Antibacterial and Mechanical Assays of Resin Modified Glass Ionomer Containing Propolis Extract

Document Type : Original Article

Authors

1 Associate Professor of Pedodontics, Faculty of Dentistry, Kafrelsheikh University, Egypt

2 Associate Professor of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt

Abstract

The antibacterial efficacy of restorative materials had an important role in preventing the recurrent caries. The objective of this study was to evaluate in-vitro the antibacterial and mechanical assays of Vitremer containing ethanolic extract of propolis (EEP). Materials and methods: I-Antibacterial Assay: The standard strain of Streptococcus mutans and Lactobacillus acidophilus were used for determination of minimum inhibitory concentration (MIC) of EEP/Vitremer. II-Mechanical Assay: A-Shear bond strength (SBS): sixty half-crowns of non-carious extracted 2nd primary molars were placed at standard moulds containing Teflon disc that had 4mm x 3mm central hole and divided into 4 groups (n=15) according to the different EEP concentrations. Group I: 10%, group II: 25%, group III: 50% EEP-Vitremer mixture, and group IV (control): 0% EEP/Vitremer and SBS was assessed using Instron machine. B-Microhardness: Sixty standard disc-shaped specimens were prepared from mixture 0%, 10%, 25%, and 50%, n=15 and nanoindentation value was recorded. Data were analyzed using one-way ANOVA and post-hoc test. Results: Only MIC of 10%, 25%, and 50% mixture showed growth inhibition against S. mutans, compared to 25% and 50% against L. acidophilus. SBS showed that 0% EEP recorded the highest value followed by10% mixture but the difference was not significant (p>0.05), while 25% and 50% reported the lowest values and the differences were significant (p< 0.05). 25% and 50% mixtures recorded the highest significant microhardness (p<0.05). 0% EEP and 10% mixtures displayed no significant differences between them (P>0.05). Conclusions: 25% EEP-Vitremer mixture was the most suitable concentration as it exhibits positive significant antibacterial and mechanical assays.
Keywords: Propolis extract, fluoride-releasing restoratives, inhibition of recurrent caries, antibacterial and mechanical assays.