The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Evaluation Of Curcumin And Chitosan Protective Effect On Submandibular Salivary Gland Toxicity Induced By Monosodium Glutamate In Male Albino Rats1187119715975210.21608/edj.2021.55176.1426ENAsmaaElgamalLecturer, Oral Biology Department, Faculty of Dentistry, Beni-Suef University, Egypt.0000-0001-6438-4793Journal Article20201225ABSTRACT<br /> Monosodium glutamate is a well-known food flavor additives that is widely used all over the world but its chronic intake leads to many reported side effects. <br /> Aim of the study: The goal of this study was to evaluate the prophylactic role of chitosan and curcumine extract, as a well-known antioxidant, against the submandibular salivary gland (SMG) damage induced by chronic intake of monosodium glutamate (MSG).<br /> Material and methods: 36 adult male Albino rats were randomly divided into 4 groups; Group 1 (control) group, group2 (MSG group): rats received a daily dose of MSG only by oral gavage, group 3 ( MSG + Chitosan treated group): rats received a daily dose of MSG + chitosan and finally group 4 ( MSG + curcumin treated group): rats received a daily dose of MSG + curcumin. After 6 weeks, the submandibular salivary glands were excised and processed for histological and caspase 3 immunohistochemical examination. <br /> Results: histological examination by H&E stain of group 3 and 4 revealed improvement of secretory portion architecture in addition to decreased vaculation and pleomorphism than group 2. There were a high statistical significant difference, of caspase 3 immuno-expression, between the treated chitosan and curcumin groups (group3 and 4) and MSG group (group 2) as p values were 0.005 and 0.017 respectively.<br /> Conclusion: administration of chitosan or curcumin has a protective effect on SMG toxicity induced by chronic intake of MSG.https://edj.journals.ekb.eg/article_159752_b6a24ae96abdbac4cea469010c6caedd.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401The Anti-Inflammatory Potency of Vitamin E and Coconut Oils on Ligature-induced Periodontitis in Albino Rats (Histological and Immunohistochemical Study)1199121615975410.21608/edj.2021.47926.1318ENHodaDesoukyDepartment of Oral Biology, Faculty of Dentistry, Misr International University, Cairo, EgyptDinaGomaaLecturer, Department of Oral Biology, Faculty of Dentistry, Ain Shams UniversityMohamedZayedAssociate Professor, Department of Oral Biology, Faculty of Dentistry, Misr International UniversityAhmedHalawaProfessor, Department of Oral Biology, Faculty of Dentistry, Ain Shams UniversityJournal Article20201028The aim of this study was to evaluate the anti-inflammatory potency of vitamin E and coconut oils when used for oil gum massage therapy in periodontitis and compare them to the conventionally used chlorhexidine. Methods: 105 adult male albino rats were divided into 5 groups of 21 rats each: The negative control group (group 1) receiving no intervention, the positive control group (group 2) receiving ligature induced periodontitis without any treatment, the chlorhexidine experimental group (group 3) that received ligature induced periodontitis and chlorhexidine for 10 days, the vitamin E experimental group (group 4) that received ligature induced periodontitis and vitamin E for 10 days. And the coconut oil group (group 5) that received ligature induced periodontitis and coconut oil for 10 days. Groups 2-5 were further divided into subgroups A,B, and C according to day of termination (day 3, 7, &10). After rats were terminated, maxillary molar regions were excised and stained with H&E and Masson’s trichrome stain. Specimens were also examined immunohistochemically for the expression of MMP-1 antigen. Results: Histological and immunohistochemical examination showed an inflammatory reaction with ligature induced periodontitis that decreased gradually from day 3 until day 10. Statistical analysis of immunohistochemical expression of MMP-1 showed decrease over the course of treatment in all experimental groups, chlorhexidine showing the greatest decrease in inflammation and no significant difference between vitamin E and coconut oil. Conclusion: Oil gum therapy using either chlorhexidine, vitamin E, or coconut oil was associated with variable grades of statistically significant reduction of inflammation.https://edj.journals.ekb.eg/article_159754_fab22523383c2eef63beae2ead1ff014.