The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401CLINICAL AND RADIOGRAPHIC EVALUATION OF CORTICO-CANCELLOUS BONE MIX XENOGRAFT (OSTEOBIOL GEN-OS®) IN THE TREATMENT OF HUMAN PERIODONTAL INTRABONY DEFECTS140914187453910.21608/edj.2017.74539ENAlaa MoustafaAttiaAssociate Professor Oral Medicine and Periodontology, Faculty of Dentistry, Al-Azhar University (Assuit Branch)
and Umm Alqura UniversityJournal Article20200229Background and objective: Bone substitutes are widely used to promote bone formation and periodontal regeneration. The aim of this study was to compare the clinical and radiographic effectiveness of cortico-cancellous bone mix xenograft (Osteobiol Gen-Os) in the treatment of intrabony defects. <br />Materials and methods: Twenty systemically healthy chronic periodontitis patients, radiographic observation showed vertical intrabony defect of ≥ 5 mm after non-surgical periodontal therapy, subjects were categorized into group 1 (G1) treated by xenogrfat and group 2 (G2) treated by open flap debridement only. At baseline, 6 and 12 month after surgery the patients were evaluated clinically by: plaque index (PI), gingival index (GI), pocket depth (PD), clinical attachment loss (CAL), and radiographically to measure the bone density and reduction of intrabony defect depth. <br />Results: Significantly clinical improvements of PI and GI were observed in both groups. In G1, The mean values of PD and CAL were representing highly significant differences between the three time intervals (baseline, 6 and 12 month, (P ≤ 0.01). In G2, the statistical comparisons between the mean values of PD at 3 time intervals, also CAL mean values at 6 month versus baseline were highly significant (P ≤ 0.01). Radiographically, the bone density and reduction of defect depth were improved in G1 compared to G2. The mean values of bone density and reduction of defect depth were high significantly increased in G1 versus G2 at 6 and 12 month (P ≤ 0.01). <br />Conclusions: The results of the current study indicated that cortico-cancellous xenograft (Osteobiol Gen-Os) significantly improved of clinical and radiographic outcomes over the course of the study.https://edj.journals.ekb.eg/article_74539_5124c91f3b901441864b4df9b5088cad.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401EVALUATION OF DIFFERENTIAL MONOCYTES IN THE DMBA/HCPE CARCINOGENESIS MODEL UNDER THE INFLUENCE OF SOME PHYTOCHEMICAL AGENTS141914357454010.21608/edj.2017.74540ENMagda M. AlyHassanProfessor Department of Oral Pathology, Faculty of Dentistry, Suez Canal University, EgyptGhada A.Abd El AtifLecturer Department of Oral Pathology, Faculty of Dentistry, Suez Canal University, EgyptJournal Article20200229Dimethylbenza-a-anthracene (DMBA), is a potent carcinogen that has an immunosuppressive effect on hamsters. Nigella sativa whole oil (NSO) and thymoquinone (TQ), are phytochemicals that have an antioxidant and protective action on the hamster cheek pouch epithelium (HCPE/DMBA) carcinogenesis model. <br />The aim is to find which of the two ingredients TQ & NSO has more immune-enhancing activity comparable with its protective and/or chemotherapeutic activity against the carcinogenic effect of DMBA on HCPE, through evaluation of different peripheral monocytes. <br />Material &methods: NS whole oil (NSO), and thymoquinone (TQ) were given before-, with- or after DMBA painting the hamster’s left cheek pouch. Before each animal’s sacrificing, two ml of blood was drawn in a fine heparinized capillary tube to estimate the total WBCs, total lymphocytes, MID cells, and granulocyte’s count by an automatic count system (Cell-DYN1700).<br />Blood results were statistically analyzed using One-way ANOVA test between groups for each phase followed by LSD (Least Square Difference) test for multiple comparisons between different phases. All pouches were surgically-excised and examined microscopically to compare the histopathologic changes of DMBA-painted pouches, NSO-and TQ-treated groups, with untreated pouches as well as pouches of the untreated group.<br />Histopathologic results: the groups given each of the tested ingredients for 2 wks showed normal appearing thin epithelium, with slight hyper-keratinization as compared with untreated pouches. Severe epithelial dysplasia was seen after 6 wks of DMBA, but when NSO or TQ was given for 2wks followed by DMBA for 6wks, mild dysplasia was seen i.e. both have protective effect. When DMBA for 6wks was followed by NSO or TQ for 6wks mild dysplasia was recorded .i.e. both had a therapeutic effect through regressing severe dysplasia to mild dysplasia in 6 weeks. <br />Mononuclear cells results: DMBA for 6wks resulted in statistically significant reduction in total WBCs &lymphocyte counts than control group, as well as when DMBA for 6wks was followedhttps://edj.journals.ekb.eg/article_74540_3fa98381d0e4e654b0f7acb07a99938c.