THE EFFECT OF OXIDATIVE STRESS ON MITOCHONDRIAL ULTRASTRUCTURE AND IMMUNOEXPRESSION OF ERK-1 AND HIF-1 ALPHA IN ORAL EPITHELIAL DYSPLASIA INDUCED IN EXPERIMENTAL ANIMALS

Document Type : Original Article

Authors

1 Lecturer of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University

2 Assistant Professor of Oral Pathology, Faculty of Oral and Dental Medicine, Cairo University

3 Assistant Professor of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University

Abstract

Background: Oxidative stress and accumulation of reactive oxygen species may play a role in the pathogenesis of cancer. They were reported in precancerous and cancerous cells.
 
Methods: An animal model of carcinogenesis was used to detect the effect of oxidative stress on mitochondrial ultrastructure by transmission electron microscope and the levels of extracellular signal- regulated kinase-1(ERK-1) and hypoxia inducible factor-1 alpha (HIF-1α) by immunohistochemistry in mild and severe oral epithelial dysplasia (OED) induced in tongues of experimental mice.
 
Results: We identified degeneration and enlargement of mitochondria in association with up- regulation of both ERK-1 and HIF-1α from normal control to mild OED and reached their highest values in severe OED.
 
Conclusions: Degeneration and enlargement of mitochondria in association with up-regulation of both ERK-1 and HIF-1α can be indicative features of dysplastic changes and may act as early markers for malignancy.
 

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