The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Ultra-Low-Dose versus Normal-Dose Scan Protocol of Planmeca ProMax 3 D Mid CBCT machine in Detection of Second Mesi buccal Root Canal in Maxillary Molars: An ex vivo study2212297140510.21608/edj.2015.71405ENDina F.AhmedLecturer of Oral and Maxillofacial Radiology, Faculty of Dentistry, Cairo University.Journal Article20200216Objective: The aim of this study was to compare the ability of Normal and Ultra-Low-Dose protocols of Planmeca Promax 3 D Mid CBCT unit with different voxel sizes in detection of MB2 canal.<br />Materials and Methods: This study was conducted on 36 extracted human maxillary first and second molars. Six acrylic models were prepared and six molars were embedded in each model using Inlay wax. Each acrylic model was then placed in a plastic container filled with water. CBCT imaging of the acrylic models containing the extracted molars was taken by Planmeca ProMax 3D Mid machine (Helsinky, Finland). Each model was scanned with three different voxel sizes (0.075, 0.1, 0.2 mm) and two different exposure protocols, namely the Normal-Dose (ND) and the Ultra-Low-Dose (ULD) protocol. Assessment of the number of MB canals was carried out by two Oral and Maxillofacial Radiologists. To verify the number of root canals of the mesiobuccal root, these roots were sectioned and visually inspected. The data obtained were tabulated and subjected to statistical analysis.<br />Results: Transverse sections showed that 22 molars had two mesiobuccal root canals and 14 molars had only one mesiobuccal root canal. The results showed that the sensitivity and accuracy of CBCT images increase as the voxel size decrease. However, the results revealed no significant difference (p>0.05) between the (ND) and (ULD) protocol of the same voxel size used. Both intra and inter-observer reliability showed very good agreement (0.81–1).<br />Conclusions: ULD CBCT protocol can be applied for the detection of MB2 of maxillary molars. The smaller the voxel size, the higher the image resolution and image quality. The 0.075 mm voxel size of both protocols is accurate enough to be used as a gold standard in laboratory studies instead of the standard root sectioning technique.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Effect of Metronidazole Loaded Nanoparticles on Treatment of Induced Periodontal Defects in Dogs2312407140710.21608/edj.2015.71407ENEnas AhmedElgendyAssociate Professor of Oral Medicine, Periodontology and Oral Diagnosis. Faculty of Dentistry, Kafr El-Sheikh University. Egypt..Amel Mohamed EzzatAbd-ElhamidAssistant Professor of Oral Biology, Dental Faculty, Tanta University. Egypt.Alaa M. MetwalliMoustafaAssistant Professor of Surgery, Anesthesia and Radiology, Faculty of Veterinary Medicine, Kafr El-Sheikh University, EgyptTamerElamer Abo ShadyLecturer of Oral Medicine, Periodontology and Oral Diagnosis. Faculty of Dentistry, Tanta University. Egypt.Journal Article20200216Objective: The aim of the present study was to compare between 25% metronidazole gel and 25% metronidazole loaded nanoparticle gel formulation on healing of experimentally induced periodontal defects in dogs.<br />Materials and Methods: Periodontal defects were induced by ligatures placement in 10 beagle dogs in both lower sites. The defects were randomly and equally allocated in a split-mouth design to either Metronidazole gel nanoparticles gel (25%) (Experimental site group I) or Metronidazole gel (25 %) (Control site group II) The animals were sacrificed with an overdose of anesthesia at 8 weeks and block sections of the defects were collected for histological and histometric analysis.<br />Results: The results showed improved outcomes in terms of bone as well as new cementum and functionally oriented periodontal ligament formation. However, sites treated with metronidazole loaded nanoparticles (25%) gel formula (experimental group I) showed significantly greater regenerative potential of the attachment apparatus in terms of newly formed cementum and bone compared to conventional metronidazole gel treated group.<br />Conclusion: This, in turn, proves that 25% metronidazole loaded nanoparticle gel is more efficient compared to conventional metronidazole gel in treatment of periodontal defects. Clinical Relevance: <br />Scientific Rationale for Study: Evaluating the effect of metronidazole loaded nanoparticles on the treatment of induced periodontal defects in dogs. Principal Findings: Findings from this animal study of 10 beagle dogs supported the potential of metronidazole loaded nanoparticles gel, as compared to 25% metronidazole gel, for providing a safe, inexpensive, and effective therapy for induced periodontitis by regenerating new bone as well as new cementum and forming functionally oriented periodontal ligaments. Practical Implications: Metronidazole loaded nanoparticles gel can be used as adjunctive treatment of periodontitis.