Document Type : Original Article
Authors
1
Department of Oral Biology, Faculty of Dentistry, Cairo University, Cairo, Egypt Department of Oral Biology, Faculty of Dentistry, Suez University, Suez, Egyp
2
Department of Oral Biology, Faculty of Dentistry, Cairo University, Cairo, Egypt
3
Department of Oral Biology, Faculty of Dentistry, Suez University, Suez, Egyp
4
Department of Medical Histology, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Objectives: This study aimed to characterize the histological and histomorphometric changes in inflamed dental follicle (DF) compared to normal DF using routine and specialized staining techniques.
Materials and methods: Twenty human DF tissue specimens (ten normal, ten inflamed) were sectioned. The samples were stained using Hematoxylin and Eosin (H&E) and picrosirius red to assess collagen fiber distribution. Histological features, including epithelial lining, connective tissue cellularity, collagen fiber density and organization, and interstitial edema, were evaluated. Quantitative analysis of collagen fiber area percentage, interstitial edema area percentage, and inflammatory cell number were performed; to ascertain statistical significance, the data underwent analysis of variance (ANOVA), and significant outcomes were further explored via post hoc tests.
Results: H&E staining of inflamed DF revealed increased cellularity with inflammatory cell infiltration and interstitial edema compared to normal DF, which showed a well-organized stratified epithelium and delicate connective tissue. Picrosirius red staining demonstrated a significantly denser and more disorganized collagen network in inflamed DF compared to the loosely arranged, delicate fibers in normal DF (P < 0.001). Quantitative analysis confirmed that the level of increase was statistically significant (P < 0.001) in collagen fiber area percentage, interstitial edema area percentage, and inflammatory cell number in the inflamed group.
Conclusion: Inflammation in the DF is associated with significant histological alterations, including increased inflammatory cell infiltration, interstitial edema, and increased collagen density and disorganization. These findings underscore the dynamic tissue response within the DF during inflammation and the possibility of odontogenic cyst formation
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