Antibacterial Activity of Three Tooth-Colored Restorative Materials against Streptococcus mutans : An in Vitro Study

Document Type : Original Article

Authors

1 Associate Professor- Pediatric Dentistry and Dental Public Health- Faculty of Dentistry- Cairo University

2 Lecturer of Immunology and Microbiology- Immunology and Microbiology Department – Faculty of Medicine- Cairo University.

Abstract

Background: Antibacterial activity of restorative materials had an important role in preventing
caries recurrence.
Aim of the study: to evaluate the antibacterial effect of three tooth-colored restorative materials.
Materials and Methods: Three commercially available tooth-colored restorative materials,
GC Fuji (Group I), Riva self-cure (Group II) and Versa Comp (Group III) were evaluated. The test
samples of restorative materials were manipulated in accordance with manufacturer’s instructions.
Eppendorf tubes were filled with constant measured amount of the three tested restorative materials;
a conical cavity was created in the center of each material after curing. Bacterial suspension was
prepared from fresh overnight growth of Streptococcus mutans on blood agar. 50 μL of bacterial
suspension was placed in the conical cavity and around the restorative material inside each
eppendorf tube and then were incubated. At the end of incubation period, eppendorf tubes were
removed from candle jar and viable bacterial count was estimated.
Results: It has been found that at day1, day 2 as well as day 3, there was decrease in bacterial
count but, there was no statistically significant difference between the three groups. On studying
each group separately, group I there was a statistically significant decrease in bacterial count at day
2 and group II and III showed statistically significant decrease from day 2 to day 3.
Conclusions: Fuji LC was more effective against Streptococcus mutans, than Riva self-cure
and Versa comp, but with no significant difference.

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