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401THE IMPACT OF OMEGA-3 FATTY ACIDS COMBINED WITH INITIAL PERIODONTAL THERAPY ON SALIVARY VISFATIN AND TNF-Α LEVELS IN CHRONIC PERIODONTITIS PATIENTS143714477454110.21608/edj.2017.74541ENHesham M.El-SharkawyAssociate Professor of Oral Medicine and Periodontology, Faculty of Dentistry, Mansoura University.Samah H.ElmeadawyAssociate Professor of Oral Medicine and Periodontology, Faculty of Dentistry, Mansoura University.Journal Article20200229Recent investigations have demonstrated the positive therapeutic effects of omega-3 polyunsaturated fatty acids (PUFAs) on several chronic inflammatory diseases such as rheumatoid arthritis, chronic inflammatory bowel diseases and periodontitis. The objective of the present study was to evaluate the impact of omega-3 PUFAs in conjunction with initial periodontal therapy (IPT) on periodontal clinical parameters and salivary markers in patients with chronic periodontitis. Thirty four systemically healthy individuals with advanced chronic periodontitis were enrolled and randomly assigned into two groups. The control group (IPT, n=17) was treated with IPT alone whereas the test group (IPT+omega-3, n=17) was treated with IPT and daily dietary supplementation of omega-3 PUFAs (in the form of 2 grams of fish oil capsules per day) for three months. Clinical parameters were recorded at baseline and 3 months following therapy for both groups. Saliva samples were collected at the same time points and analyzed for visfatin and tumor necrosis factor-α (TNF-α). After 3 months, clinical periodontal parameters of advanced chronic periodontitis were significantly improved in both groups. The omega-3 group showed significant greater pocket depth (PD) reduction and clinical attachment (CAL) gain compared to the control group after therapy. Salivary TNF-α levels showed a statistically significant decrease in the omega-3 group at 3 months compared to the control group. Salivary visfatin levels were reduced significantly at 3 months in both groups without any significant variation. It was concluded that dietary supplementation with omega-3 PUFAs could be a potential viable adjunct to IPT that significantly improves all periodontal parameters and reduces salivary visfatin and TNF-α levels in advanced chronic periodontitis. Interestingly, salivary visfatin could be a useful periodontal biomarker to monitor responses to periodontal therapy.https://edj.journals.ekb.eg/article_74541_f4e24d8e2b91695a6006b0f7472d045e.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401A CORRELATION BETWEEN FASTING BLOOD GLUCOSE AND PERIODONTAL FINDING IN DIABETIC PATIENTS HAVING CHRONIC PERIODONTITIS AND TREATED WITH (TRAUMEEL) DRUG144914577454210.21608/edj.2017.74542ENMaha R.TaalabLecturer of Periodontology, Oral Medicine, Diagnosis and Radiology, Faculty of Dentistry, Alexandria UniversityJournal Article20200229Objective to investigate the influence of Traumeel drug as adjunctive to non surgical periodontal intervention in patients with poorly controlled type 2 diabetes associated with chronic periodontitis on both periodontal parameters and metabolic findings, and to correlate the levels of fasting blood glucose (FBG) , and fasting blood insulin (FBI) to the clinical periodontal parameters in those patients after treatment.<br />Methods and Patients A total of 20 patients having moderately poorly controlled (HbA1c between 8%-10%) type 2 diabetes mellitus associated with moderate to severe chronic periodontitis were selected and randomly divided into two groups according to whether they underwent periodontal intervention alone (control group) or periodontal intervention adjunctive to traumeel tablets with a dose of of 1 tablet 3 times daily for 1 week. The levels of serum tumor necrosis factor á (TNF-α), FBG,FBI and glycosylated hemoglobin(HbC1) were measured at baseline and after 3 months. The periodontal parameters including Probing pocket depth(PPD), Clinical attachment loss (CAL) and Modified Gingival index (MGI) were also measured at baseline and after 3 months<br />Results The levels of both metabolic parameters and clinical periodontal parameters were improved significantly in both test and control groups after 3 months but on comparing percent of change D at p ≤0.05between control and tablet groups after 3 months ,test group showed more reduction in all parameters except Hbc1compared to control group .There were also a statistically significant correlation between periodontal parameters and FBG and FBI in the test group only.<br />Conclusion The adjunctive use of homeopathic Traumeel tablets with periodontal intervention offered better results concerning periodontal and metabolic state .https://edj.journals.ekb.eg/article_74542_f1296ad48680588c7051f2f06efa86b1.