The Effect of Type and Dose of Anti-Inflammatory Drugs on the Viability and Osteogenic Potential of Dental Pulp Stem Cells

Document Type : Original Article

Authors

1 Dentist, Egyptian Ministry of Health And Population, Mansoura, Egypt

2 Lecturer of Oral Biology, Oral Biology Department, Faculty of Dentistry, Mansoura University, Egypt

3 Professor of Oral Biology, Oral Biology Department, Faculty of Dentistry, Mansoura University, Egypt

Abstract

• Objectives
The use of nonsteroidal anti-inflammatory drugs (NSAIDs) after regenerative surgeries may affect the behavior of stem cells used during the treatment. This study investigates the possible effect of NSAIDs on the proliferation and the osteo-differentiation of dental pulp stem cells (DPSCs) in vitro.
• Methods
DPSCs were isolated, cultured and characterized with human antibodies CD90, CD105 and CD45. Cells were cultured for a period of 1, 3 and 5 days to assess the viability using different drug concentrations of acetylsalicylic acid (ASA) 10, 50, and 200 μg/mL., Diclofenac sodium 10-4 and 10-6 M and Meloxicam 0.01, 0.1, and 1 μM .Selected concentration of ASA 10 μg/mL, Diclofenac sodium 10-6 M and Meloxicam 0.1 μM were used during osteo-differentiation of the cells.
• Results
DPSCs viability assay recorded the highest absorption rate on the 1st day with ASA 10 μg/mL and on the 3rd day with Meloxicam 0.01 μM. On day 5 all concentration of ASA and Diclofenac reported the highest absorption rats. The highest values of calcium compounds was expressed with ASA 10 μg/mL on the 7th day using alizarin red satin.
• Conclusion
Using different types of NSAIDs during the postsurgical regimen can affect the viability and osteo-differentiation of DPSCs.

Keywords

Egyptian Dental Journal
Volume 67, Issue 2 - Serial Number 3
April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
April 2021
Pages 1217-1227

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