THE HARMONIOUS INHIBITORY EFFECT OF NATURAL DRUG AMYGDALIN (VITAMIN B17) AND CHEMOTHERAPY(CISPLATIN) ON ORAL SQUAMOUS CELL CARCINOMA CELL LINE

Document Type : Original Article

Authors

1 Faculty of dentistry at minia University

2 Professor, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Minia, Egypt

3 Assistant Professor, Oral and Maxillofacial Pathology, Faculty of Oral and Dental Medicine, Minia University, Minia, Egypt.

Abstract

BACKGROUND: Oral squamous cell carcinoma (OSCC) represents the majority of all cancers of the head and neck. In recent times, the development of antitumor drugs has been transformed from cytotoxic drugs to ameliorate the selectivity of the drug, overcoming multidrug-resistant using natural alternatives. Amygdalin is one of the most common non-conventional anti-cancer drugs. Cisplatin has been used for the treatment of numerous human cancers. Recently combination therapy is the treatment of choice for cancer patients for the reason that it may enhance the efficiency of the combined agents and decreases their toxicities by decreasing the dose required for therapeutic benefits.
AIM: This study was organized to investigate the cytotoxic effect of both amygdalin and cisplatin on OSCC, compare between two effects on OSCC, and investigate the synergistic effect on cytotoxicity of both drugs OSCC.
METHODS: The cytotoxic effect was determined using methyl tetrazolium assay, microscopic examination, and statistical analysis (ANOVA).

RESULTS: Our findings showed that the cytotoxic effect of both drugs on HNO97 cells was dose-dependent. Microscopic examination showed that apoptotic criteria became more apparent in cisplatin/amygdalin mix-treated HNO97 group. Our statistical analysis results revealed significant differences in statistical analysis among mean values of NAF of 50 cells in each group, with lower mean values of NAF of mix 48 group (p ˂ 0.0001).

CONCLUSION: Both Amygdalin and cisplatin have anti- cancer effect on HN097 separately and their combination induced harmonious inhibitory effect on HNO97 cells.

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Egyptian Dental Journal
Volume 69, Issue 1 - Serial Number 3
January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)
January 2023
Pages 217-228

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