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401MYOEPITHELIAL CELL AND ITS PROSPECTIVE ROLE IN SOME SALIVARY GLAND TUMORS (AN IMMUNOHISTOCHEMICAL STUDY)145914667454310.21608/edj.2017.74543ENAshraf IbrahimGaafarAssociate Professor of Oral and Dental Pathology, Faculty of Dental Medicine, Al-Azhar University (Assiut Branch, Egypt).Hesham AhmedDameerAssociate Professor of Oral Biology, Faculty of Dental Medicine (boys), Al-Azhar University (Cairo, Egypt).Mohamed GomaaKhalilLecturer of Oral and Dental Pathology, Faculty of Dental Medicine Al-Azhar University (Assiut Branch, Egypt).Journal Article20200229Salivary gland tumors are characterized by a wide variety of histological types which makes their classification and diagnosis difficult. It should be noted that the diversity in the occurrence and dilemma regarding the pathogenesis of salivary gland tumors is due to lack in distinguishing the cells participating in its oncogenesis, especially the Myoepithelial cells (MECs). MECs are normal constituent of the major and minor salivary glands, they are found between the basal lamina and the acinar or ductal cells. Proper and extensive studies regarding MECs are varied and thus have posed difficulty for a pathologist to understand this cell. Numerous functions of MECs have been described, the most important of them being important for contraction of the glands, also, it has been found to prevent tumor progression. In this study we try to make a thorough description of this cell and its role in some salivary gland tumors. 24 formalin-fixed, paraffin-embedded specimens of salivary gland tumors (7 cases of pleomorphic adenoma, 6 cases of mucoepidermoid carcinoma, <br />6 cases of Adenoid cystic carcinoma, and 5 cases of clear cell carcinoma) were used in this study. <br />The specimens were cut at 5μm thick for H&E staining as a routine stain and calponin immunostaining. Calponin is a 34 kDa protein, it is a family of actin filament-associated proteins and it is a sensitive immunohistochemical marker for MECs. The present study revealed positive staining reaction for calponin in pleomorphic adenoma and adenoid cystic carcinoma and negative calponin reaction in mucoepidermoid carcinoma and clear cell carcinoma. These findings demonstrate that myoepithelial cells play a role in the pathogenesis of some salivary gland neoplasms and no or minimal role in others.https://edj.journals.ekb.eg/article_74543_e685a8a64c3e0dfe9a46e358da01d484.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401CLINICAL AND RADIOGRAPHIC EVALUATION OF DEMINERALIZED FREEZE-DRIED BONE ALLOGRAFT PLUS HYALOURONIC ACID VERSUS DEMINERALIZED FREEZE-DRIED BONE ALLOGRAFT PLUS PLATELET-RICH PLASMA FOR THE TREATMENT OF PERIODONTAL INTRABONY DEFECTS – A RANDOMIZED BLIND STUDY146714757472610.21608/edj.2017.74726ENNaglaa M.El-WakeelAssociate professor of Oral Medicine, Diagnosis, Periodontology and Radiology, Faculty of Dentistry, Al- Azhar
(Girls Branch) and MSA Universities , Egypt.Journal Article20200301Objectives: The present study aimed to evaluate the effect of ; 1- combined demineralized freez dried bone allograft( DFDBA)plus hyaluronic acid(HA) compared to DFDBA alone, and 2) combined DFDBA/HA compared to DFDBA /PRP in treatment of periodontal infrabony defects(PIDs). <br />Subjects and methods:45 infrabony defects in subjects with chronic periodontitis were randomly divided into 3 groups treated with, group 1: DFDBA/HA, group 2: DFDBA/PRP and group 3: DFDBA alone. Clinical and radiographic measurements were made at baseline and 12 months after surgery. ANOVA followed by Tukey’s post hoc test were used to analyze the data. <br />Results: A statistically significant improvement in clinical attachment level( Cal) and pocket depth ( PD) as well as radiographic bone fill were reported in group 1 and 2 compared to DFDBA alone( p ≤ 0.05), a non significant difference was reported between group 1 and group 2 regarding clinical and radiographic parameters(p > 0.05).<br />Conclusion : Results of the present investigation suggests that the combination of DFDBA/HA is more effective both clinically and radiographically than DFDBA alone in treatment of PIDs and that both HA and PRP combined with DFDBA are equally effective in treatment of these defects.https://edj.journals.ekb.eg/article_74726_c0e3751ff901681b601bf1cd57195f71.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401COMPARING THE EFFECT OF SYSTEMP ON THE ULTRA STRUCTURE AND PERMEABILITY OF ENAMEL FOLLOWING TWO DIFFERENT TECHNIQUES OF ENAMEL ETCHING AN IN VITRO STUDY147714867454410.21608/edj.2017.74544ENZeinab A.SalemLecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University.Aboushady,I.M.Lecturer, Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University.Journal Article20200229Background: Acid etching results in an unintentional demineralization of the enamel surface, while laser conditioning might provide microspaces which then remineralize by trapping free ions; thus enhancing resistance to caries. Since, Systemp desensitizer was very successful in reducing the incidence of post-operative pain; consequently, this in-vitro study was designed to evaluate the effect of Systemp on the surface micro roughness and permeability of enamel following two different techniques of enamel etching; acid and laser etching; in premolar teeth.