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Correlation between Inflammatory Signals and Tumor Associated Macrophages in Oral Squamous Cell Carcinoma2412527140810.21608/edj.2015.71408ENSarahAdolf BarsoomTeaching Assistant of Oral Pathology, Faculty of Oral and Dental Medicine, Future University in EgyptHoury MoustafaBaghdadiProfessor and Head of Oral Pathology Department, Faculty of Dentistry, Ain Shams UniversityHala A.El-kammarLecturer of Oral Pathology, Faculty of Oral and Dental Medicine, Future University in Egypt.Nermeen SAfifiLecturer of Oral Pathology, Faculty of Dentistry, Ain Shams UniversityJournal Article20200216Background: Squamous cell carcinoma grows in a complex and dynamic stroma. This microenvironment provides all the necessary stimuli for tumor viability, growth and invasiveness. COX-2 is upregulated during both inflammation and cancer. CD163, a marker of M2 macrophages (Tumor associated macrophages- TAMs), has been studied in several aggressive tumors. The increased expression of CD163 was associated with a poor overall survival in various cancers. <br />Aims of the Study: The present study aimed to correlate the inflammatory signals presented by COX-2 immunoexpresion with tumor associated macrophages expressing CD163 in different grades of OSCC. <br />Materials and methods: Immunohistochemical expression of COX-2 and CD163 was evaluated in 30 cases of different grades of OSCC. <br />Results: The mean difference between the well-differentiated level and the moderately differentiated level of COX-2 and CD 163 was statistically insignificant, while that between the well- and poorly differentiated and between the moderately and the poorly differentiated were highly statistically significant. <br />Conclusions: inflammatory signals presented by COX-2 immunoexpression and TAM (CD163+ cells) were elevated with increased tumor grade.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Coenzyme Q10 Food Supplement on the Treatment of Chronic Periodontitis in Patients with Type II Diabetes Mellitus: A Randomized Control Study2532617140910.21608/edj.2015.71409ENMalak Youssef MohamedShoukhebaAssociate Prof. of Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Tanta University, EgyptSoheir El-Sayed MohammedEl- KholyLecturer of Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Tanta University, EgyptJournal Article20200216The ultimate goal of the present study was, to evaluate the effectiveness of Coenzyme Q10 (CoQ 10) supplementation as an adjunct to scaling and root planing in reducing gingival inflammation and periodontal tissue destruction in chronic periodontitis patients with type II diabetes mellitus (DM).<br />Material and Methods: Thirty patients with chronic periodontitis and type II DM were equally divided into 2 groups. Group I received scaling and root planing plus placebo capsule for 3 months and groups II received scaling and root planing and Coenzyme Q10 for 3 months. Bleeding on probing and gingival index were taken at baseline,1, 3 and 6 months’ evaluation periods while probing pocket depth, clinical attachment loss at baseline, 3 and 6 months only. Gingival crevicular fluid (GCF) samples were collected for measuring matrix metalloproteinase -8 (MMP-8) in GCF at baseline,1, 3 and 6 months’ evaluation periods.<br />Results: Statistical analyses demonstrated a significant reduction in clinical parameters from baseline to 6 months in group II (test group) as compared to their baseline value P<0.001, while control group showed improvement in clinical parameters up to 3 months only followed by statistically insignificant increase in the mean scores of the tested parameters at 6 months as compared to baseline values P>0.05. Intergroup comparison showed statistically significant differences between the two studied groups at all the study evaluation periods in favor to group II (test group). GCF-MMP-8 levels showed statistically significant continual reduction from baseline up to 3 months followed by slight increase at 6 months which is still below baseline value for both group PConclusions: from the results of the present study it is clear that dietary supplementation with CoQ 10 may provide a low-cost intervention to augment periodontal therapy. Hence, CoQ 10 as an antioxidant could be used safely as an effective adjunct to oral prophylaxis in treatment of chronic periodontitis in diabetic patients.