Efficacy of Asiaticoside as an Intracanal Medicament on Bacterial Load Reduction in Primary Infected Root Canals: A Randomized Clinical Trial

Document Type : Original Article

Authors

1 Master student, Department of Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt

2 Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt

3 Department of Conservative Dentistry and Endodontics, Faculty of Dentistry, Mansoura University, Mansoura, Egypt

Abstract

Abstract
Objective: This research aimed to clinically study the effectiveness of asiaticoside as intracanal medication compared to the commonly used medicaments (Ledermix and Ca(OH)2 ) in the reduction load of bacteria in necrotic teeth.
Materials and Methods: This study comprised 36 patients with single-rooted, single-canal necrotic teeth who suffered from chronic periapical periodontitis.
Before any clinical procedures, aseptic control measures were implemented. Access cavities were made, and pre-disinfection samples (S1) were obtained immediately afterward, before root canal cleaning and shaping.
The subjects were divided into three groups depending on the nature of the intracanal medicament utilized (Ca (OH)2), Ledermix, and asiaticoside. A second set of samples (S2) was collected one week after administering the intracanal medicaments. Both samples were transported in blood agar dishes for anaerobic culturing in the microbiology lab. The evolution of bacterial growth was measured as colony-forming units (CFUs) and calculated manually counting technique. The antibacterial effectiveness of intracanal medicaments was assessed by calculating the percentage reduction in bacterial colonies from S1 to S2.
Results: All evaluated intracanal medicaments showed a significant reduction in CFUs from S1 to S2 (P<0.05). Asiaticoside and Ledermix had significantly lower CFUs than Ca(OH)2 (P < 0.05), however, there was no significant difference between them (P > 0.05).
Conclusion: Asiaticoside might be considered a potential root canal medicament due to its equivalent anti-microbial impact with Ledermix and greater efficacy than Ca(OH)2 medicament.

Keywords

Main Subjects

Egyptian Dental Journal
Volume 71, Issue 3 - Serial Number 5
Conservative Dentistry and Endodontics
July 2025
Pages 2621-2631

Files

Share

How to cite

Statistics