Comparative Evaluation of Enamel Superficial Microstructure and Microhardness following Application of Coral Calcium Nano Silver MI Paste Plus Tooth Paste on Management of White Spot Lesion (An In Vitro Study)

Document Type : Original Article

Authors

1 Master Degree Candidate, Department of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Cairo University, Cairo, Egypt

2 Professor of Pediatric Dentistry and Dental Public Health, Faculty of Dentistry, Cairo University, Cairo, Egypt

Abstract

Aim of the study: To compare remineralizing potential of Coral Calcium Nano Silver toothpaste versus MI paste plus toothpaste on primary molar white spot lesions, evaluating enamel superficial microstructure and microhardness.
Methods: Sixteen extracted human primary molar teeth were randomized into two groups (n=8), mounted in acrylic resin, and a 2×2 mm buccal enamel window was created. Artificial white spot lesions were induced via 72-hour demineralization (pH 4.6). Subsequently, Samples underwent a 14-day remineralization protocol with daily 3-minute agent application and artificial saliva storage. Surface morphology evaluation was done using Scanning Electron Microscopy (SEM) and surface microhardness by a Vickers Microhardness Tester at baseline, post-demineralization, and post-remineralization. Data were analyzed using a two-way mixed model ANOVA with Tukey’s post-hoc comparisons (p<0.05).
Results: SEM analysis revealed that both groups exhibited favorable morphological alterations on the enamel surface post-remineralization. Microhardness values significantly varied across intervals (p<0.001), with post-remineralization being highest and post-demineralization lowest; baseline and remineralization values significantly exceeded demineralization. Baseline and remineralization values were significantly higher than demineralization values. While the Coral group showed numerically higher microhardness, no statistically significant difference was observed between the two materials. Only time demonstrated a significant effect on microhardness, while the effect of material and its interaction with time were not statistically significant.
Conclusion: Both Coral Calcium Nano Silver and MI Paste Plus Toothpaste are equally effective in remineralizing the enamel of the primary teeth. Although, Coral Calcium Nano Silver showed a higher microhardness, no significant differences were observed between the two toothpastes.

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Egyptian Dental Journal
Volume 71, Issue 4 - Serial Number 1
October (Orthodontics, Pediatric & Preventive Dentistry)
October 2025
Pages 2885-2898

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