstrated obviously large and well defined spherical ZGs. The statistical results revealed that the highest means of Rab3D immunoreactivity area % and relative Rab3D gene expression level were demonstrated in Group I, followed by Group III, and the least values were for Group II. Conclusions: Allogeneic BM-MSCs have positive effect on age-related changes of parotid salivary glands by restoring the terminal secretory units’ structure, Rab3D expression as well as ZGs structure and function.
BM-MSCs
aging
Zymogen granules
Rab3D
Parotid salivary gland
2018
04
01
1327
1343
https://edj.journals.ekb.eg/article_77391_9ac169ae6e022e64a817175be392210e.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Non Surgical Treatment Of Chronic Periodontitis By Two Strategies Of Full Mouth Disinfection
Ahmed
Hamed
Introduction: Recent studies reported additional clinical improvement when moderate chronic periodontitis was treated by means of a one stage full mouth disinfection instead of standard treatment .The one stage full mouth disinfection procedure involves periodontal debridement of all pockets within 24 hours with application of an irrigation to all intraoral niches such as periodontal pockets and tongue dorsum. Aim : The aim of this randomized clinical trial was to assess the clinical improvement of chronic moderate periodontitis after full mouth disinfection compared to periodontal debridement alone .Subjects and methods: Thirty patients ( 18 males and 12 females ) suffering from moderate chronic periodontitis, free from any systemic disorders were recruited from the outpatient clinic, Faculty of Dentistry - Assiut University and presented at the department of Periodontology for assessment and treatment. Patients were assigned to the following groups: Group I: patients received scaling and root planing. Group II: patients received a full mouth disinfection including a full mouth scaling and root planing in combination with a disinfection of all intraoral niches by means of chlorhexidine. Group III: patients received a full mouth disinfection including a full mouth scaling and root planing in combination with a disinfection of all intraoral niches by means of tetracycline. Randomization of the patients and blinding of assessment were performed to avoid bias. Statistical analysis was performed with IBM SPSS statistics version 20 for windows. Results: Pocket reduction mean of group I was (+-1.8mm), gain of attachment mean for group I was (+-2.7mm) , Pocket reduction mean of group II was (+-1.2mm), gain of attachment mean for group II was (+-2.5mm), Pocket reduction mean of group III was (+-1.1mm), gain of attachment mean for group III was (+-2.3mm)Conclusion : All three treatment modalities showed clinical improvement. Full mouth disinfection either by chlorhexidine or tetracycline gave better clinical results than regular periodontal debridement but there were no statistically significant differences.
2018
04
01
1345
1351
https://edj.journals.ekb.eg/article_77392_7e50ac335ecf06738f49c8744ab05e80.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Evaluation of the role of YKL-40 and interleukin-8 (IL-8) as biomarkers for malignant transformation in oral lichen planus
Eman
Amr
Amal
Hussine
Rasha
Mostafa
Olfat
Shaker
Objective: This investigation was conducted to measure the levels of serum and salivary YKL- 40 and IL-8 in patients suffering from OLP and OSCC to assess their potential role as biomarkers of oral cancer. Methods: Forty five subjects with age ranging from 30 to 70 years were distributed into 3 groups; 15 patients having atrophic and/or erosive OLP, 15 patients suffering from OSCC and 15 systemically healthy control individuals.YKL-40 and IL-8 were identified in serum and saliva samples utilizing enzyme-linked immunosorbent assays. Results: Serum YKL-40 and salivary levels of YKL-40 and IL-8 in patients with OSCC and OLP were significantly higher than the healthy control group. In order to differentiate patients having OLP from those suffering from OSCC, our study utilized the analysis of the receiver operating curve (ROC) which showed an AUC of 0.878, 0.789, 0.789 and 0.7 for serum YKL-40, salivary YKL-40, serum IL-8, and salivary IL-8, respectively. Serum YKL-40 had the highest diagnostic accuracy revealing 93 % sensitivity and 73 % specificity. That has been followed by salivary YKL-40 and serum IL-8, both revealing a good diagnostic accuracy with 93%, 80 % sensitivity, and 60%, 73.3 % specificity respectively. Salivary IL- 8 as well yielded a good diagnostic accuracy and revealed degrees of sensitivity (80%) and specificity (63%). Cut off values of the biomarkers under investigation has been selected according to the distribution of sensitivities and specificities Conclusion: YKL-40 and IL-8 might be counted as biomarkers for identifying cancerous changes of OLP and the initial stage of OSCC.
