In-vitro evaluation of the effects of sterilization procedures on the antibacterial characteristics of zinc oxide nanoparticles coated orthodontic mini screws.

Document Type : Original Article

Authors

1 Post graduate student, Orthodontic Department, Faculty of Dentistry, Minia University

2 Professor of Material Science, Physics Department, Faculty of Science, Galala University, Egypt

3 Ass.Professor of Orthodontics, Orthodontic Department, Faculty of Dentistry, Minia University

Abstract

Background:
There is a lack of data in regard to sterilization effects on titanium alloy mini screws and subsequent changes in their mechanical properties and clinical function. However none of these researches investigated the effects of repeated sterilization on antibacterial characteristics of coated orthodontic mini screws with different agents. Accordingly, the aim of this study was to evaluate the effects of sterilization procedures on the antibacterial characteristics of zinc oxide nanoparticles coated mini screws.
Methods:
A steam autoclave was used to sterilize a group of fifteen zinc oxide nano coated Self-drilling Ti6 Al4 V alloy mini screws. SEM (Scanning electron microscope) and EDAX (Energy-dispersive X-ray analysis) analysis were used to analyze the ZnO nanoparticles morphology and chemical composition of the particles after sterilization . Antibacterial activity of the sterilized and non-sterilized coated mini screws was compared against Streptococcus mutans (Gram-positive), Staphylococcus aureus (S.aureus) (Gram-positive) and Escherichia coli (E. coli) (Gram-negative).
Results:
According to measurements of the zone of inhibition of the test samples for quantitative assessment, the area of the zone of inhibition for S.aureus around (25 ± 4.88 mm ) was the largest with non-sterilized ZnONP coated mini screws, followed by that of S. mutans ; no zone of inhibition was observed for E.coli. The sterilized mini screws showed no antibacterial effects on both S. mutans and E-coli test bacteria, only S.aureus showed smallest zone of inhibition.
Conclusion:
Sterilization of coated ZnO mini screws has weak effect on antibacterial properties, making it possible to sterilize it before screw insertion.

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Egyptian Dental Journal
Volume 71, Issue 1 - Serial Number 1
January (Orthodontics, Pediatric & Preventive Dentistry)
January 2025
Pages 1-11

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