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Evaluation of TMJ Osteoarthritic osseous changes using Different Imaging Modalities: A diagnostic accuracy study2632697141210.21608/edj.2015.71412ENImanDakhliAssociate Professor of Oral and Maxillofacial Radiology, Faculty of Dentistry, Cairo University, EgyptJournal Article20200216Aim: This study was conducted to compare the diagnostic accuracy of CBCT imaging to digital panoramic imaging in assessment of osteoarthritic TMJ osseous changes. <br />Methods: 25 patients were enrolled in the current investigation, divided into two groups, Group (1) the diseased group including fifteen patients. Group (2) the control group including Ten normal asymptomatic healthy patients (20 joints) but they were seeking for treatment of other dental problems, in the two groups enrolled in this study all participants were females with ages ranging between 25-55 years. All participants were scanned with panoramic and cone beam computed tomography (CBCT). <br />Results: ROC curve analysis of panoramic views showed diagnostic accuracy 76.4% while diagnostic accuracy of CBCT (TMJ views) was 81.3% and CBCT (Sagittal views) showed 82.6% diagnostic accuracy. <br />Conclusion: Panoramic images can be used as a preliminary valuable tool for evaluation of osteoarthritic osseous changes equivalent to CBCT images as it revealed relatively high sensitivity compared to CBCT images.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Accuracy of cone-beam computed tomography (NewTom- Giano) in detecting alveolar bone Fenestrations with different Voxel sizes2712757141310.21608/edj.2015.71413ENAhmed MohamedHossamLecturer of Radiology. Faculty of Dentistry October University of Modern Sciences and Art. EgyptJournal Article20200216Objectives: The purpose of this study was to evaluate the accuracy and reliability of cone-beam computed tomography (CBCT) in the diagnosis of fenestrations defects with different voxel sizes. <br />Material and Methods: 128 Defects were created in eight dry skulls with a diameter of one, two, three and four millimeters on the buccal aspect, respectively in each quadrant. Measurements were obtained using a digital caliper. These were considered to be the gold standard. The skulls were scanned by CBCT (NewTom- Giano) at two settings: (a) Voxel size 0.3 mm Standard Resolution (SR) (b) voxel size 0.125 mm High Resolution (HR). The accuracy of the CBCT measurements was determined by comparing the mean of all radiographic measurements, using SAS software version 9.4 for data analysis with those of direct measurements and Duncan’s Multiple Range Test. <br />Results: Statistical analysis for both SR and HR with the gold standard measurements for each defect on the skulls revealed statistically significant correlation between the radiographic measurements and real measurements (P ≤ 0.05) indicating accuracy, also no statistically significant difference was found within the measurements accuracy of each group (P > 0.05) with the different defects sizes. Duncan’s multiple range test revealed that the HR voxel size 0.125 mm is of slightly higher accuracy. <br />Conclusion: Based on the results of the present study, CBCT (NewTom- Giano) has demonstrated high accuracy and reliability, in measuring different fenestration sizes with different resolution (HR 0.0125 mm and SR 0.300 mm voxels). Measurements were with same accuracy despite the slightly higher performance of HR (0.125 mm voxel)The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Tumor necrosis factor alpha and keratin 17 expression in oral submucous fibrosis in rat model2772887141410.21608/edj.2015.71414ENGhada A.Abd El LatifDepartment of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismalia , EgyptJournal Article20200216Background and Objective: Oral submucous fibrosis is an inflammatory and a premalignant lesion as classified by “World Health Organization”. Its pathogenesis is related to collagen metabolism. Bleomycin is an anti-carcinogenic drug, but it causes pulmonary fibrosis. This study was carried out to study the role of tumor necrosis factor alpha and keratin 17 in pathogenesis of oral submucous fibrosis. <br />Study Design: Fifty pathologically-free female rats were divided into one control