<br />Methodology: Fifty freshly extracted non-carious premolar teeth were used. The teeth were divided into 5 groups each containing 10 teeth. Group I: control group, group II: teeth exposed to phosphoric acid etching. Group III: teeth exposed to low level laser (LLL) etching. Group IV: Systemp applied to acid etched enamel. Group V: Systemp applied to teeth etched by LLL. The extent of dye penetration was measured using stereo-microscopy and the obtained data were statistically analyzed. The surfaces of the specimens were examined using scanning electron microscopy. <br />Results: scanning electron microscopic (SEM) examination of group II revealed an obviously porous enamel surface with type III enamel etching pattern. On the other hand, following laser etching, enamel surface showed areas of non removed prismless enamel while most enamel presented type I enamel etching pattern. Systemp application rendered the surface more homogenous especially in the acid etched group. Statistical analysis revealed that the distance travelled by the dye was significantly greater in group II (acid etched group) (mean±SD=1,406.06±0.721), than group III (laser etched group) (mean±SD=1,235.35±0.771). However, group IV (Systemp applied to acid etched enamel) (mean±SD=1,078.47±0.634) showed significant reduction than group V (Systemp applied to LLL etched enaamel) (mean±SD=1,198.44±0.583) as the p-value was less than 0.05. <br />Conclusion and recommendations: despite laser advantages, laser-etching applications should be improved. Besides, desensitizing agents, like Systemp, could be efficient in sealing etched enamel surface, which could reduce sensitivity resulting from leakage through enamel into the underlying dentin.https://edj.journals.ekb.eg/article_74544_7ef4d0e53ce8a75bd7e0807a55d0cb24.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401EVALUATION OF THE EFFECTS OF LACTOBACILLI CONTAINING PROBIOTICS IN NON-SURGICAL TREATMENT OF CHRONIC PERIODONTITIS: A CLINICAL AND MICROBIOLOGICAL STUDY148714947454510.21608/edj.2017.74545ENReham LotfyAggourLecturer of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology, Faculty of Dentistry, October 6 UniversityJournal Article20200229Background & Objectives: There is limited information on the effects of beneficial bacteria (probiotics) on management of periodontal disease. The purpose of the present study was to investigate the clinical and microbiological effects of Lactobacilli containing probiotic sachets as an adjunct to non-surgical periodontal therapy.<br />Methods: Thirty chronic periodontitis patients, who satisfied the inclusion and exclusion criteria, were randomly assigned to: (1) scaling and root planing (SRP) (control group, 15 patients) or (2) SRP plus Lactobacilli containing sachets (test group, 15 patients). Full mouth SRP was performed on day 0. On the same day, Lactobacilli containing sachets were given to the patients to be taken for 1 month. Periodontal clinical parameters and the proportion of black pigmented anaerobic rods (BPARs) and Lactobacilli levels were recorded on day 0, at 1 month and at 6 months post therapy. <br />Results: Both treatment modalities resulted in a statistically significant improvement in clinical parameters (p<0.05) after 1 and 6 months. No intergroup statistically significant differences were observed (p>0.05). Microbiological analysis showed a statistically significant reduction of BPARs proportion for both groups, at 1 and 6 months, when compared with baseline values (p<0.01). A statistically significant intergroup difference in proportion of BPARs were found at 1 month in favor of the probiotic group (p<0.05), however, the intergroup difference was not significant at 6 months evaluation period. A statistically significant negative correlation between clinical parameters (FMBI: p =0.032, PPD: p =0.040) and Lactobacilli count was observed. This inverse correlation was also seen with subgingival colonization of BPARs (p = 0.018).<br />Conclusion: Both treatment modalities provided comparable clinical results, however microbiological changes were more evident in the probiotic group. Oral probiotics can repopulate the beneficial microflora and reduce the pathogenic bacteria, however, repeated application is required.https://edj.journals.ekb.eg/article_74545_cb092f6f0176f76a34e950b1721f6c4d.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401EFFECT OF PLATELET-RICH FIBRIN PALATAL BANDAGE ON PAIN SCORES AND WOUND HEALING AFTER FREE GINGIVAL GRAFT: A RANDOMIZED CONTROLLED CLINICAL TRIAL149515067454610.21608/edj.2017.74546ENMaha A.BahammamAssociate professor of periodontology. Faculty of Dentistry-King AbdulAziz University, Jeddah, SAEman Y.ElFirtAssociate professor of periodontology. Faculty of Dentistry-King AbdulAziz University, Jeddah, SA. and Cairo
