Investigation the Cytoto xicity and Apopto sis of annonaceae nanopartic les and 5-FU encapsulated chito san nanopartic les against Tongue Squamous Cell carcinoma Cell Lines (SSC-25)

Document Type : Original Article

Authors

1 Lecturers, Oral Pathology Department, Faculty of Oral and Dental Medicine, Cairo University

2 Lecturers, Oral Surgery Department, Faculty of Oral Surgey, Misr University for Science and Technology.

Abstract

Objective: cancer result from abnormal genetic makeup of a cell resulting in continuous cell
division with often production of metastasis throughout the body. Apoptosis is very effective in
killing cancer cells. We performed this study to investigate the effect of annonaceae and 5FU drug
nanoparticles against scc-25 cell lines human tongue squamous cell carcinoma by studying their
effect on cell cycle progression and apoptosis
Methods: Cell Line cells were obtained from American Type Culture Collection , cells were
cultured using DMEM (Invitrogen/Life Technologies). Annonaceae nanoparticles and 5FU drug
were used. The effect of the tested materials were investigated by MTTassay, cell cycle analysis &
flow cytometry.
Results: the cancer cells treated by annonaceae nanoparticles revealed obvious cytotoxicity at
iC50 7 μg/ml, in addition it stimulated the upregulation of annexin-v leading to apoptosis of cancer
cells and growth arrest at G1 phase with prominent late apoptosis analyzed by cell cycle analysis
& flow cytometry.
Conclusion: Our findings demonstrate that annonaceae nanoparticle might be capable of
inducing cancer cell death against SCC-25 cell lines by cell cycle arrest and induction of apoptosis.

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