group and four test groups. They were injected by phosphate-buffered saline and Bleomycin into the buccal mucosae daily for 2, 4, 6 and 8 weeks, respectively. The histopathological changes was evaluated by studying the changes within epithelium tissue and lamina propria by (H&E) and the expression of both (tumor necrosis factor alpha and keratin 17) using immunohistochemistry and enzyme linked immunosorbent assay respectively. <br />Results: The buccal mucosae of the test groups in comparison with control showed hyperplastic and hyperatrophic epithelial changes that gradually decreased with the severity of oral submucous fibrosis, with epithelial atrophy and focal dysplastic changes, which increase with severity of oral submucous fibrosis gradually. The lamina propria showed gradual increase in inflammatory cells, collagen formation and closure of the blood vessels. Regarding tumor necrosis factor alpha the area percent of immunoexpression, showed the lowest expression in the control and test groups showed gradually increase after 2, 4, 6,8 weeks within epithelium , on the other hand, the area percent of immunoexpression of tumor necrosis factor alpha within lamina propria showed gradually increase after 2, 4, 6 weeks, then decreased after 8 weeks. Regarding Keratin-17, the lowest value was recorded in control group while it increased gradually from the second week to the 8th weeks.<br />Conclusion: Both protein molecules tumor necrosis factor alpha and keratin 17 appear to be related in case of oral submucous fibrosis. As tumor necrosis factor alpha and keratin 17 are interdependent regulators, they could be used as diagnostic makers and a prognostic mirror of oral submucous fibrosis cases. Tumor necrosis factor alpha inhibitory drugs can be targeted in new therapeutic agents to improve the prognosis of oral submucous fibrosis.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101BONE REGENERATION AND SOCKET PRESERVATION USING BONE AUGMENTING MATERIALS (HISTOMORPHOMETERIC STUDY IN RABBITS)2892997141610.21608/edj.2015.71416ENEmanKhalilLecturer of Oral Medicine, Periodontology, and Oral Diagnosis, College of Oral and Dental Surgery, British
University In Egypt (BUE), EgyptMaha Galal A.OmarProfessor of Oral Medicine, Periodontology, and Oral Diagnosis, College of Dentistry, Princess Nourah Bint
Abdulrahman University. Riyadh, Saudi ArabiaJournal Article20200216Background: The ultimate goal of periodontal therapy is the regeneration of tissues destroyed by periodontal disease Fibronectin belongs to a group of high molecular weight glycoproteins that exist on cell surfaces. It is known to play a role in cell-to-cell and cell-to-substrate adhesion, as well as an essential role in osseointegration due to its capacity to make osteoblasts attach to the extracellular matrix(ECM) components. Combination of Fibronectin and commercially available bone grafts may hold a promising potential of enhanced bone regeneration.<br />Objective: Comparison of the effect of fibronectin, Bovine-Derived Xenograft (BDX)and their combination for socket preservation through histologic and histomorphometric analysis.<br />Material and Method: Thirty six clinically healthy adult New Zealand white male rabbits, were used in this study. The animals were equally divided into three groups; I-control group (defect was left empty as control), II- bio group (bio- oss was applied on the socket ) and III-( Bio Fibro group) bone and fibronectin group( socket was filled with Bovine-Derived Xenograft Bio-Oss® with fibronectin). At 4 and eight weeks the animals were sacrificed. Bone regeneration among the three groups was evaluated through histologic and histomorphometric analysis.<br />Results: The most significant value of area percent of newly formed bone was observed in the (bone and fibronectin) group, then the fibronectin group, with the least amount recorded in the control group. The difference was extremely statistically significant (p<0.0001). The socket is almost filled with newly formed bone trabeculae with organized fibrous tissue in the fibronectin and bone group ( Bio Fibro group ) at eight weeks.<br />Conclusion: Fibronectin is a useful biomaterial for the enhancement of bone formation. Combination of fibronectin and bovine graft seems to lead significantly to favorable bone regeneration and could be efficaciously used in the socket preservation.