Evaluation of Antimicrobial Efficacy of Gaseous ozone and Ozonized water against Enterococcus Faecalis Biofilm

Document Type : Original Article

Author

Lecturer, Endodontics Department, Faculty of Dentistry, Karf Elsheikh University, Kafr Elsheikh, Egypt.

Abstract

40 single-canalled permanent teeth were instrumented then inoculated with Enterococcus faecalis and stored in incubator for one week. After biofilm model conformation by SEM, the samples were irrigated with 5% sodium hypochlorite, gaseous ozone (44 μg ml-1) and gaseous ozone with ozonized water (5 μg ml-1). Root canals were sampled before (S1) and immediately after irrigation (S2). The samples were taken by three dry sterile paper points placed to the full WL kept in the canal for 1 min then transferred to tubes containing .05 mL BHI broth solution. After obtaining 1:10 serial dilution from each sample, aliquots were plated out on KF streptococcus agar plates. Then the plates were incubated anaerobically at 37 C for 2 days .The number of bacterial colonies of E. faecalis were counted and expressed as CFUs using a digital colony counter. All groups showed a significant reduction of CFUs (P < 0.001) while having greater antimicrobial reduction was observed on samples of NaOCl irrigation. There was no significant difference between gaseous ozone and gaseous ozone + ozonized water groups. It can be concluded that the gaseous ozone reduced E. faecalis number significantly but was not able to eradicate it completely. While, ozonized water failed to increase the antimicrobial efficacy of gaseous ozone.

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Egyptian Dental Journal
Volume 68, Issue 2 - Serial Number 5
Conservative Dentistry and Endodontics
April 2022
Pages 1781-1788

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