Assessment of Antimicrobial characteristics of novel enamel treatment protocol using silver gold nanoparticles with orthodontic brackets for caries prevention: In Vitro Study

Document Type : Original Article

Authors

1 Lecturer of Orthodontics, Faculty of Dentistry, Ahram Canadian University

2 Assitant professor of Conservative Dentistry, Faculty of Dentistry, Cairo University

3 Professor of Conservative Dentistry, Faculty of Dentistry, Cairo University

Abstract

Objectives: The aim of this study is to evaluate the antimicrobial activity of novel enamel pretreatment protocol using silver-gold nanoparticles to enamel bonded orthodontic brackets on Streptococcus mutans and lactobacillus strains.
Methodology: Twenty sound upper anterior teeth were used. They were equally divided into two groups. In Group A: The NanoCare gold was applied after enamel etching, followed by application of the bonding agent then the composite adhesive. As for Group B (control group): the etching procedure was followed by applying the bonding agent directly then by the composite adhesive. All teeth were cut off from the cured acrylic resin below the crown portion by 2 mm. Bacterial testing was carried out using the agar well diffusion method. The inhibition zone was measured after 24 h, 48 h and 72 h. TEM assessment of the mode of action was performed data were collected and statistically analyzed. The significance level was set at p<0.05 within all tests.
Results: Results showed that inhibition zones formed with the NanoCare gold group were significantly higher than those of the control group. TEM results revealed rupture of the bacterial cell membrane and penetration of nanoparticles inside the cells causing their death.
Conclusions: the treatment protocol using Silver-Gold nanoparticles was successful in inhibiting several types of microbial strains such as S. mutans and Lactobacilli around orthodontic brackets.
Clinical significance: Enamel treatment with silver-gold nanoparticles prior to the cementation of orthodontic brackets can decrease the chance of the demineralization during treatment than uncoated brackets.

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Egyptian Dental Journal
Volume 71, Issue 4 - Serial Number 5
Conservative Dentistry and Endodontics
October 2025
Pages 3469-3482

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