Comparison of Antibacterial Effect of Novel Triple Antibiotic Paste, Conventional Triple Antibiotic Paste and Diode Laser Beam on Root Canals Infected with Enterococcus Faecalis

Document Type : Original Article

Authors

1 Endodontic Department, Minia University، Egypt

2 Microbiology & Immunology Department, Faculty of Pharmacy, Minia University, Egypt Microbiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia, Egypt.

3 Microbiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia, Egypt.

Abstract

Introduction: Local antibiotic administration has been investigated in addition to employing laser disinfection in light of the progress of non-instrumentation endodontic treatment, lesion sterilization, and tissue healing.
Methods: 96 single-rooted teeth were collected. At the CEJ point, their crowns were severed. The root canals were prepared to work up to file F3 ProTaper in length. The smear layer was removed using EDTA 17% and sodium hypochlorite 2.5% for 5 minutes, and composite was then utilized to close the apical foramen. The teeth were sterilised for 15 minutes at 121°C in an autoclave. The adverse control was then represented by 16 samples. The remaining samples spent 21 days submerged in an E. faecalis-containing solution. The samples were devided into 6 groups: group A received an 810 nm Diode laser beam, group B conventional TAP treatment, group C conventional TAP treatment + a diode laser, group D novel TAP treatment, group E novel TAP treatment + a diode laser beam, and group F control. Each group was divided two to test the impact of the intervention after one day and seven days.
Results: The negative control had the largest amount of bacteria (8687.501352.47), followed by laser (670.62221.22), old paste + laser (503.28186.12), old paste + laser (322.89150.85), and new paste + laser (195.6289.51), while new paste (64.0656.90) had the lowest amount.

Conclusion: When compared to utilizing Laser alone, employing a new Triple antibiotic paste has a promising inhibitory impact on biofilm formation and the development of the E. faecalis bacteria.

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Egyptian Dental Journal
Volume 69, Issue 4 - Serial Number 5
Conservative Dentistry and Endodontics
October 2023
Pages 3175-3184

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