University, Cairo,Egypt.Journal Article20200229Background: This prospective randomized clinical trial was conducted to determine whether the application of a platelet-rich fibrin (PRF) palatal bandage after harvesting free gingival grafts (FGG) would improve healing of donor sites and decrease pain scores and patient discomfort. <br />Methods: Twenty-four patients received FGG to augment keratinized tissue dimensions. The application of a PRF bandage was decided randomly (n = 12 in each group). Patients reported their pain levels for the first 7 days of healing using the visual analog scale (VAS), 101-point numerical rating scale (NRS-101), and 4-point verbal rating scale (VRS-4). The patients’ pre-operative anxiety levels were measured using three anxiety scales. The healing of the donor sites was evaluated and compared to preoperative records. Data were assessed and recorded before surgery and at 1, 2, 3, 4, and 8 weeks postoperatively. <br />Results: Dental anxiety, state-trait anxiety were evaluated at base line and correlated to the postoperative pain scores. There were no significant differences in anxiety levels between the two groups, yet they could potentially influence the outcome of any surgery and were included as covariates. The patients in the PRF group reported significantly lower pain scores and their pain levels returned to baseline levels earlier, compared to the control group. They were also assessed as having better wound healing over a 2-month follow-up period, based on color, contour, and texture indices. <br />Conclusion: PRF palatal bandages significantly reduced postoperative pain and discomfort, and facilitated wound healing after harvesting FGG.https://edj.journals.ekb.eg/article_74546_cdb63ff57e820ce094bf36e64bcaf887.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401EVALUATION OF THE SALIVARY LEVEL OF MIR-21 IN CIGARETTE SMOKERS: CASE-CONTROL STUDY150715127454710.21608/edj.2017.74547ENShereenAliLecturer, Department of Oral Medicine and Periodontology, Faculty of Oral and Dental Medicine, Cairo UniversityEmanAmerAssociate Professor, Department of Medical Biochemistry, Faculty of Pharmacy, Ahram Canadian University.Journal Article20200229Background: Salivary biomarkers are attractive non-invasive tools for screening oral cancer especially in high risk individuals such as smokers. The present study observed the salivary level of microRNA-21 to verify their value as diagnostic marker for smokers.<br />Methods: The study sample comprised 40 males: 20 non-smokers and 20 smokers. Whole unstimulated saliva samples were collected. The resulting data was analyzed for any statistical significance.<br />Results: Smokers had statistically significant higher salivary microRNA-21 level than non-smokers.<br />Conclusions: Our study demonstrated elevated levels of salivary microRNA-21 in cigarette smokers. Salivary miR-21 could be a promising new diagnostic marker for cigarette smokers. <br />We recommend conducting further studies to investigate its diagnostic value as a marker for oral cancer susceptibility in cigarette smokers.https://edj.journals.ekb.eg/article_74547_9c3bb57b21533f31bb339c69722a5021.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401CONE BEAM COMPUTED TOMOGRAPHIC ASSESSMENT OF MAXILLARY SINUS VOLUME VARIABILITY AMONG ADULT EGYPTIANS151315217454810.21608/edj.2017.74548ENGihan Gamal El DinEl DesoukyAssistant Professor of Oral Radiology, Faculty of Dentistry, Suez Canal University.Ahmad MohamadEl RawdyLecturer of Oral Radiology, Faculty of Dentistry, Suez Canal University.Journal Article20200229<br /> Anatomical variability in the maxillary sinus volume has been studied and reported by several researchers. <br />Objective: The purpose of the present study was to assess the variety in the maxillary sinus volume between genders and between the right and left sides using cone beam computed volumetric imaging. <br />Methods: The present retrospective study was carried on fifty cone beam computed tomography (CBCT) records of adult Egyptians aging from 20-40 years. The records were equally partitioned into two groups; the male group (G1) and the female group (G2). Volumetric examination of the antrum was conducted and statistically analyzed to compare between right and left sides and between males and females.<br />Results: Statistically significant differences was found in the antral volume between male and female subjects as well as between the right and left sides. <br />Conclusion: The volume of the maxillary sinus (MS) in male subjects was larger than the female subjects, in addition, the right-side volume was found to be larger than the left side for both genders.https://edj.journals.ekb.eg/article_74548_6dbb4de7c24662304764b94c3c7296f2.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401IMPACT OF ORAL CONTRACEPTIVES ON THE PERIODONTIUM: A HISTOLOGICAL STUDY152315297454910.21608/edj.2017.74549ENHeba A.ShawkyLecturer of Oral Medicine and Periodontology, Oral Medicine and Periodontics Department, Faculty of Dentistry,
