Evaluation of Naturally Derived Hydroxyapatite Tissue Engineering Scaffold Coated With Chitosan-Carbon Nanotubes Composite

Document Type : Original Article

Authors

1 Dental Biomaterials Dept., Faculty of Oral and Dental Medicine, Zagazig University

2 Refractories, Ceramics and Building Materials Department, National Research Centre

3 Medical Experimental Research Centre (MERC), Faculty of Medicine, Mansoura University

4 Experimental Solid State Physics Dept., Faculty of Science, Mansoura University, Egypt

Abstract

Objective; This study aimed to valuate the effect of f-CNTs (functionalized carbon nanotubes) and chitosan (CS) composite on the mechanical properties and cell biocompatibility of carbonated HAp (hydroxyapatite) derived from cuttlefish bone.
Methods; The microstructure characterization of carbonated HAp after hydrothermal treatment showed complete transformation into highly crystalline structure. The prepared CS/f-CNTs composite was introduced into HAp specimens under vacuum. A compressive load was applied till failure using a universal testing machine to evaluate compressive strength and elastic modulus of aragonite (CaCO3) and treated HAp with CS/f-CNTs composite. The proliferating number and percentage of MSCs (mesenchymal stem cells) in direct contact with tested materials were used as indication of cytotoxic effect after 24, 36 and 72 hours in addtion alkaline phosphatase activity (ALP) after 12 days was used as indication of osteogenic effect.
Results; The HAp group with deposition of CS/f-CNTs composite showed a statistically significant higher compressive strength (0.82 ± 0.17 MPa) and elastic modulus (11.1 ± 1.06 MPa) and a statistically significant lower value for cell viability percentage (225,590 ± 8030.503, 32.95%) after 72 hours and ALP activity (85.51 ± 5.67 μU/μg protein) after 12 days of incubation.
Conclusions; Carbonated HAp scaffolds reinforced with CS/f-CNTs composite can be used in low stress bonny defects.

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