Document Type : Original Article
Authors
1
Lecturer of Oral Medicine and Oral Diagnosis- Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Menoufia University, Egypt.
2
Professor of Medical Biochemistry, Faculty of Medicine, Cairo University, Egypt.
3
Lecturer of Oral biology, Faculty of Oral and Dental Medicine, Egyptian Russian University
4
Lecturer of Oral and Maxillofacial Surgery, Faculty of Dentistry, Menoufia University, Egypt.
5
Lecturer of Oral Medicine and Oral Diagnosis- Oral Medicine, Periodontology, Oral Diagnosis and Radiology Department, Faculty of Dentistry, Modern University For Technology and Information, Egypt.
Abstract
Oral squamous cell carcinoma (OSCC) is one of the most common tumors in the head and neck area. Recent research suggests that the increased expression of the long non-coding RNA (lncRNA) HOTAIR correlates with the progression of many cancer types.
The aim of this study: The objective of this research was to ascertain if levels of long non-coding RNA HOTAIR in saliva are advantageous and correspond among the oral squamous cell cancer (OSCC) group, the oral potentially malignant lesions (OPMLs) group, and the control group.
Materials & Methods: A total of 45 participants (n=15) were evenly allocated into three distinct groups. Group A, diagnosed with OSCC; Group B, diagnosed with OPMLs; and Group C, the control group. Saliva samples were collected from participants through unstimulated spitting and subsequently preserved in sterile vials. The extraction of LncRNA HOTAIR from saliva was conducted, followed by preparation for genetic analysis utilizing Reverse Transcription (RT) and real-time Quantitative PCR (qPCR).
Results: No statistically significant variations were found between the groups concerning gender; nevertheless, highly statistically significant disparities were noted between the median ages of the control group and those of the premalignant and OSCC groups. Concerning HOTAIR expression, a significantly significant disparity was seen between the OSCC and control groups, however no significant difference was noted between the OPMLs and control groups.
Conclusions: The expression of HOTAIR is linked to OSCC and may serve as a crucial target in its evolution. Nonetheless, HOTAIR is not implicated in the tumor progression of OPMLs.
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