Document Type : Original Article
Authors
1
MSc Student, Department of Endodontics, Faculty of Dentistry, Minia University, El-Minia, Egypt
2
Professor, Microbiology & Immunology Department, Faculty of Pharmacy, Minia University, Egypt Microbiology and Immunology Department, Faculty of Pharmacy, Deraya University, Minia, Egypt.
3
Assistant Professor Department of Endodontics, Faculty of Dentistry, Minia University, El-Minia, Egypt
4
Microbiology and Immunology , University of Deraya , Minia , Egypt
Abstract
Introduction: Chemo-mechanical preparation plays a crucial role in cleaning the root canal by eliminating microorganisms, pulp tissue, and the smear layer. Traditional methods alone are insufficient; therefore, enhancing endodontic irrigants with nanoparticles and activating them ultrasonically is recommended to boost their antimicrobial effectiveness.
Methods: Eighty mature, single-rooted human permanent teeth were decoronated, processed, sterilized, and then inoculated with Enterococcus faecalis. The specimens were randomly assigned to four groups based on the irrigant used: Group 1 was treated with magnesium oxide (MgO) nanoparticles, Group 2 with 5.25% sodium hypochlorite (NaOCl), Group 3 with a combination of MgO and 5.25% NaOCl, and Group 4 with saline. Each group was split evenly into two subgroups, labeled A and B. Subgroup A received passive irrigation, whereas in subgroup B, the irrigant was activated using ultrasonic agitation for 5 minutes at a frequency of 25 Hz. The number of bacterial colonies was counted to measure antimicrobial effectiveness.
Results: The negative control had the largest amount of bacteria , followed by group irrigated by NaOCL 5.25 , followed by group irrigated by MgO, while group irrigated by Mixed solution had the lowest amount.
Conclusion: Magnesium oxide nanoparticles demonstrate enhanced antibacterial properties, which are further amplified when combined with ultrasonic activation.
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