SEM and Energy Dispersive X-Ray Evaluation Of Smear Layer Removal Using Standard And Innovative Irrigation Protocols: A Comparative In Vitro Study

Document Type : Original Article

Authors

1 Department of Endodontics, Faculty Of Dentistry, Fayoum University

2 Department of Endodontics, Faculty Of Dentistry, The British University In Egypt, El Shrouk, Cairo.

Abstract

Aim
Compare efficacy of Dual Rinse HEDP with sodium hypochlorite to sodium hypochlorite in combination with a finishing rinse with 17% EDTA solution to remove the smear layer.

Materials and methods
Sixteen human maxillary single-rooted, single canalled sound premolars were used. Specimens were distributed into 2 groups and labeled according to irrigation protocol used. Each group consisted of 8 specimens: Group 1 50% NaOCl followed by 17% EDTA. Group 2 The Dual Rinse® HEDP mixed with 50% NaOCl. Roots were prepared for SEM appraisal. The smear layer was assessed and scored. Energy-dispersive X-ray analysis was used to analyze the inorganic chemical structure of the treated dentin of the two tested groups. Data were tabulated and statistically analyzed.Kruskal-Wallis test tailed by Mann-Whitney test compared mean scores between irrigant solutions and root regions groups. Chi-square test compared the frequency distribution of scores between different groups.

Results
Smear layer was reduced with The Dual Rinse® HEDP group. Groups exhibited a statistically significant difference in mean scores within apical region and no statistically significant difference within coronal and middle regions. Groups showed a statistically significant difference in frequency distribution of scores within coronal and apical regions. A statistically significant difference in scores frequency existed in between the three root regions within NaOCl+EDTA group While no difference was present in between the different regions within Dual Rinse® HEPD group.
Energy- dispersive X-ray analysis showed lower peaks of calcium in the Dual Rinse® HEDP.

Conclusion
The Dual Rinse® HEDP can be used as one step irrigant.

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