Pharos University in Alexandria, Egypt.Journal Article20200229Background: Sex hormones play an important role in periodontal tissue integration. Oral hormonal contraceptive pills (OCPs) were developed over 50 years ago. Different studies reported higher progression rate of gingival inflammation in females using OCPs. The present study was performed to evaluate histologically the influence of oral contraceptives on periodontal tissues.<br />Materials and Methods: A total of thirty healthy female Wistar albino rats aging between 10 to 12 weeks and weighing 230 – 260 gm were enrolled in this study. The animals were divided into two groups, fifteen rats each. Group I (Control group), rats receiving no medication. Group II (Study group), rats received Microcept® monophasic combined oral contraceptive tablets for 36 days. Histological examination of the peridontium was accomplished using light microscopy. <br />Results: Light microscopic examination of the control group revealed that the gingival epithelium was parakeratinized stratified squamous epithelium with long pointed rete pegs. Pyknotic nuclei were seen in the keratinous layer. Concerning the study group, the gingival epithelium revealed orthokeratinzed stratified squamous epithelium with short and blunt rete pegs and absence of nuclei in keratinous layer. Dentin and cementum showed external resorption.<br />Conclusion: The current study showed that oral contraceptives exhibited histological changes on the periodontium which can be correlated to different clinical findings.https://edj.journals.ekb.eg/article_74549_7214953bd61db2a73ae5645e4061ce60.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401IN VITRO EVALUATION OF MECHANICAL AND STRUCTURAL PROPERTIES OF LEUKOCYTE-PLATELET-RICH FIBRIN AND PLATELET-RICH FIBRIN MATRIX153115397455010.21608/edj.2017.74550ENReham LotfyAggourLecturer of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology, Faculty of Dentistry, October 6 UniversityHadeel A.SabryAssociate professor of Dental Biomaterials, Faculty of Dentistry, October 6 UniversityGihan ShehatahHassanLecturer of Oral Biology, Faculty of Dentistry, Tanta UniversityJournal Article20200229Objectives: The aim of this study was to investigate the mechanical properties as well as the structural analysis of autologous platelet-rich fibrin matrix (PRFM) as compared to autologous leukocyte-platelet-rich fibrin (L-PRF). <br />Materials& Methods: Sixty three cubic centimeters (cc) venous blood was obtained from 10 male volunteers. Twenty seven cc were used to prepare 3 specimens of L-PRF (group 1), twenty seven cc were used to get 3 specimens of PRFM (group 2) and nine cc for whole blood analysis. After centrifugation, blood analyses were performed on the residual plasma after collecting L-PRF and PRFM. The L-PRF and PRFM membranes were processed for examination by light microscopy and transmission electron microscopy (TEM) and their mechanical properties were measured by a universal testing machine. <br />Results:Tensile strength and maximum tensile strain of L-PRF group was significantly higher than PRFM group (p < 0.01). PRFM group was significantly stiffer than L-PRF group (p < 0.01). Light microscopy revealed that the platelets were less but more equally distributed in the PRFM than L-PRF. The border between the cellular components and the fibrin network appeared thicker in the PRFM samples than in the L-PRF samples and shows a highly organized network with continuous integrity. TEM showed that both membranes contained two components: a fibrillar material similar to fibrin filaments, and a cellular component that contains human platelet cells. TEM analysis demonstrated that PRFM membrane had more nonactivated platelets.<br />Conclusion: The present study shows that the structural and mechanical properties of PRFM may fit characteristics desirable for GTR procedures more than that of L-PRF.https://edj.journals.ekb.eg/article_74550_4d3ad71fdc536951deb9e04394289cfd.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401EVALUATION OF SALIVARY AND SERUM VISFATIN IN ORAL BULLOUS EROSIVE LICHEN PLANUS AND ORAL SQUAMOUS CELL CARCINOMA154115497455110.21608/edj.2017.74551ENWesamAbdel MoneimAsso.Professor at Department of Oral Medicine, Periodontology and Diagnosis, Faculty of Oral and Dental Medicine, Cairo University.AmiraMagedLecturer at Department of Oral Medicine, Periodontology and Diagnosis, Faculty of Oral and Dental Medicine, Cairo University.OlfatShakerProf. at Department of Medical Biochemistry, Faculty of Medicine, Cairo UniversityJournal Article20200229Introduction: Bullous Erosive Oral Lichen Planus (BEOLP) is a potentially malignant condition with high rate of transformation into Oral Squamous Cell Carcinoma (OSCC), and aiming to reduce the morbidity and mortality rate arising from OLP; the early diagnosis of oral cancer was recommended. Many biomarkers were found to be released into the serum and the saliva during the course of both diseases, BEOLP and OSCC. One of these proteins is visfatin.<br />Aim of the study: The aim of this study was to evaluate salivary level of visfatin and to find out if it can be used instead of its serum level as an early diagnostic biomarker for patients with either BEOLP or OSCC.