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401The Effect of Type and Dose of Anti-Inflammatory Drugs on the Viability and Osteogenic Potential of Dental Pulp Stem Cells1217122715982710.21608/edj.2021.52035.1381ENMohamedRefaatDentist, Egyptian Ministry of Health And Population, Mansoura, Egypt0000-0003-3085-478XMenatallaElhindawyLecturer of Oral Biology, Oral Biology Department, Faculty of Dentistry, Mansoura University, Egypt0000-0001-8866-1564MohamedHelalProfessor of Oral Biology, Oral Biology Department, Faculty of Dentistry, Mansoura University, EgyptJournal Article20201202• Objectives <br /> The use of nonsteroidal anti-inflammatory drugs (NSAIDs) after regenerative surgeries may affect the behavior of stem cells used during the treatment. This study investigates the possible effect of NSAIDs on the proliferation and the osteo-differentiation of dental pulp stem cells (DPSCs) in vitro. <br /> • Methods <br /> DPSCs were isolated, cultured and characterized with human antibodies CD90, CD105 and CD45. Cells were cultured for a period of 1, 3 and 5 days to assess the viability using different drug concentrations of acetylsalicylic acid (ASA) 10, 50, and 200 μg/mL., Diclofenac sodium 10-4 and 10-6 M and Meloxicam 0.01, 0.1, and 1 μM .Selected concentration of ASA 10 μg/mL, Diclofenac sodium 10-6 M and Meloxicam 0.1 μM were used during osteo-differentiation of the cells.<br /> • Results <br /> DPSCs viability assay recorded the highest absorption rate on the 1st day with ASA 10 μg/mL and on the 3rd day with Meloxicam 0.01 μM. On day 5 all concentration of ASA and Diclofenac reported the highest absorption rats. The highest values of calcium compounds was expressed with ASA 10 μg/mL on the 7th day using alizarin red satin.<br /> • Conclusion <br /> Using different types of NSAIDs during the postsurgical regimen can affect the viability and osteo-differentiation of DPSCs.https://edj.journals.ekb.eg/article_159827_cf0d2e56dea188a374579ba5b8768e03.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Maxillary sinus lift to enable one stage implant placement using Nanobone & PRF with or without simvastatin.1229123915982510.21608/edj.2021.56278.1442ENOmarSolimanLecturer, Periodontology, Oral Medicine and Oral Diagnosis Department, Faculty of Dentistry South Valley
University0000-0002-0122-4490Journal Article20210102Objective: Aim of this study was to evaluate the effect of using Nanobone & PRF with or without simvastatin on sinus lift and one stage implant placement.<br /> Material & Methods: 20 patients need sinus lift for implant placement in atrophied posterior maxilla were divided in two groups (group I: maxillary sinus left made by using synthetic bone substitute (NanoBone) & PRF Without simvastatin, while in group II using NanoBone & PRF with simvastatin. <br /> Results: Six months after surgery the mean of bone height gain in group I was 11.349 mm and the mean bone density was 297.8 Hounsfield units, while in group II the new bone height was 11.889 mm and the mean bone density was 314.3 Hounsfield units. During and after surgery there were no major complication and no implant failure. For endo sinus bone gain results were statistically significant in favour of group II (P<0.05).<br /> Conclusion: Both regenerative approaches showed significant improvements in clinical and radiographic results. However, adding simvastatin in combination with Nanobone & PRF showed better bone gain at 6 months in maxillary sinus lift and implant placement.https://edj.journals.ekb.eg/article_159825_1830919845dab396ad8b811d04aba73b.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Reosseointegration of bone defect surrounding dental implant associated with periimplantitis using Nanobone with or without simvastatin: A six month randomised controlled clinical trial.1241125015982810.21608/edj.2021.56962.1443ENOmarSolimanLecturer of Oral Medicine , Periodontoloy and Oral Diagnosis، Faculty of Dentistry, South Valley University0000-0002-0122-4490Journal Article20210107Abstract:<br /> Objectives: The aim of the study was evaluating the effect of adding simvastatin to synthetic bone substitute on the regenerative surgical treatment of bone defects associated with periimplantitis in a six months randomized controlled clinical trial.