Aim: To compare the efficacy of chicken egg shell solution powder (CESP) and amorphous calcium phosphate (ACP) on the remineralization of enamel subsurface lesions. Materials and Methods: Twenty bovine central incisor teeth were used in this study. The microhardness and the minerals content of all specimens were initially assessed using Vickers hardness tester and EDXA respectively (positive control group).The specimens were artificially demineralized and then reassessed directly after demineralization (negative control group). All demineralized specimens were randomly classified into 2 equal groups,group I :treated with Chicken eggshell powder solution (10 specimens), while, groupII: treated with ACP (10 specimens). The remineralizing agent were applied twice daily for 15 min each for seven successive days. Finally all specimens were reassessed for minerals content (Ca and PO4 weight %) and surface microhardness. The data were statistically analyzed using repeated measures analysis of variance (ANOVA ), the significance level was set at P ≤ 0.05. Results: Microhardness was significantly decreased in all specimens after demineralization and then it was significantly increased after exposure to therapeutic solutions. There was a significant increase (P<0.001) in both Calcium and Phosphorus levels after remineralization using both agents, as detected by EDAX. However, there was no significant difference between the two remineralized groups. Conclusions: Within the limitations of this in vitro study, it can be concluded that both remineralizing agents were similarly able to increase the microhardness and tooth remineralization. However, being natural products, CESP can be considered as an optimal alternative to the commercial ones.
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