<br />Subjects and Methods: The study was conducted on 3 groups; group (A) which included fifteen medically free subjects, group (B) which included fifteen patients suffering from BEOLP, and finally group (C) which included fifteen patients suffering from OSCC. Serum and salivary samples were collected and visfatin levels were measured using an ELISA kit (Enzyme Linked Immuno Sorbent Assay). Data were analyzed by SPSS. <br />Results: The present study detected no statistically significant difference between mean visfatin levels in BEOLP and OSCC groups; both showed statistically significantly higher mean levels than control group which showed the lowest mean visfatin level. It Also revealed a statistically significant positive (direct) correlation between serum and salivary visfatin levels (r = 0.835, P-value <0.001) i.e. an increase in serum level of visfatin is associated with an increase in salivary level of Visfatin.<br />Conclusion: Salivary level of visfatin can be used instead of its serum level as a biomarker for BEOLP and OSCC.https://edj.journals.ekb.eg/article_74551_4dfb9a9f53ba64043d9a96df0f731351.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401EVALUATION OF BCL-2 EXPRESSION IN CIGARETTE SMOKERS155115587455210.21608/edj.2017.74552ENShereenAliLecturer, Department of Oral Medicine and Periodontology, Faculty of Dentistry, Cairo University, Lecturer,
Department of Oral Medicine, Periodontology and Oral Radiology, Faculty of Dentistry, Misr International
University (MIU).Journal Article20200229Background: Tobacco use is the most important risk factor linked to oral cancer. Tobacco influences the DNA repair, cell cycle control, proliferation and apoptosis. The present study observed bcl-2 expression to verify its value as prognostic markers in cigarette smokers.<br />Methods: The study sample comprised 40 smoking males; 20 with hyperplasia and 20 with dysplasia. The duration of smoking and the number of cigarettes were recorded. Hematoxylene and Eosin stained sections were used for histopathologic examination and immunostained sections for evaluation of the expression of bcl-2. The resulting data were analyzed statistically.<br />Results: The dysplasia group had a higher mean duration of smoking, number of cigarettes per day and total number of cigarettes as well as higher mean area % and stronger bcl-2 immunoreactions compared to that of the hyperplasia group. <br />Conclusions: Bcl-2 may be a valuable prognostic marker in evaluating the malignant potential of hyperplastic and dysplastic lesions in cigarette smokers.https://edj.journals.ekb.eg/article_74552_40ed94baae69a47851f8ebd3db7652fb.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401POSSIBLE SYNERGISTIC ANTI-CARCINOGENIC EFFECT OF MORINGA OLEIFERA AND CISPLATIN ON HUMAN SQUAMOUS CELL CARCINOMA155915797455310.21608/edj.2017.74553ENDina M.Abdel KhalikLecturer of Oral Biology, Faculty of Dentistry, Ain Shams UniversityHala A.El-kammarLecturer of Oral Pathology, Faculty of Dentistry, Future UniversityJournal Article20200229Introduction Moringa Oleifera (MO) is a cheap source of glucosinolates, falvonoids, among other components that may be used in the treatment of various illnesses including cancer. Cisplatin is a widely used anticancer chemotherapeutic agent. More than 90% of all oral cancers are squamous cell carcinoma (SCC). In spite of the advances in treatment modalities the survival rates have not improved.<br />Aim of the work: The aim of the present work was to assess the anticarcinogenic potentiality of MO extract alone and when used in conjunction with different doses of Cisplatin in treatment of human squamous cell carcinoma cell line.<br />Materials and methods: MO leaf extract, Cisplatin® 10mg / 10 ml 1 vial and HEp2 cells were used in the present study. In the present study, Cisplatin 20 mg, 50 mg and MO extract were used independently and in combination on HEp2 cell line. The expressions of caspase 9 and Beta catenin, and viability were assessed after 24 and 48 hours. <br />Results: Phase contrast microscopic results showed variations in morphology and number of cells between different subgroups. Highest Caspase 9 gene, least Beta catenin and least viability were noted when HEp2 cells were treated with Cisplatin 50 mg and MO for 48 hours.<br />Conclusions: MO has an effective anticarcinogenic role on HEp2 cell line. The combined use of MO and Cisplatin is more effective than either of them alone, in a dose and duration dependant manner.https://edj.journals.ekb.eg/article_74553_d770456d12f6155da4543dfa9397ed6a.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401ASSOCIATION BETWEEN ANKYLOSING SPONDYLITIS AND CHRONIC PERIODONTITIS IN DUHOK: A CASE-CONTROL STUDY158115897455410.21608/edj.2017.74554ENSaeed AliMohammedDepartment of Periodontics, College of Dentistry, University of Duhok, Duhok, Kurdistan region, Iraq.Journal Article20200229Background and objectives: Ankylosing spondylitis (AS) and periodontal disease (PD) are characterized by dysregulation of the host inflammatory response, resulting in soft and hard connective tissue destruction. AS has been related toother inflammatory diseases, however, there is a paucity of data on whether AS is associated with inflammatory PD. The aim of this study to investigate the possible association between AS and chronic periodontitis.