<br /> Material & Methods: 30 patients diagnosed with periimplantitis divided in two groups, group I treated by using synthetic bone substitute (Nanobone), group II treated by using Nanobone with simvastatin. Clinical evaluation was the measurements for probing pocket depth (PPD), clinical attachment level (CAL), plaque index (PI), modified sulcus bleeding index (mSBI) and mucosal recession. Radiographic bone fill was evaluated at baseline and after six months.<br /> Results: The results for both treatment groups showed significant reductions obtained in the mean PPD, CAL and mSBI at six months postoperatively when compared to baseline values , on the other-side no statistically significant difference was observed for the mean PI and MR. The mean of bon fill in group II was better than that of group I and this results was statistically significant (P < 0.05).<br /> Conclusions: The two regenerative approaches produced significant improvements in both clinical and radiographic assessments, but adding simvastatin to Nanobone produce better results in terms of bone fill.https://edj.journals.ekb.eg/article_159828_169f04a7860cdc6b84c6e336bfe8d87a.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Immunohistochemical expression of sonic hedgehog signaling and its influence on angiogenesis in osteosarcoma and chondrosarcoma1251126015982910.21608/edj.2021.57745.1451ENShaimaaGhazyLecturer of Oral Pathology, Faculty of Dentistry, Ain Shams University.https://orcid.org/00SehamAbdel GhaniLecturer of Oral Pathology, Faculty of Dentistry, Ain Shams University.0000-0001-8100-3833Journal Article20210112Abstract<br /> Review: Osteosarcoma is considered a primary cancer type of bone neoplasms with locally destructive behavior. Chondrosarcoma is a malignant neoplasm of bone in which there is a production of cartilage matrix by the neoplastic cells. Hedgehog pathway activation is important in osteoblast and chondroblast differentiation. Abnormal activation of the Hh pathway results in malignant neoplasms formation such as stomach cancer, breast, intestinal cancer and prostate cancer. Many researches assume the participation of Hh activation in cancer-related neovascularization. VEGFR2 is a type V receptor tyrosine kinase which is seen in vascular endothelial cells. The receptor is activated when attached to its (VEGF) ligand, which starts a phosphorylation steps that finally stimulates activation of endothelial cell growth and migration.<br /> Aim of study: The current study was performed to examine the immunohistochemical presence of SHH in both osteosarcoma and chondrosarcoma and correlate its presence with angiogenesis, which has a main part in the spread and invasion of cancer. <br /> Material and Methods: Immunohistochemical expression of SHH and VEGFR2 was evaluated in 10 samples of jaw osteosarcoma cases and 10 samples of jaw chondrosarcoma.<br /> Results: Osteosarcoma expression for both SHH and VEGFR-2, was significantly higher compared to chondrosarcoma with non statistically significant positive correlation between SHH and VEGFR-2 (P-value >0.001) in both lesions .<br /> Conclusion: The over expression of SHH in both osteosarcoma and chondrosarcoma indicates its important role in carcinogenesis of these tumors . The positive correlation between SHH and VEGFR2 donates the crucial role of SHH in activation of angiogenesis.https://edj.journals.ekb.eg/article_159829_b5a518ed90408a11afaa14144565e71c.