Clinical and Histopathologic Evaluation of the Effect of Laser Photobiomodulation And Epicatechin in the Treatment of Radiation Induced Oral Mucositis in Rat Model

Document Type : Original Article

Authors

1 PhD in LASER applications in Dentistry, Oral Medicine, Diagnosis and Periodontology Department, Faculty of Oral and Dental Medicine, Delta University of Science and Technology, Gamasa, Egypt.

2 PhD Oral Medicine and Periodontology, Oral Medicine, Diagnosis and Periodontology Department, Faculty of Oral and Dental Medicine, Delta University of Science and Technology, Gamasa, Egypt

3 PhD Oral Biology, Oral Biology Department, Faculty of Oral and Dental Medicine, Delta University of Science and Technology, Gamasa, Egypt.

Abstract

Aim: This study aimed to determine the preventive and early-stage anticytotoxic effects of Ga–Al–As diode laser photobiomodulation (LPBM) and Epicatechin (green tea extract) (GT), either alone or combined, when used to treat induced oral mucositis (OM) in rats.
Subjects & Methods: Thirty Wistar rats were included; two of them were sacrificed in the beginning (normal mucosa control). Radiotherapy OM was induced a fractionated course of oral radiotherapy at a rate of 2 Gy/min bilaterally twice weekly for two weeks. On day 6, four animals were sacrificed to confirm OM induction. The remaining animals (24 rats) were equally assigned into four groups: Control group (CG), Laser group (LG), green tea extract group (GG) and combined group (L+G). The continued for 5 successive days in the 3-test groups. Animal sacrifice has then occurred on day 11. The mucositis degree (MD) was clinically evaluated, the histopathological assessment of tissue changes was applied using (H&E) and Monoclonal cytochrome-c antibody.
Results: All treated animals showed a statistically significant difference (P-value ≤ 0.05) than those in CG. The lased animals had a better clinical outcome with a faster healing than GT. The best clinical outcome and healing pattern were shown in (L+G).
Conclusion: Ga–Al–As diode laser and Epicatechin produced protection against radiotherapy induced OM through their antioxidant and anti-apoptotic activity effects. Better outcomes were noted in favor of LPBM over GT.

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Egyptian Dental Journal
Volume 68, Issue 4 - Serial Number 3
October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
October 2022
Pages 3247-3259

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