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Effect of Black Raspberry on NF-κB and Caspase-3 Expression in Head and Neck Squamous Cell Carcinoma versus keratinocytes and fibroblasts cell lines3013087141710.21608/edj.2015.71417ENShaimaa EliwaGhazyLecturer of Oral Pathology, Faculty of Dentistry, Ain Shams University.Dina Hazem HassanGomaaLecturer of Oral Biology, Faculty of Dentistry, Ain Shams University.Journal Article20200216Review: Oral squamous cell carcinoma (OSCC), the most common type of intraoral cancers, is an important health issue. Most cytotoxic drugs have side effects on normal tissues beside being cytotoxic to malignant cells. Many studies demonstrated the ability of black raspberries (BRBs) to inhibit many types of cancer. However, understanding how the bioactive compounds in BRBs drive the metabolic and molecular pathways that lead to oral cancer chemoprevention remains unclear.<br />Aim of study: The current study aimed to investigate the consequences of BRBs treatment on (NF-κB) and caspase-3 expression in two types of head and neck SCC cell lines in comparison to both normal keratinocyte and fibroblast cell lines. <br />Results: There was an inhibitory effect of BRBs on both tested malignant cell lines (SCC9), and (HEp-2) and at the same time there was no effect on growth of the tested normal cell lines (HaCaT) and (BJ). There was a statistically significant increase in median fold change of caspase-3 in the tested malignant cell lines and a statistically significant decrease in the median fold change of NF-κB in the tested malignant cells with no detectable change for both antigens in the normal cell lines.<br />Conclusion: The results of the present study provide more evidence that BRBs have a very promising cytotoxic effect on oral cancer cell lines with no detectable harmful effect on normal cells.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Association of Cortactin and MDM2 in Solid Ameloblastoma and its Malignant Counterpart3093187141810.21608/edj.2015.71418ENNadia A.RadiLecturer of Oral and Dental Pathology, Faculty of Dental Medicine for Girls. Al Azhar University0009-0003-8925-7872Journal Article20200216Ameloblastoma is characterized by heterogeneity regarding its pathogenetic development, molecular abnormalities, and clinical course. Determining the molecular basis underlying these important differences remains challenging. Nevertheless, identifying molecules that influence tumor behavior would be the first steps towards gaining some insights into its pathobiology. MDM2, a 90-95kDa protein coded by a mdm2 gene which is mapped on chromosome 12q13-14. MDM2 is a negative regulator of p53 activity, through binding to the transcriptional activation domain of p53 leading to proteasome-mediated degradation, Cortactin, a product of CTTN gene, mapped to human chromosome 11q13 regulates actin-related (Arp2/3) complex induction of actin polymerization during invadopodia formation, thereby promoting cell motility. This has been accomplished through its involvement in all steps of the invadosome lifecycle, from the assembly, maturation, proteolytic activity, and disassembly. This study aims to evaluate the association of both MDM2 and Cortactin expression in solid ameloblastoma and its malignant counterpart and analyzing their significance. 10 cases of SAB with its different histologic variants and 20 cases malignant variants of ameloblastoma tumors were collected. An immunohistochemical investigation using, Cortactin, and MDM2 antibodies were done for all specimens. There was a statistically significant difference between malignant group and benign group. According to the current study Cortactin and MDM2 can be useful when considering the diagnosis of malignancy.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Histological and Ultra structural Study of the Effect of Monosodium Glutamate on the Submandibular Salivary Gland of Adult Albino Rats3193297141910.21608/edj.2015.71419ENHeba FathyEl ImamLecturer of Oral Biology, Faculty of Dentistry, Egyptian Russian University, Cairo, Egypt.Nesreen NabilAbd El SalamLecturer of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, EgyptJournal Article20200216Introduction: Monosodium glutamate (MSG) is used as a food flavor enhancer all over the world. However, it has adverse effects on human and experimental animals at different organs. Aim: This is study designed to evaluate the histological and the ultrastructure effect of two different doses of MSG on submandibular salivary gland (SMG) of albino rats. <br />Materials and Methods: Fifteen adult male albino rats (180 -200 gm). They were randomly divided into three groups (n=5). The