Effect of diazepam and green tea on salivary alpha-amylase secretion and structure of the salivary glands in male Albino rats.

Document Type : Original Article

Authors

1 Lecturer of Oral Histology, Faculty of Oral and Dental Medicine, Misr International University (MIU), Cairo, Egypt

2 Lecturer of Oral Pathology, Faculty of Oral and Dental Medicine, Ain-Shams University, Cairo, Egypt

Abstract

Abstract:

Background: Salivary alpha amylase (SAA) is a salivary enzyme that is thought to play a role in response to psychological and physical stress. Diazepam is a sedative drug that most commonly causes xerostomia. The frequent intake of green tea causes the stimulation of salivary secretion thus counteracting the xerostomic effect of several drugs besides its antioxidant ability.
Objective: to describe the effect of diazepam and green tea on SAA secretion and structure of the salivary glands in male Albino rats, to correlate the results with Ki67 tissue expression as a marker for proliferation and to evaluate the condition of the glandular tissue before and after treatment.
Material and Methods: sixty male albino rats were used. Salivary samples were collected after 30 days of treatment to measure SAA. Immunohistochemical study was performed on specimens retrieved from salivary glands to evaluate the expression of Ki67 before and after treatment. The area fraction expressed by Ki67 was calculated.
Results: A decrease in SAA activity was found in rats taking Diazepam alone and in combination with green tea. A decrease in the expression of Ki67 was noticed after the drug intake while a gradual increase occurred after adding green tea.
Conclusion: The study revealed marked decrease in salivary alpha amylase activity with diazepam. Green tea led to further inhibition of amylase activity with restoration of salivary secretion. An increase in the proliferation of acinar cells marked the role of green tea in tissue repair.

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Egyptian Dental Journal
Volume 68, Issue 3 - Serial Number 3
Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
July 2022
Pages 2365-2382

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