<br />Methods: The case-control study included 90 individuals,45 documented patients with AS and had been diagnosed by rheumatologists in the specialized center of rheumatic disease and medical rehabilitation and referred to the department of periodontics, College of Dentistry, University of Duhok and another 45 clinically healthy individuals with PD as a control group. Among studied individuals, 65 were males and 25 were female. A detailed periodontal examination was performed in all subjects, included probing pocket depth, epithelial insertion level (clinical attachment loss) and plaque index and a form of questionnaires was filled from the patients included gender, age, duration of AS disease, medication, risk factors of PD like smoking and level of education.<br />Results : No significant differences were found in two studied groups based on age groups, level of education, smoking, duration of disease and the type of used drugs, but a significant difference was found between two groups based on gender in which AS was more common among males than females.<br />Conclusions: These results suggest that an association may exist between AS and periodontal disease. Such an association would be important since knowledge of an effect of periodontal treatment on AS disease activity or vice versa would lead to the need for close collaboration between rheumatologists and dentists when treating these diseaseshttps://edj.journals.ekb.eg/article_74554_ddf645f6789c9e49d9e259f96436468d.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948463Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20170401ASSESSMENT OF GINGIVAL AND TOTAL AN TI-OXIDANT CAPACITY IN SMOKERS AND NONSMOKER’S CHRONIC PERIODONTITIS PATIENTS FOLLOWING NON-SURGICAL PERIODONTAL THERAPY159115997455510.21608/edj.2017.74555ENRania FaroukAbdulmaguidLecturer at Oral Medicine and Periodontology Department Faculty of Dentistry MSA UniversityHakem H.ElsayedHead of Oral Medicine & Periodontology Dept. Faculty of Dentistry. MSA UniversityJournal Article20200229Periodontal disease is a chronic infectious disease caused by specific bacteria and associated of inflammation of the supporting structures of teeth, there is a strong evidence for oxidative stress implication in the progression of periodontitis. Oxidative stress is a condition arising when there is a serious imbalance between the levels of free radicals in a cell and its antioxidant defenses which leads to tissue damage when antioxidant systems are unable to counteract the free radicals action efficiently Smoking also increases ROS production and is a significant source of oxidative stress. It causes depletion of systemic endogenous antioxidant capacity, resulting in increased pro-oxidant burden. Several recent studies have indicated that total antioxidant capacity (TAOC) of plasma seems to be compromised in chronic periodontitis (ChP). The aim of this study is to investigate serum and gingival crevicular fluid GCF (TAOC) in nonsmoker and nonsmoker patients with ChP to assess the effects of non-surgical periodontal therapy on the levels of (TAOC).<br />Methods: 20 patients are selected from the outpatient clinic in faculty of dentistry MSA university, 10 patients with severe periodontitis and were nonsmokers (group 1) while the other 10 also with severe periodontitis but were heavy smokers (group 2), both groups received non-surgical periodontal therapy, and daily oral hygiene instructions. Serum (TAOC), GCF samples and periodontal parameters (Plaque index PI, Gingival index GI, Probing depth PD and clinical attachment loss CAL are assessed at base line and one month following therapy.<br />Results All measures were recorded at base line and after 1 month follow up. Serum TAOC levels showed a significant increase in the chronic periodontitis group (group 1) following the non-surgical periodontal therapy with P value of 0.0146, also in the chronic periodontitis smoker group (group 2) the serum TAOC levels showed a highly significant increase following the non-surgical periodontal therapy with P value of 0.0025, with highly significant difference between both groups where the P value was For the Gingival index, GI, there was a highly significant difference in group 1 and group 2 at the base line and following the treatment with P value <0.0001, with a significant difference between both groups with P value of 0.0368 For the plaque index, PI there was a highly significance difference in group 1 with P value Conclusion In both chronic periodontitis smoker’s and nonsmoker’s patients, the TAOC levels in serum and gingival fluid are significantly low. Non-surgical periodontal treatment seems to reduce oxidative stress in smokers and non-smokers chronic periodontitis patients, the chronic periodontitis smokers group showed a Significant reduced TAOC in both serum and gingival fluids than the chronic periodontitis non-smokers group.https://edj.journals.ekb.eg/article_74555_479dc1eb8d669aac46bbc8a5c71e25df.pdf