control group I: rats were received 1 ml distilled water orally once daily for 8 weeks. The experimental groups, group II, group III: rats were received 15 mg/kg and 30 mg/kg b. wt. respectively of MSG dissolved in 0.2 ml of distilled water orally once daily for 8 weeks. Animals were sacrificed and two pairs of (SMG) were dissected out, left side was processed for light microscopic examination LM and the right one for transmission electron microscopic examination TEM. <br />Results: LM examination of group II revealed some serous acini lost their normal arrangement. The acini were distended, their cytoplasm was vacuolated and some of them were degenerated. Nuclear changes appeared in form of large, hyperchromatic, pleomorphic and some pyknotic nuclei. Dilated intercalated and striated ducts with ill-defined cell borders. Enlarged blood vessels that engorged with red blood cells RBCs found between the acini and the striated ducts. Mucous acini has irregular arrangement with vacuolated cytoplasm and nuclear changes. Group III revealed loss of normal glandular architecture, abnormal shape of serous and mucous acini, obvious nuclear changes. The ductal cells were dilated and blood vessels engorged with RBCs with inflammatory cell infiltration in connective tissue. TEM examination of group II revealed mild changes as irregular outline of electron dense nucleus, occasional confluence of secretory granules. Marked dilatation of ruff endoplasmic reticulum, few number of lysosomes and mild dilatation of intracellular canaliculi. These changes were more obvious at group III. <br />Conclusion: From the present study we concluded that MSG has degenerative changes at acini and ductal cells of (SMG). Therefore, we recommended that excessive consumption of food containing this salt should be avoided.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Glutathione and selenium as chemopreventive agents on experimentally induced hamster buccal pouch epithelial dysplasia3313427142110.21608/edj.2015.71421ENKamal Abd ElrahmanKamalLecturer of Oral Pathology - Faculty of Dentistry - Al-Azhar UniversityAmr SaadAbdoAlwahabAssistant Professor of Oral Biology - Faculty of Dentistry - Al-Azhar UniversityAbd Elnasser Abd El MolaEsmailLecturer of Oral Pathology - Faculty of Dentistry - Al-Azhar UniversityJournal Article20200216The aim of the present study was directed to investigate the effect of Glutathione (GSH) and Selenium (Se) as chemopreventive agents in experimentally induced hamster buccal pouch (HBP) epithelial dysplasia. <br />Material and methods: Twenty five golden Syrian male hamsters were used as experimental animals and divided into five groups (G1, G2, G3, G4 and G5): G1: 5 animals were left untreated. G2: 5 animals, their right buccal pouches were painted with 0.5% 7, 12 dimethylbenz [a] anthracene (DMBA) 3 times a week for 8 weeks. G3: 5 animals were received GSH one week before, as well as during the application of DMPA for 8 weeks. G4: 5 animals were received Se one week before, as well as during the application of DMPA for 8 weeks. G5: 5 animals were received GSH and Se one week before, as well as during the application of DMPA for 8 weeks. After termination of the experiment, all animals were sacrificed, and the buccal mucosa was excised and preparation in order to be examined histologically and immunohistochemically, then statistical analysis based on these examinations was done <br />Results: Regard to expression of Bcl-2, G2 had recorded the highest mean area percentage (44.25%), while G1 had recorded the lowest mean area percentage (6.73%) and there was high significant difference between G1,G2, G3, G4 and G5 where P value was <0.001, while in regard to expression of Bak, G1 had recorded the highest mean area percentage (41.02%), while G2 had recorded the lowest mean area percentage (19.79%) and there was significant difference between G1, G2, G3, G4 and G5 where P value was 0.019. <br />Conclusion: GSH and Se are considered as promising chemotherapeutic agents in prevention of induced HBP epithelial dysplasia and proved important role in apoptosis and proliferation throughout the process of carcinogenesis.