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Correlation between epithelial-mesenchymal transition and collagen quality in oral dysplasia and squamous cell carcinoma1261127415983010.21608/edj.2021.58518.1459ENNermeen SamiAfifiLecturer in Oral Pathology Department, Faculty of Dentistry Ain Shams University0000-0003-0271-3656Rehab FouadFathiLecturer in Oral Pathology Department, Faculty of Dentistry, Ain Shams University0000-0002-6969-9011Shaimaa MustafaMasloubLecturer in Oral Pathology Department, Faculty of Dentistry, Ain Shams University0000-0001-8413-4863Journal Article20210121Epithelial-mesenchymal transition (EMT) enhances the invasive ability, providing an explanation for the mechanism of tumor metastasis. The stiffness of the stromal extracellular matrix collagen can regulate the epithelial cell growth and migration in oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC). Objectives: to study the epithelial expression of Vimentin as a marker for EMT and to elucidate the nature of collagen in OED and OSCC and finally to correlate EMT to collagen quality in these lesions. Material and methods: the area fraction of immunohistochemical epithelial expression of Vimentin and color polarization of picrosirius red stained collagen fibers were detected in ten specimens of each grade of OED and OSCC as well as normal oral mucosa followed by statistical analysis. Results: a statistically significant increase in the median area fractions of epithelial Vimentin expression and yellow-green fibers were detected as the grade of OED and OSCC progressed. Spearman’s correlation coefficient revealed a statistically significant direct correlation between epithelial Vimentin expression and yellow-green fibers in these lesions. Conclusion: there was a statistically significant increase in EMT and decrease in the collagen quality with the advance of OED and dedifferentiation of the neoplasm. The significant inverse correlation between EMT and collagen quality indicates the role of EMT in stromal collagen quality in these lesions and aid in their prognosis.https://edj.journals.ekb.eg/article_159830_7a6cb6d226f982f664a419b00154d091.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401COVID-19 and the ethical challenges of management in Private Dental Clinics in Egypt.1275128515983210.21608/edj.2021.59887.1468ENFawzyDarweeshAssistant Professor, Oral Biology Dep. Faculty of Dentistry, Zagazig UniversityHamdyMarzookAssociate Professor, Department of Oral Surgery, Faculty of Dentistry, Mansoura UniversityAbeerElgendyAssociate Professor, Department of Endodontic, Faculty of Oral and Dental Medicine, Zagazig UniversityBassantMowafyAssistant Professor, Oral Radiology Dep. Faculty of Dentistry, Mansoura UniversityEmanYousefAssociate Professor, Department of Oral Surgery, Faculty of Dentistry, Mansoura UniversityAhmedSalemAssociate Professor, Department of Oral Surgery, Faculty of Dentistry, Mansoura UniversityJournal Article20210126Background: One of the challenges that face effective dental and oral surgical treatment is reducing transmission of infectious diseases. This study assessed the challenges and the changes in management of patients in private dental clinics in Egypt in view of rapid spread of SARS-COVID-19.<br /> Methods: Six private dental clinics in Egypt were enrolled in this study. Changes in the management protocols were recorded and evaluated. Data were collected about the changes in clinic policies and in infection control practices. The modifications in patient management and the number and types of treatment approaches either surgical or non-surgical for dental and oral conditions were recorded and evaluated. <br /> Results: The most commonly followed guidelines were wearing gloves followed by wearing masks and using disposable products. Remote consultations and shortage of personal protective equipment were recorded by all clinics. Management approaches included conservative endodontic management for periapical lesions and fistulae. Aerosols and minor surgical interventions could not be avoided in many cases. <br /> Conclusion: Conservative approaches were effective and could be considered during pandemic spread. Dental personnel should be more aware and more prepared for any pandemic spread. Management modifications and strict precautions should be followedhttps://edj.journals.ekb.eg/article_159832_e58eca781b531ccb761f1517dd645c61.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401CRIBRIFORM ADENOCARCINOMA OF MINOR SALIVARY GLANDS: A CASE REPORT WITH FOCUS ON RADIOGRAPHIC APPERANCE1287129015983310.21608/edj.2021.64861.1525ENMazenAldosimaniAssistant Professor, Department of Oral Medicine and Diagnostic Sciences, Division of Oral and Maxillofacial
