EVALUATION OF PHYTOESTROGENS OF SOYBEAN IN FORM OF SEMI SOLID PHARMACEUTICAL PASTE AS A GRAFT MATERIAL IN BONY DEFECTS

Purpose: to evaluate the effectiveness of phytoestrogens of Soybeans in form of semi solid pharmaceutical paste as a graft material in bony defects.
Material and Methods: Twelve bony defects were created in the parietal bones of nine New Zealand white rabbits. Two groups were divided (Six defects each). One gm of the purified active fraction obtained from seeds of Glycine max L. (Soybean) Family Leguminosae, rich in isoflavones was prepared in form of a semi solid pharmaceutical paste and have been used as graft material for defects of study group. While other defects in control group were left empty. Defects and surrounding tissue were decalcified and routinely processed for Haematoxylin and Eosin staining. The area percent of newly formed bone was estimated using an image analyzer computer system. Comparison of the results of both groups was carried out. ANOVA and T-Test were used for statistical analysis.
Results: Regularly arranged, osteoid newly formed bone almost filled the surgical defect in study group. Scarce bone trabeculae and wide fibrovascular marrow spaces were observed in the control group. This difference was statistically significant (P<0.0001).
Conclusion: The results of this study suggest that phytoestrogens obtained from Soybeans seeds in form of semi solid pharmaceutical paste is an effective alternative graft material. Further studies are needed on extended time intervals.