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Assessment of IL-17 in Oral Lichen Planus and in Pemphigus Vulgaris3433507142210.21608/edj.2015.71422ENIbrahimEl-RefaiLecturer, Oral Medicine & Periodontology Department, Faculty of Dentistry, Cairo University - EgyptAmiraMagedAssociate Professor, Oral Medicine & Periodontology Department, Faculty of Dentistry, Cairo University - EgyptDinaEl-SaadyLecturer, Dermatology Department, Faculty of Medicine Cairo University-EgyptJournal Article20200216Background: the present study was aimed to evaluate the levels of IL-17 as a core cytokine and a diagnostic marker of both OLP and PV for comparison and evaluation regarding saliva and serum. <br />Subjects and Methods: The present study was performed on a total of 45 subjects divided into 3 groups Group I Fifteen patients diagnosed with OLP Group II Fifteen patients diagnosed with PV with oral lesions and Group III Fifteen healthy patients (control Group). Salivary and serum samples were collected from all participating subjects for determination of IL-17 levels using enzyme-linked immunosorbent assay (ELISA). <br />Results: Mean IL-17 levels in saliva of OLP group were significantly higher statistically than PV patients and control subjects with p values 0.12 and 0.03 respectively. Serum levels of IL-17 in OLP and PV patients were higher compared to control subjects with a statistically significant p value of Conclusion: According to the results the high serum and salivary levels of IL-17 in OLP patients when compared to healthy control group or PV group suggested that it could be used as a diagnostic marker for OLP and could be used to differentiate it from PV.The Egyptian Dental Association (EDA)Egyptian Dental Journal0070-948465Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)20190101Comparative study of the effect of laser irradiation and acid etching surface treatment on bonding of some dental restorative materials To dentin surface3513667142310.21608/edj.2015.71423ENTarek Abdel-HamidHarhashAsst. Professor of Laser Dental Applications, Department of Medical applications of Laser, National Institute for
Laser Enhancement Sciences, Cairo UniversityJournal Article20200216This study was carried out to evaluate the effect of acid etching versus laser etching of dentin surface on the bonding of Composite and Compomer restorative materials to dentin surface by studying microleakage at the dentin-filling interface, compositional analysis of dentin surface, and Scanning Electron Microscopic (SEM) study of the bonding area. 120 freshly extracted premolar teeth were divided according to surface treatment into three groups. Group I (Acid etching group) contained thirty teeth. Twenty teeth were selected to assess microleakage by forming standardized class V cavity on the buccal surface of the selected teeth. Then, teeth were subdivided into two subgroups with 10 teeth in each. Subgroup (i) delivered composite filling while subgroup (ii) delivered compomer filling according to manufacturer instructions. microleakage at the dentin-filling interface of teeth was examined using dye penetration method. dye penetration scores at occlusal and gingival margins were recorded and tabulated. Five dentin surfaces of five teeth from group I were etched by acid then prepared for compositional analysis of dentin surface by Infra-Red (IR) Spectroscopy. Another Five dentin surfaces were etched by acid then prepared for (SEM) assessment. Group II (Laser etching group) contained ninety teeth, twenty teeth were used for assessing microleakage after laser etching taking similar steps to that of acid etching. Thirty dentin surfaces of thirty teeth were etched by laser with different parameters then prepared for compositional analysis of dentin surface by IR Spectroscopy. Thirty dentin surfaces of thirty teeth were etched by laser with different parameters then prepared for SEM assessment. Group III (Untreated group) contained ten teeth that were left untreated. Five dentin surfaces of five teeth were subjected to IR Spectroscopy assessment. Five dentin surfaces of five teeth were prepared for SEM assessment. The results revealed that acid etching of dentin surface resulted in significant less leakage compared to laser etching. IR Spectroscopy revealed changes in dentin surface structure indicating that there was a reaction occurred by the acid and laser etching. SEM assessment showed more openings of dentinal tubules in case of acid etching, while in case of laser etching, there were a surface structural changes with a melting and fusion dentin surface. Acid etching of dentin gives better results on the bonding of Composite and Compomer filling materials than laser etching and thus gives more successful clinical condition. Further investigations are needed by using different types of laser to get more benefit from applications of laser clinically.