Radiology, College of Dentistry, King Saud University, Riyadh, Saudi Arabia0000-0002-4105-2283Journal Article20210224Cribriform adenocarcinoma of minor salivary gland (CAMSG) is currently classified as a subtype of polymorphous adenocarcinoma. CAMSG differs from polymorphous adenocarcinoma in that it has a distinct histopathologic appearance and clinically CAMSG has higher rate of lymph node metastasis at initial presentation. Panoramic radiograph shows an ill-defined radiolucent lesion in the right maxilla that extends from the distal of maxillary right canine to the mesial of maxillary second molar. The lesion has invasive borders and causes destruction and loss of the lamina dura and periodontal ligament space in the mesial of the second molar and distal of the canine. The lesion has also invaded into the right maxillary sinus destroying the floor of sinus. CBCT images show a low attenuation lesion involving the right maxillary alveolar bone at the premolars and first molar area. This report describes the radiographic appearance of a case of CAMSG on panoramic radiograph, cone-beam computed tomography (CBCT), multi-detector computed tomography (MDCT) and on magnetic resonance imaging (MRI).https://edj.journals.ekb.eg/article_159833_bd57007cdea64776f889c6f1a53fd6e1.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Influence of orthodontic mini-screw surface modifications on implant topography and osseointegration: an in- vivo study.1291130515983410.21608/edj.2021.63997.1512ENRehab RizkEL-ZeharyAssociate Professor, Oral Biology Department, Faculty of Dentistry, Mansoura University, Egypt.0000-0002-1265-6459Marwa ATawfikAssociate Professor, Orthodontic Department, Faculty of Dentistry, Mansoura University, Egypt.0000-0002-5109-4313Samah IMouradAssociate professor, Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Mansoura University, Egypt.Enas TEnanAssociate professor, Dental Biomaterials Department, Faculty of Dentistry, Mansoura University, Egypt.0000-0002-5277-461XJournal Article20210219ABSTRACT<br /> Introduction: Implant-supported orthodontic treatment has gained an expanded interest by researchers and orthodontists in the recent years. Therefore, different methods were tried to achieve the best results in this kind of treatment. <br /> Aim of the study: The current research was conducted to compare the effect of 2 different treatment methods on the surface of orthodontic titanium mini-screws and on bone response to the treated implants. <br /> Materials and methods: Forty-eight titanium mini-screw implants were distributed under 3 equal groups; as received, sandblasted, and laser-treated, respectively. After application of the surface-treatment methods, half of the selected screws were tested for surface morphology, using a scanning electron microscope (SEM) and a digital profilometer. To evaluate the biological effects of surface treatment methods, the other screws were implanted in rabbits’ tibias which were subjected to histological examination at the end of the experimental periods of 4 and 8 weeks. The surgical procedures and euthanasia were conducted according to the limitations of Research Committee of Mansoura University.<br /> Results: Sandblasted implants showed the highest surface roughness and the most pronounced bone response. <br /> Conclusion: Sandblasting surface treatment provide more favorable conditions for implant osseointegration, in comparison to laser-treatment.https://edj.journals.ekb.eg/article_159834_b6a98cc6697b8c1a13a2085ae4727296.