Document Type : Original Article
Authors
1
Demonstrator of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Egypt.
2
Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt.
3
Associate Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt
4
Lecturer of oral and maxillofacial pathology, Faculty of Dentistry, Assiut university, Egypt, drabdelrahman12@aun.edu.eg Assistant Professor of oral pathology, collage of dentistry, City University Ajman, Ajman, UAE, a.abdel@cu.ac.ae
5
Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt.
Abstract
Background: Oral cancer has contributed to a tremendous death rate and the total survival rate is expected to be about 50% after 5 years. MicroRNAs are associated with oral carcinogenesis due to their ability to regulate gene expression. To limit tumor growth, miR-145 influences signaling pathways by targeting tumor-specific genes.
Objective: This research was conducted to investigate the potential impact of miR-145 on the human oral squamous cell carcinoma cell line (OECM-1), and Human Oral Fibroblast cell line (HOrF) as negative control cells. Also, to investigate its impact on the expression of C-Myc and Caspase-3 genes.
Material and Methods: In this study, there were four groups. Group I: untreated OECM-1, Group II: treated OECM-1, Group III: Untreated HOrF, and Group IV: treated HOrF. MiR-145 inhibitor was transfected into cell lines. Methyl Thiazole Tetrazolium assay was used to analyze the vitality and oralthe cell proliferation rate of the cell lines. Furthermore, the expression of C-Myc, and Caspase-3 was assessed in OECM-1 and HOrF cell lines using SYBER green-based qPCR.
Results: The findings demonstrated a significant increase in the cell viability and the proliferation rate after transfection of OECM-1 cells with miR-145 inhibitor. In addition, miR-145 inhibitor transfected cells enhanced C-Myc gene expression and decreased Caspase-3 gene expression OECM-1.
Conclusion: Suppression of miR-145 caused a higher proliferation rate in Human Oral Squamous Cell Carcinoma (OECM-1) by increasing C-Myc gene expression and downregulating the level of Caspase-3 gene.
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