Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line

Document Type : Original Article

Authors

1 Lecturer, Oral Pathology Department, Faculty of Oral and Dental Medicine, Cairo University

2 Lecturer, Oral surgery Department, Faculty of Oral surgey, Misr University for science and technology

3 Lecturer, Oral surgery Department, Faculty of Oral surgey, Misr University for science and technology.

Abstract

Objective: Nanoparticle delivery systems can be used to target anticancer drugs to tumor
tissues. Beet root appears to be a powerful dietary source of antioxidant agent with anti-cancer
effect due to ability to scavenge free radicals. Application of silica-nanoparticles (SiNP) in cancer
treatment are promising, with increased data suggesting anti-proliferative effects in cancer cells.
The aim of our study was to compare the cytotoxic effect of the beet root NP, silica-beet NP with
5fu (flourarcil) NP , a chemotherapeutic agent against tongue carcinoma cell line scc-090.
Methods: Cell Line cells were obtained from American Type Culture Collection , cells were
cultured using DMEM (Invitrogen/Life Technologies). Beet root nanoparticles Si-beet NP and 5FU
drug NP were used. The effect of the tested materials were investigated by MTTassay and DNA
fragmentation (DPA method).
Results: The MTT cytotoxicity assay revealed that beat root (nano) and Si-beat roots (nano)
have obbvious cytotoxicity on SS C-090 cancer cell lines at low significant IC50 value as 0.73μg/
ml and 2.94 μg/ml respectively. While 5FU drug revealed obvious cytotoxicity on SSC-090 cancer
cell lines at higher value IC50 8.83 ug/ml. these results denote that low doses of beat root (nano)
may exert remarkable cell death on tongue cancer cells line scc-090.
Conclusion: Our findings demonstrate that beet root NP could be capable of inducing cancer
cell death with prominent apoptosis at very low ic50 value 0.73μg/ml against oral tongue carcinoma
SCC-090 cell lines.
 

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