ANTIBACTERIAL EFFECT OF FOUR IRRIGATING SOLUTIONS COMPARED TO THAT OF SODIUM HYPOCHLORITE (A COMPARATIVE IN-VITRO STUDY)

Document Type : Original Article

Authors

1 Research Postgraduate Student

2 Professor of Endodontics, Faculty of Oral and Dental Medicine, Cairo University

3 Lecturer of microbiology, Faculty of Dentistry, October 6 University

Abstract

Aim : The aim of the present study was to evaluate the antimicrobial activity of four irrigating solutions against Enterococcus faecalis compared with sodium hypochlorite 5.25% namely: propolis extract, QMix, Chloroxylenol and CHX-Plus
Materials and methods: Enterococcus faecalis (batch no. 10541) thawed and incubated under aerobic condition. The harvested bacterial colonies are then spectrophotometrically calibrated to 5.9 x 104 (CFU/mL-1). Fifty specimens instrumented conventionally, reaching master apical file size #50. Twenty μL of the bacterial culture transferred to the canal lumen using sterile micropipette. All specimens were then incubated for 48 hours at 37 ˚C.
 
Results : Intracanal sample collection by paper points showed an insignificant difference between different irrigation solutions tested and positive control Group at p=0.001. Group 1 showed a median of 8.9*104(CFU/ml), where group 2, 3, 4 and positive control groups showed mean values of 2.2*104, 7.3*104, 8*104  and 3*104  (CFU/ml) respectively. Negative control group showed the highest significant values for colony forming units (118.4*104CFU/ml).
 
Conclusion : All groups except the saline produce of significant reduction of bacteria. Although QMix, NaOCl and chlorhexidine plus are capable of reducing bacterial count their effect is statistically insignificant

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