DEGREE OF DENTINAL TUBULES OCCLUSION USING DIFFERENT OCCLUDING AND SEALING TECHNOLOGIES

Aim: the purpose of this study is to evaluate the effect of using nanohydroxyapatite, self assembly peptides, resin modified glass ionomer (RMGI) technologies on the degree of dentinal tubules occlusion in comparison to conventional applied sodium fluoride varnish.
Methods: Forty flat dentin specimens were prepared from sound human premolars. The speci­mens were immersed in 6% citric acid solution for 3min. Each dentin specimen was divided into two equal halves. One half acted as a control and the other received the treatment. The specimens were then divided into four equal groups according to desensitizing agent applied (n=10): GI: The nanohydroxyapatite based (Desensibilize NanoP, Brazil), GII: The self assemply peptide based (Curodont D’SenZ, Schweiz), GIII: RMGI based (VanishTMXT, USA), GIV: Sodium fluoride based (Durasheild, USA). The extent of dentinal tubules occlusion was assessed using environmental field emission scanning electron microscopy (EFESEM). EFESEM photomicrographs were cap­tured from each half (Control and treated) after; 24 hours, 7 days and exposure to acidic challenge. EFESEM photomicrographs were imported into Image analysis software (NIH, USA) and percent­age of dentinal tubules occluded areas was calculated. Data was analyzed using ANOVA test.
Results: The two ways ANOVA revealed that both desensitizing agents and time intervals and their interaction have a statistical significant effect on the degree of dentinal tubules occlusion. The groups after both 24 hours were ranked: GIII>GI=GII>GIV, after 7 days: GIII = GII > GI> GIV, and after the exposure to acidic challenge: GIII>GI>GII>GIV
Conclusion: The RMGI sealing technology showed a more promising effect on the degree of dentinal tubules occlusion compared to the innovative occluding technologies, based on the self assembly peptides, or conventional sodium fluoride varnish
Clinical relevance: The use of RMGI sealing technology can show immediate relief of dentin hypersensitivity owing to its immediate and lasting effect. Meanwhile the innovative occluding technologies based on the nanohydroxyapatite and self assembly peptides, need sometime to show a more profound effect. Nevertheless they are not recommended to patients with high citrus acid products consumption.