Effect of Incorporating Three Natural Antimicrobial Agents into Tissue Conditioner in Inhibiting the Growth of C. Albicans and S. Mutans: An In-Vitro Study

Document Type : Original Article

Authors

1 Associate Professor of Dental Materials Science, Biomaterials Department, Faculty of Oral and Dental Medicine, Egyptian Russian University; ERU.

2 Associate Professor of Dental Materials Science, Biomaterials Department, Faculty of Dentistry, Cairo University.

Abstract

Background: Incorporation of natural antimicrobial agents into tissue conditioners might inhibit microbial colonization and prevent complications such as denture stomatitis.
Aim of the study: The present study compared antimicrobial properties of tissue conditioner (TC) incorporated with two (5% and 10%) concentrations of pumpkin (PK), rosemary (Rm), or propolis (Pp) extract powders at different storage times (3 and 7 days) against Candida albicans (C. albicans) and Streptococcus mutans (S. mutans).
Materials and Methods: Pilot study was performed to determine minimum inhibitory concentration (MIC) of Pk and Rm against C. albicans and S. mutans using broth-microdilution method. TC disc-shaped samples (n=3/group) mixed with (5% and 10%) concentrations of either Pk, Rm or Pp powders, positive and negative control groups were prepared. Agar-diffusion test was performed and zones of inhibition were measured in millimeters after 3 and 7-days. Data were statistically analyzed.

Results: Maximum inhibition zone against C. albicans was seen in 5% and 10% Pp after 3 & 7 days, which was greater than effect of +ve control group at same periods. For S. mutans, maximum inhibition zones were seen with +ve control group at both time intervals, followed by 10 Rm and 10 Pk extracts after only 3 days. Propolis extract produced larger inhibition zones than Rm and Pk after 7 days at both tested concentrations.
Conclusion: Propolis extract powder incorporated into TC exhibited considerable antimicrobial efficiency against both C. albicans and S. mutans. Incorporation of natural antimicrobial agents in TC can be used as effective alternative to topical synthetic agents.

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Egyptian Dental Journal
Volume 71, Issue 2 - Serial Number 4
Fixed Prosthodontics, Removable Prosthodontics and Dental Materials
April 2025
Pages 1593-1604

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