Comparative Study of the Cytotoxic Effect of Epoxy Resin and Calcium Silicate Based Sealers (An In-Vitro study)

Document Type : Original Article


Lecturer of Endodontics, Endodontic Department, Faculty of Dentistry, October 6 University, Egypt


Objective: The purpose of this study is to compare the cytotoxicity of bioceramic endodontic sealers (Well-Root ST "Vericom in Gangwon-Do, Korea") with epoxy resin-based endodontic sealers (AH + sealer "Dentsply Sirona, Germany").
Methods: In sterile test tubes, the tested sealers were serially diluted twice. In order to evaluate the proper concentration where fibroblast cells would survive, extraction media were diluted many times utilizing MEM-E Medium (Eagle's minimal essential medium). The cytotoxic effect of concentrations used in the present study (0%, 6.25%, 12.5%, 25%, 50%, and 100%) was assessed by MTT essay after 24 hours using a human fibroblast cell line. Statistical analysis was performed by using One Way ANOVA test, followed by Tukey`s Post Hoc test for multiple comparisons.
Results: All concentrations, with the exception of 12.5% and 25%, did not significantly differ between the two groups. Because group I (AH Plus sealer) had a lower proportion of viable cells than group II (Well-Root ST sealer), it had a larger cytotoxic impact. The concentrations that caused a significant difference between the two groups with a P value of 0.001 were 12.5% and 25%.
Conclusion: Evaluation of the calcium silicate-based sealer (Well-Root ST) cytotoxicity showed superior biological behavior and higher cytocompatibility compared to epoxy resin-based endodontic sealer (AH plus).


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