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Influence of Surface Resin Coating on Bio-Compatibility and Attaching ability of Human Gingival Cells to the Glass-Ionomer Restorative Material1307131815983610.21608/edj.2021.64051.1514ENMonaDenewarLecturer of Oral Biology, Faculty of Dentistry, Mansoura UniversityKhalid M.AbdelazizDepartment of Restorative Dental Sciences, College of Dentistry, King Khalid University, Abha, Saudi ArabiaMahitabeElgamilyLecturer of Oral Biology, Faculty of Dentistry, Mansoura UniversityJournal Article20210223Background and objective. Glass-Ionomer (G.I.) restoratives are used to manage cervical tooth defects; however certain chemicals could leach out of the material and affect the adjacent gingival tissue. Therefore, this study aimed to assess the viability and attachment of human gingival epithelial cells (HGECs) on both resin-coated and uncoated Equia fil G.I. restorative materials. <br /> Materials and Methods. Two groups of G.I. disc specimens (n= 34 each) were constructed from Equia fil G.I. restorative by mixing the content of G.I. capsules, then injection into silicone molds, the material was left for setting before cutting the excess. Surfaces of group I specimens were coated with the accompanied protective Equia resin, while those in group II were left uncoated. HGECs were isolated and incubated with the constructed specimens. The cell viability was then assessed using both Methyl Tetrazolium (MTT) and Trypan blue dye exclusion (TBDE) assays. The micromorphology and the attaching ability of the isolated cells to specimens’ surfaces were assessed using SEM. The surface roughness was measured and the chemical analysis of G.I. surfaces was also analyzed by energy disperse x-ray.<br /> Results. Reduction in HGECs viability was noticed in contact with G.I. of both test groups. However, HGECs showed better viability and attaching ability to the resin-coated G.I. surface than were in presence of uncoated restorative.<br /> Conclusion. Resin Coating preserves the cell viability and improves the attachment of HGECs on Equia fil G.I restorative surfaces. This finding, therefore, offers sound evidence to select such material in treating sub-gingival cervical tooth defects.https://edj.journals.ekb.eg/article_159836_ab3fe9c3b283908e10bfa8792318fa0e.pdfThe Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948467220210401Odontometric analysis using CBCT for sexual dimorphism in Egyptian-Fayoum population in case of normal occlusion1319133215983710.21608/edj.2021.66478.1542ENEngy Ali AbdelhaleemSalamOral radiology department, faculty of dentistry, Fayoum University0000-0001-5320-5331Ayman RaoufKhalifaDepartment of Pedodontics and Orthodontics, October 6 University, Faculty of Dentistry, Cairo, Egypt0000-0003-3269-3051Dina MohamedHassounaLecturer of Oral Biology- Faculty of Dentistry- Fayoum University0000-0002-4244-9792Journal Article20210306Objectives of this study was to evaluate odontometric linear measurements obtained from CBCT software including mesiodistal, tooth length dimensions and enamel thickness of the mandibular canine and mandibular first molar and arch measurements for sexual dimorphism in Egyptian population sample. Material and Methods: The study was carried out on 100 patients who visited the department of oral and maxillofacial radiology, Fayoum University for dental treatments. The study sample were grouped into two groups, group A (50 males) and group B (50 females) aged between 18-25 years old. The teeth which were selected for linear measurements using CBCT in the present study were 100 mandibular permanent canines (Lower 3) and 100 mandibular permanent 1st molar (Lower 6), for each group respectively. The linear measurements were taken on crown width and height, Enamel thickness, arch depth and width. Results: There was a highly significant difference between Male and Female for odontometric measurements of mandibular right and left canines and 1st molars including (CWL3, CLL3, CWL6 and CLL6) while enamel thickness measurements showed non-significant differences between males and females including (ETL3 at cusp tip, ETML6, ETDL6 and ETL6 at mesio-lingual cusp tip). Arch measurements including (ICW, IMW through fossa and IMW through contact) showed high significant differences between males and females. Conclusion: Odontometric parameters including mandibular canine, mandibular first molar crown width and length, inter-canine width and inter-molar width measurements based on CBCT imaging show highly significant gender difference. Thus, the parameters can be simple and reliable sexual dimorphism for medicolegal purpose.https://edj.journals.ekb.eg/article_159837_2cb4181483b0a5bbf9b178edc7062c5d.pdf