HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY OF CHROMIUM TOXICITY IN RAT SUBMANDIBULAR SALIVARY GLANDS

Document Type : Original Article

Authors

1 Associate Professor of Oral Biology Department, Faculty of Dentistry, Alexandria University

2 Lecturer of Oral Biology Department, Faculty of Dentistry, Alexandria University

Abstract

24 male albino rats (200-220 mg body weight) were utilized in the current study. They were divided into three groups. The first group was control group (6 rats) received drinking tap water containing permissible correlation of hexavalent chromium Cr (VI) 50-100 Mg/L for 4 months. The drinking tap water with added 450 mg/L of hexavalent chromium as chromium trioxide (Cr2O3) for 4 months. Study group (II) (9 rats) received same drinking water as study group (I) with administrated
2.5 mg vitamin E oil daily along four months. Samples from submandibular salivary glands were prepared for histological examination with (H&E stains) and immunohistological investigation of ki-67 proliferation matrix. Group (I) animals revealed histopathological degenerative changes in submandibular salivary gland acini. The blood vessels were dilated and engorged. The connective tissue stroma revealed increase in thickness. The immunoexpression of Ki-67 in the nuclei of the cells was less than 5% of the same group, where the ducts of the acini showed increased percent of Ki-67 expression. Group (II) received vitamin E in addition to Cr (IV), showed some recovery to normal structure of the submandibular salivary glands. This might states the protective effect of Vitamin (E) as an antioxidant against cytotoxic effect of hexavalent chromium.

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