Antimicrobial Efficacy of Nanopropolis Coated Vs Silver-Curcumin Nanoparticles Coated Gutta-Percha Points on Various Microbial Species. A Comparative In Vitro Study

Document Type : Original Article

Authors

1 endodontic department, faculty of dentistry, MTI university, Cairo, Egypt

2 microbial chemistry department, national research centre, dokki-12622, cairo,egypt

3 endodontic department, faculty of dentistry, cairo university, egypt

Abstract

Introduction: Nanopropolis, nanosilver and curcumin have a wide antimicrobial action range. They are to be tested as a gutta-percha coating. Objectives: Comparing the antimicrobial efficacy of silver-curcumin nanoparticles versus nanopropolis coated gutta-percha against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Candida albicans and assessing the continuity of both coatings. Materials and Methods: Suspensions of both materials were loaded on filter discs, placed on the surface of agar plates inoculated with tested microbes and incubated for 24 hours. Gutta-percha cones were inserted in Eppendorf tubes containing the tested materials for 24 hours, placed on the surface of agar plates inoculated with the tested microbes and incubated for 24 hours. The antimicrobial activity of the discs was determined by measuring the diameter of the inhibition zone whereas it was measured by positive response and its degree around gutta-percha. Coated gutta-percha cones were scanned under scanning electron microscope to assess the continuity of the coating. Results: Mean and Standard Deviation of the inhibition zone diameter for Silver-curcumin nanoparticles around discs were 12.35±0.71mm for E.faecalis, 20.6±0.39mm for S.aureus, 14.6±0.61mm for E.coli and 18.2±0.67mm for C.albicans. For nanopropolis they were 8.95±0.69mm for E.faecalis, 15.4±0.7mm for S.aureus, 9.75±0.86mm for E.coli and 14.8±0.63mm for Candida. The inhibition zones around gutta-percha were more pronounced for Silver-curcumin nanoparticles. Statistically significant difference was found between the inhibitory effect of silver-curcumin nanoparticles and nanopropolis against all tested microorganisms P

Keywords

Files

Share

How to cite

Statistics