Oral lichen planus
Oral squamous cell carcinoma
YKL-40
IL-8. Serum
saliva
2018
04
01
1353
1364
https://edj.journals.ekb.eg/article_77393_21ae5e9805e71f09a38307c93697b483.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
EFFECT OF INTRA-MASSETERIC BOTULINUM NEUROTOXIN INJECTION ON RAT PAROTID GLAND: HISTOMORPHOMETRIC, HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL STUDY
Khaled
El-Haddad
Introduction: Botulinum neurotoxin “BoNT” is a potent toxin which causes muscle paralysis. However, it is widely used in management of muscle-related disorders such as masseter hypertrophy. Intra-masseteric BoNT injection may predispose certain adverse effects on the adjacent tissues including parotid gland. The exact mechanisms as well as the reversibility of these effects on parotid glands still have been not fully elucidated. Purpose: To assess, histologically, histochemically and immunohistochemically, the effects of intra-masseteric botulinum neurotoxin injection on the parotid glands of albino rats. Materials & Methods: Fifteen adult male albino rats were divided into 2 groups: control group (5 rats received intra-masseteric injection of sodium chloride), and BoNT group (10 rats received intra-masseteric injection of 2.5 units BoNT). The latter group was divided equally into 2 subgroups according to the time of rats’ scarification (3 days and 14 days after injection). The parotid glands were studied by histomorphometric analysis, Periodic Acid Schiff reaction and immunohistochemical expression of neuron specific enolase marker. Results: BoNT injected rats showed acinar vaculations and decreased size, blood vessels congestion and mild fibrosis in both durations. 14 days subgroup displayed inflammatory cell infiltrate. BoNT group showed less intense reaction to PAS was observed in addition to under-expression of neuron specific enolase which was more severe in the rats sacrificed after 3 days. Conclusion: BoNT has an adverse effect on parenchymal and connective tissue elements of parotid salivary gland which is slightly modified by time factor.
Botulinum toxin
parotid gland
PAS
Neuron specific enolase
2018
04
01
1365
1376
https://edj.journals.ekb.eg/article_77394_e73f3b3095c31cfd489c065096080a3e.pdf
Egyptian Dental Journal
0070-9484
0070-9484
2018
64
Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
Keratin 10 In Investigating The Healing Power Of Bone Marrow Derived Stem Cells Compare d To Microvesicles In Tongue Of Irradiated Albino Rats
Dalia
Elbaz
Dina
Farag
Tahany
Haggag
Background: Multiple oral complications were reported in patients who receive radiotherapy for head and neck cancers. Objectives: Investigating the healing power of bone marrow derived mesenchymal stem cells compared to microvesicles on dorsal lingual mucosa in irradiated albino rats.Methods: 30 adult male albino rats were used. Rats were then divided randomly into three groups, 10 rats each. Group A: Irradiated rats received no treatment. Group B: Irradiated rats received a single dose of 1×106 bone marrow derived mesenchymal stem cells (BM-MSCs) injected intravenously, immediately after irradiation. Group C: Irradiated rats received a single dose of 30 μg microvesicles (derived from bone marrow stem cells) injected intravenously, immediately after irradiation. Each animal group was further divided into two subgroups (5 rats each) according to their date of scarification as follows: Subgroup I: The animals were sacrificed at day 3 following irradiation. Subgroup II: The animals were sacrificed at day 7 following irradiation. After scarification the tongue was dissected and examined histologically, histochemically and by real time-polymerase chain reaction (RT-PCR) for keratin 10 expression.Results: Histologically, irradiated groups received no treatment (groups AI&AII) showed marked epithelial and connective tissue degeneration. On the other hand both treated irradiated groups showed marked improved histological features (group B & C) during both experimental periods (3 and 7 days). However the microvesicles treated group demonstrated normal histology especially at 7 days. Histochemical examination using masson´s trichrome stain revealed an increased collagen density in groups (B & C) as compared to group A during both experimental periods. This increase was statistically significant. RT-PCR results showed statistically significant increase in keratin 10 expression in groups (B & C) as compared to group A during experimental periods I & II. Conclusions: Both BM-MSCs and microvesicles showed healing power in atrophied epithelium and connective tissue of dorsal lingual mucosa after radiotherapy, however treatment with microvesicles revealed more positive effect than BM-MSCs.
Radiotherapy
Bone marrow derived stem cells
Microvesicles
Keratin 10
2018
04
01
1377
1388
https://edj.journals.ekb.eg/article_77395_86100940817e871fa6d64c4717b2d775.pdf