Transmission electron microscopy and immunohistoc hemical studies on the effect of platelet rich fibrin at the bone- metallic interface in the mandible

Background: A dental implant is an effective method for restoring edentulous dentition. Many
biological materials were used to coat implant surfaces to enhance osseointegration. Growth factors
like bone morphogenic proteins (BMP) were used for this purpose. Platelet rich fibrin (PRF ) is a
new approach on bone regeneration through the synergistic effect of many growth factors derived
from platelets.
Purpose: The purpose of this study was the evaluation of the effect of platelet rich fibrin (PRF )
and transforming growth factor (TGF β) on the bone- metallic interface in dogs. Also, to determine
the relationship between titanium implants and bone cells by the electron microscopy.
Methods & materials: Six healthy adult male dogs included in this study. The dogs were
divided randomly into two groups. The dog’s mandibles were used. The third premolar was
extracted bilaterally and the right side used as a control while the left used as experimental side. PRF
was prepared and the immediate implant was inserted. Ultrastructure and immunohistochemical
examination of dogs were evaluated.
Results: The results demonstrated that the PRF and TGF β enhance the osseointegration in the
experimental group when compared with control group. During study of bone regeneration more
advanced intervals were required for bone maturation.
Conclusions: The PRF accelerates early bone regeneration and there is a tight regulatory
relationship between the TGF β and the rapidity of bone formation.