EVALUATION OF THE EFFICACY OF PROPOLIS AS STORAGE MEDIA FOR AVULSED TEETH

Document Type : Original Article

Authors

1 Dentist at Ministry of Health, B.D.S, Faculty of Dentistry, Minia University

2 Professor of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Ain Shams University.

3 Lecturer of Pediatric and Community Dentistry, Faculty of Dentistry, Minia University.

Abstract

ABSTRACT
Background: The best treatment for tooth avulsion is immediate replantation as it maintains the periodontal ligament (PDL) cell viability. If not possible, the tooth must be kept in a suitable storage medium before seeking dental treatment.
Aim of the study: Evaluation of the efficacy of propolis as storage media in maintaining periodontal ligament cell viability for different time periods, in comparison to milk.
Materials and Methods: Immortalized human skin fibroblasts were cultured and stored in the following media: Dulbecco’s modified Eagle’s medium (DMEM), pasteurized low fat content milk and two different concentrations of propolis (10%, and 20%) that were prepared by diluting stock solution of propolis with either Phosphate buffer saline (PBS) or DMEM or milk for time points of 1, 3, 6 and 12 hours. Cell viability was determined using the SRB assay.
Results: Propolis had a significantly higher percentage of viable cells survived in propolis 10%, 20% storage media in comparison to other storage media: milk, DMEM and PBS through different time periods.
Conclusion: Propolis could be a good naturally available storage media for avulsed teeth.

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Egyptian Dental Journal
Volume 68, Issue 3 - Serial Number 1
July (Orthodontics, Pediatric & Preventive Dentistry)
July 2022
Pages 2115-2121

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