HISTOPATHOLOGICAL EVALUATION OF EFFECT OF LASER VERSUS FOSFOMYSINE IN TREATMENT OF OSTEOMYELITIS INDUCED BY METHICILLIN-RESISTANT

Introduction: Fosfomycin has been shown highly effective in the treatment of experimental MRSA osteomyelitis. Laser therapy appeared to be a promising alternative technique to pharmacological agents in the treatment of osteomyelitis. Consequently, this study aimed to determine the effects of fosomycin and laser in the treatment of induced osteomylitis by MRSA in rabbits.
Material and methods: Localized osteomyelitis was induced in rabbits by percutaneous injection of 0.1 ml of 108 cfu of MRSA clinical isolate into the intramedullary cavity of tibia. After two weeks, tibial osteomyelitis was confirmed radiographically. Rabbits were divided into three groups (each group contained 10 rabbits): untreated controls group, group treated with fosfomycin 75 mg/kg subcutaneously every 24 h and group treated with Diode laser 940 nm, three times weekly. After 14 days of treatment, rabbits were left untreated for 24 hours then euthanized and the tibias harvested. evaluation of treatment was done by measurement of bone density radiologically, Histopathological examination and each tibia were cultured for bacterial counts determination
Results: Bacterial cultures from bones were positive for MRSA in 8 out of 10 (80%) rabbits in the control group, in 9 out of 10 (90%) rabbits in the fosfomycin and laser treated group . Laser group showed the highest bone density among the studied groups. No significant difference was found in the bone cultures and bone density between the three groups; P value 0.302 and 0.157 respectively